Qhov sib txawv tseem ceeb ntawm proteomics thiab metabolomics yog qhov proteomics yog kev tshawb fawb ntawm tag nrho cov proteins ntawm cov kab mob thaum metabolomics yog kev kawm ntawm tag nrho cov metabolites ntawm lub cev.
Genomics yog kev kawm txog cov caj ces tsim ntawm ib yam kabmob. Proteomics thiab metabolomics yog ob txoj kev tshawb fawb omic ntsig txog genomics. Proteome yog hais txog tag nrho cov proteins nyob rau hauv lub cell los yog ib tug kab mob. Metabolome, ntawm qhov tod tes, hais txog tag nrho cov metabolites ntawm lub cev. Yog li, kev tshawb fawb ntawm proteome yog cov proteomics thaum txoj kev tshawb fawb ntawm metabolome yog cov metabolomics. Ob qho tib si proteomics thiab metabolomics yog qhov tseem ceeb hauv kev kuaj kab mob thiab hauv kev ua yeeb yam thiab kev tshuaj xyuas cov kab mob. Tsis tas li ntawd, ob qho tib si txuas nrog ntau yam ntawm cell biology, tshwj xeeb tshaj yog nyob rau hauv cell signaling, protein degradation thiab tiam thiab post-translational modification.
YProteomics yog dab tsi?
Proteome yog tag nrho cov protein ntxiv hauv cov cell, cov ntaub so ntswg lossis kab mob. Yog li, proteomics yog txoj kev tshawb fawb ntawm proteome ntawm lub cev. Hauv proteomics, cov yam ntxwv sib txawv ntawm cov protein raug tshuaj xyuas. Yog li ntawd, proteomics tsuas yog tsom rau cov qauv, kev taw qhia, kev ua haujlwm, kev sib tshuam ntawm cov protein, kev hloov kho, kev siv thiab qhov tseem ceeb ntawm cov protein. Tias yog vim li cas ntau txoj haujlwm tshawb fawb tau ua hauv thaj tsam ntawm proteomics tam sim no.
Thawj qhov kev tshawb fawb proteomic tau ua tiav los txheeb xyuas cov ntsiab lus protein hauv Escherichia coli. Daim ntawv qhia ntawm tag nrho cov ntsiab lus protein tau ua tiav siv ob qhov (2D) gels. Tom qab ua tiav ntawm txoj haujlwm no, cov kws tshawb fawb tau txav mus rau yam ntxwv ntawm tag nrho cov ntsiab lus protein hauv cov tsiaj xws li guinea npua thiab nas. Tam sim no, tib neeg daim ntawv qhia protein yog ua tiav siv 2D gel electrophoresis.
Muaj ntau qhov zoo ntawm kev kawm proteomics vim cov proteins yog cov tswj hwm ntawm cov haujlwm feem ntau vim yog cov cuab yeej catalyst ntawm cov protein. Yog li, kev kawm ntawm tag nrho cov proteins tuaj yeem muab cov ntaub ntawv hais txog kev noj qab haus huv ntawm lub cev. Ntxiv mus, proteomics muaj ntau daim ntawv thov hauv genome annotation, kev txheeb xyuas kab mob thiab kev kuaj mob, ua cov kev tshawb fawb qhia protein thaum lub sij hawm sim thiab hloov cov protein thiab kev tshawb fawb sib cuam tshuam, thiab lwm yam.
Daim duab 01: Proteomics
Muaj ntau cov txheej txheem koom nrog hauv proteomics, suav nrog kev rho tawm tag nrho cov protein, sib cais ntawm lawv siv 2D gel electrophoresis, sequencing ntawm cov extracted proteins siv txoj kev xws li Edmund txoj kev sequencing los yog loj spectrometry thiab tsom xam ntawm cov qauv thiab kev ua haujlwm. Cov khoom ntawm cov protein siv computer-based software thiab bioinformatics cov cuab yeej.
YMetabolomics yog dab tsi?
Metabolomics yog kev tshawb fawb ntawm tag nrho cov metabolites hauv lub cell, cov ntaub so ntswg los yog cov kab mob. Nws suav nrog kev txheeb xyuas thiab ntsuas cov cellular metabolites siv cov cuab yeej ntsuas qib siab sib txawv. Metabolomics yog ib qho kev kawm tseem ceeb vim nws nthuav tawm cov ntaub ntawv hais txog cov metabolism hauv lub cev.
Daim duab 02: Metabolomics
Txhawm rau kawm substrates thiab cov khoom ntawm cov tshuaj tiv thaiv metabolic, metabolomics siv huab hwm coj spectrometry ua ib qho kev soj ntsuam platform. Mass spectrometry ua rau cov metabolites thiab lawv cov concentrations, xav txog qhov tseeb biochemical lub xeev ntawm cov hlwb los yog cov ntaub so ntswg. Yog li, metabolomics tuaj yeem suav hais tias yog qhov zoo tshaj plaws sawv cev ntawm molecular phenotype ntawm lub cev. Ntxiv rau cov no, metabolomics tuaj yeem suav hais tias yog qhov txuas ntxiv ntawm proteomics vim tias feem ntau cov metabolites raug tsim los ntawm cov dej num ntawm cov enzymes uas yog cov proteins.
Dab tsi yog qhov zoo sib xws ntawm Proteomics thiab Metabolomics?
- Proteomics thiab metabolomics yog ob txoj kev tshawb fawb omic ntsig txog genomics.
- Metabolomics tuaj yeem suav tias yog qhov txuas ntxiv ntawm proteomics.
- Proteome thiab metabolome yog sib cuam tshuam nrog ntau txoj kev tshawb fawb.
Qhov txawv ntawm Proteomics thiab Metabolomics yog dab tsi?
Proteomics yog qhov kev kawm loj ntawm cov proteins nrog rau lawv cov qauv thiab kev ua haujlwm, hauv ib lub xovtooj ntawm tes lossis cov kabmob. Meanwhile, metabolomics yog txoj kev tshawb fawb ntawm metabolite profiles nyob rau hauv ib lub cell, cov ntaub so ntswg los yog kab mob nyob rau hauv ib tug muab tej yam kev mob. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm proteomics thiab metabolomics. Tsis tas li ntawd, proteomics feem ntau txhawj xeeb txog cov txheej txheem tag nrho ntawm cov proteins thaum metabolomics feem ntau cuam tshuam txog cov txheej txheem metabolites, suav nrog ntau hom me me xws li peptides, carbohydrates, lipids, nucleosides, thiab catabolic cov khoom ntawm exogenous compounds, thiab lwm yam. Yog li, qhov no yog lwm qhov sib txawv ntawm proteomics thiab metabolomics.
Cov hauv qab no qhia txog qhov sib txawv ntawm proteomics thiab metabolomics.
Summary – Proteomics vs Metabolomics
Cov kab mob muaj sia tuaj yeem kawm siv lawv cov genome, proteins thiab metabolites. Genomics, proteomics thiab metabolomics yog peb qhov chaw uas kawm genetic make-up, proteins thiab metabolites ntawm ib lub xovtooj ntawm tes los yog ib yam kabmob, feem. Proteomics yog ib qho tseem ceeb vim nws yog ib qho kev xav ntawm ob qho tib si noob thiab ib puag ncig. Metabolomics yog qhov tseem ceeb heev vim nws ncaj qha cuam tshuam txog cov xwm txheej tam sim no ntawm cov tshuaj tiv thaiv biochemical ntawm cov hlwb lossis cov txheej txheem metabolic ntawm lub cev. Yog li, qhov no qhia txog qhov sib txawv ntawm proteomics thiab metabolomics.
