Qhov Tseem Ceeb - Proteomics vs Transcriptomics
Lub thev naus laus zis omic yog qhov tam sim no, qhov sib txawv biomolecules ntawm cov kab mob raug saib raws li tag nrho cov sau txog nws cov khoom thiab kev ua haujlwm. Lub omic technology muaj ntau yam kev siv. Qhov sib txawv omics ntawm cov qauv lom muaj xws li genomics, proteomics, transcriptomics thiab metabolomics. Proteomics suav nrog kev kawm tiav ntawm tag nrho cov proteins hauv cov kab mob muaj sia. Nws txhais tau tias yog cov txheej txheem ntawm tag nrho cov proteins uas qhia hauv lub cev, nws cov qauv thiab kev ua haujlwm. Ua tiav cov proteins, yog li, tsim cov proteome. Transcriptomics yog kev kawm tiav ntawm tag nrho cov tub txib RNA (mRNA) molecules tam sim no nyob rau hauv lub cev muaj sia. Yog li, transcriptomics cuam tshuam nrog cov noob uas nquag qhia hauv lub cev muaj sia. Tag nrho cov txheej txheem ntawm mRNA nyob rau hauv ib lub cev muaj sia yog hu ua transcriptome. Qhov sib txawv tseem ceeb ntawm Proteomics thiab Transcriptomics yog nyob ntawm hom biomolecule. Nyob rau hauv proteomics, tag nrho cov txheej txheem ntawm cov proteins nyob rau hauv ib tug muaj sia nyob yog kawm whereas, nyob rau hauv transcriptomics, tag nrho mRNA ntawm ib tug muaj sia nyob yog kawm.
YProteomics yog dab tsi?
Lo lus proteomics tau tsim nyob rau xyoo 1995 thiab tau pib txhais tias yog tag nrho cov protein ntxiv hauv cov cell, cov ntaub so ntswg lossis kab mob. Nrog rau kev nce qib hauv cov kev tshawb fawb proteomic, nws tau hloov kho kom raug suav tias yog lub kaus mom uas muaj ntau yam kev kawm suav nrog. Tam sim no, nyob rau hauv cov ncauj lus proteomics, cov qauv, kev taw qhia, kev ua haujlwm, kev sib cuam tshuam, nws cov kev hloov kho, nws cov ntawv thov thiab qhov tseem ceeb ntawm cov protein tau kawm. Yog li ntawd, ntau kev tshawb fawb tau ua nyob rau hauv lub teb ntawm proteomics tam sim no.
Thawj qhov kev tshawb fawb proteomic tau ua tiav los txheeb xyuas cov ntsiab lus protein hauv Escherichia coli. Daim ntawv qhia ntawm tag nrho cov ntsiab lus protein tau ua tiav siv ob qhov (2D) gels. Raws li qhov ua tiav ntawm qhov no, cov kws tshawb fawb tau txav mus rau yam ntxwv ntawm tag nrho cov ntsiab lus protein hauv cov tsiaj xws li guinea npua thiab nas. Tam sim no, tib neeg daim ntawv qhia protein yog ua tiav siv 2D gel electrophoresis.
Daim ntawv thov ntawm Proteomics
Muaj ntau qhov zoo ntawm kev kawm proteomics, vim tias cov proteins yog cov tswj hwm cov molecules ntawm feem ntau ntawm cov haujlwm vim yog cov cuab yeej catalyst ntawm cov protein. Yog li, kev kawm ntawm tag nrho cov proteins tuaj yeem muab cov ntaub ntawv hais txog kev noj qab haus huv ntawm lub cev. Qee daim ntawv thov yog;
- Genome annotation: Los ntawm kev kawm txog cov ntsiab lus protein ntawm cov kab mob, cov genomes lub luag haujlwm rau cov khoom siv protein ntau tuaj yeem txiav txim siab. Hauv qhov xwm txheej no tau tshwm sim los ntawm tag nrho cov genomics, Transcriptomics thiab proteomics yog qhov tseem ceeb.
- Kev txheeb xyuas kab mob / Kev kuaj mob: Proteomics yog siv los txheeb xyuas tus kab mob, los ntawm kev sib piv cov kev noj qab haus huv thiab cov kab mob
- Yuav kom ua tiav cov lus qhia protein tau kawm thaum lub sijhawm sim.
- Kev hloov pauv cov protein thiab kev sib cuam tshuam kev kawm: Txhawm rau siv cov proteins hauv vitro mob lossis thiab hauv vivo, txiav txim siab cov xwm txheej khaws cia ntawm cov proteins uas tau muab rho tawm thiab kawm txog tus cwj pwm ntawm cov protein hauv vitro, hauv vivo thiab hauv - cov txheej txheem silico.
Daim duab 01: Proteomics
Muaj cov txheej txheem sib txawv koom nrog hauv proteomics
- Kev rho tawm tag nrho cov protein thiab cais cov proteins uas siv 2D gel electrophoresis. Proteins kuj tseem tuaj yeem sib cais siv High-Performance Liquid Chromatography (HPLC).
- Kev sib cais ntawm cov proteins uas tau muab rho tawm siv txoj hauv kev xws li Edmund txoj kev sib txuas lossis pawg spectrometry.
- Thaum qhov kev txheeb xyuas tau txheeb xyuas, cov qauv tsim thiab kev ua haujlwm ntawm cov ntsiab lus protein raug tshuaj xyuas siv computer-based software thiab bioinformatics cov cuab yeej.
Transcriptomics yog dab tsi?
Cov lus sau tseg tau muab sau ua ib ntus tsis ntev los no. Transcriptomics yog kev tshawb fawb txog tag nrho mRNA cov ntsiab lus ntawm cov kab mob. Tag nrho mRNA yog cov DNA qhia hauv cov kab mob uas muaj sia nyob lossis ib lub cell. Kev sau tag nrho ntawm mRNA raug xa mus ua cov ntawv sau tseg.
Cov kauj ruam ntawm kev txheeb xyuas cov ntawv sau tseg suav nrog,
- Kev rho tawm ntawm RNA, sib cais ntawm mRNA siv kem gel chromatography nrog poly DT hlaws.
- Kev ua ntu zus ntawm mRNA ua tiav.
Microarray thev naus laus zis yog ib txoj hauv kev los txheeb xyuas cov ntaub ntawv ntawm cov kab mob. Cov txheej txheem microarray cuam tshuam nrog kev sojntsuam phaj nrog cov hlua txuas ntxiv ntawm cov ntawv sau tseg. Thaum hybridization, mRNA tam sim no nyob rau hauv lub cev los yog hlwb tuaj yeem ua tus yam ntxwv.
Daim duab 02: Cov Txheej Txheem Tshaj Tawm
Transcriptomics tam sim no tau siv dav hauv kev kho mob. Kev kuaj kab mob thiab kab mob profileing yog cov teb tseem ceeb uas siv Transcriptomics. Los ntawm kev txheeb xyuas cov ntaub ntawv ntawm cov kab mob, txawv teb chaws mRNA tuaj yeem txheeb xyuas, thiab yog tias muaj kab mob, nws tuaj yeem txheeb xyuas tau. Qhov tsis-coding RNA tuaj yeem sib cais siv cov thev naus laus zis thev naus laus zis. Thiab tseem qhia txog cov noob nyob rau hauv ib puag ncig kev ntxhov siab sib txawv tuaj yeem saib xyuas.
Qhov Zoo sib xws ntawm Proteomics thiab Transcriptomics yog dab tsi?
- Ob leeg tsim ib feem ntawm lub tswvyim ntawm omic technology.
- Ob leeg yog siv rau hauv kev kuaj kab mob thiab kab mob tus yam ntxwv ntawm lub cev.
- Ob qhov chaw kawm koom nrog kev rho tawm ntawm biomolecule, kev sib cais ntawm biomolecule thiab sequencing cov kauj ruam.
Qhov txawv ntawm Proteomics thiab Transcriptomics yog dab tsi?
Protemics vs Transcriptomics |
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| Proteomics suav nrog kev kawm tiav ntawm tag nrho cov proteins hauv lub cev muaj sia. | Transcriptomics yog kev kawm tiav ntawm tag nrho cov tub txib RNA (mRNA) molecules tam sim no nyob rau hauv lub cev muaj sia. |
| Studied Bio Molecule Type | |
| Proteins tau kawm hauv proteomics. | mRNA tau kawm hauv kev sau ntawv. |
| Cov Ntsiab Lus Kawm | |
| qauv, kev ua haujlwm, kev sib cuam tshuam, kev hloov kho thiab kev siv cov proteins tau kawm hauv proteomics. | Cov qauv sib txuas, kev cuam tshuam nrog ib puag ncig thiab kev siv ntawm mRNA tau kawm hauv kev sau ntawv. |
Summary – Proteomics vs Transcriptomics
Omics ua lub luag haujlwm tseem ceeb hauv kev tshawb fawb txog lub neej. Proteomics yog hais txog kev tshawb fawb ntawm cov proteome uas tsim cov kev sau tag nrho ntawm cov proteins hauv ib lub xovtooj ntawm tes lossis cov kabmob. Transcriptomics yog hais txog kev tshawb fawb ntawm cov ntaub ntawv transcriptome uas yog tag nrho cov txheej txheem ntawm DNA uas yog nyob rau hauv daim ntawv ntawm mRNA. Ob qhov kev kawm, proteomics thiab transcriptomics, tau muab los tom qab kev qhia txog genomics thiab tam sim no siv dav hauv kev kuaj mob thiab hauv kev ua yeeb yam thiab tshuaj ntsuam kab mob. Qhov no yog qhov txawv ntawm proteomics thiab transcriptomics.
Download PDF ntawm Proteomics vs Transcriptomics
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov rub tawm PDF version ntawm no: Qhov txawv ntawm Proteomics thiab Transcriptomics
