Qhov Tseem Ceeb - Metabolomics vs Metabonomics
Metabolites yog cov molecules me me koom nrog hauv cov tshuaj tiv thaiv metabolic hauv cov hlwb. Cov metabolites muaj xws li metabolic intermediates, cov tshuaj hormones, theem nrab metabolites, signaling molecules, thiab lwm yam. Ib qho kev ua tiav ntawm cov metabolites ntawm cov qauv lom neeg lossis ib yam kabmob hu ua metabolome. Metabolome yog ib qho kev sib sau ua ke uas hloov txhua thib ob hauv lub cev. Metabolomics thiab metabonomics yog ob nqe lus hais txog kev tshawb fawb ntawm metabolome ntawm cov kab mob. Qhov sib txawv tseem ceeb ntawm cov tshuaj metabolomics thiab metabonomics yog tias cov tshuaj metabolomics muaj kev txhawj xeeb ntau dua txog cov txheej txheem endogenous metabolism thiab metabolic profiling ntawm qib cellular lossis hauv nruab nrog cev thaum metabonomics muaj kev txhawj xeeb ntxiv nrog kev nthuav dav metabolic profiling nrog cov ntaub ntawv ntawm perturbations ntawm metabolism vim ib puag ncig yam, kab mob, plab. microorganisms thiab sib piv metabolic profiling, thiab lwm yam. Metabolomics feem ntau yog tsav los ntawm huab hwm coj spectrometry, thiab metabonomics siv NMR spectroscopy rau metabolite tsom xam.
YMetabolomics yog dab tsi?
Metabolomics yog kev kawm txog cov txheej txheem metabolic uas tshwm sim hauv hlwb, biofluids, cov ntaub so ntswg lossis kab mob. Nws suav nrog kev txheeb xyuas thiab txheeb xyuas cov cellular metabolites siv cov cuab yeej tshuaj ntsuam xyuas zoo thiab txheeb xyuas. Metabolomics yog suav tias yog ib qho kev tshawb fawb tseem ceeb vim nws qhia cov ntaub ntawv hais txog cov metabolism hauv lub cev.
Txhawm rau kawm substrates thiab cov khoom ntawm cov tshuaj tiv thaiv metabolic, metabolomics siv huab hwm coj spectrometry ua ib qho kev soj ntsuam platform. Mass spectrometry qhia txog hom metabolites thiab lawv cov concentrations, qhia txog qhov tseeb biochemical lub xeev ntawm cov hlwb los yog cov ntaub so ntswg. Yog li, metabolomics tuaj yeem suav hais tias yog tus sawv cev zoo tshaj plaws ntawm molecular phenotype ntawm lub cev. Metabolomics yog ib qho omic technology ntawm me me molecules ntawm cov kab mob thiab piv rau lwm yam kev tshawb fawb omic; metabolomics tseem ceeb heev vim nws ncaj qha cuam tshuam txog cov xwm txheej tam sim no ntawm biochemical cov tshuaj tiv thaiv ntawm cov hlwb.
Metabolomics tseem tuaj yeem suav tias yog qhov txuas ntxiv ntawm proteomics vim tias lawv raug tsim los ntawm kev ua haujlwm ntawm cov enzymes uas yog cov proteins raws li qhia hauv daim duab hauv qab no.
Daim duab 01: Metabolomics scheme
Metabonomics yog dab tsi?
Metabonomics txhais tau tias yog qhov ntsuas qhov ntau ntawm cov lus teb ntawm cov lus teb ntawm cov metabolic nyob rau lub sijhawm tshwj xeeb hauv kev cuam tshuam txog kev ntxhov siab lossis kev hloov caj ces. Qhov no feem ntau yog siv rau kev tshawb fawb txog tib neeg kev noj haus thiab cov lus teb rau cov tshuaj lossis kab mob. Txoj hauv kev no yog pioneered los ntawm Jeremy Nicholson ntawm Imperial College, London thiab nws tau siv nyob rau hauv ib tug xov tooj ntawm cov teb xws li toxicology, kab mob, kev noj haus, thiab lwm yam. Keeb kwm, metabonomics yog ib tug ntawm cov thawj txoj kev siv lub Scope ntawm systems biology rau kev kawm. ntawm cov metabolism.
Metabonomics muaj kev txhawj xeeb ntau dua txog kev sib piv biochemical profile es tsis yog txheeb xyuas tus kheej cov tshuaj metabolic hauv cov metabolomics. Yog li, metabonomics tuaj yeem txhais tau tias yog cov txheej txheem metabolomics. Nws tsuas yog hais txog kev sib piv ntawm cov kab mob metabolic ntawm cov neeg sib txawv nrog rau cov kab mob, kev ntxhov siab ib puag ncig, kev hloov pauv caj ces, khoom noj khoom haus, tshuaj, thiab lwm yam vim tias cov metabolites yog cov cim zoo uas qhia txog cov kab mob, cov teebmeem ntawm cov tshuaj, ib puag ncig kev nyuaj siab, raug rau co toxins., etc.
Kev tshawb fawb Metabonomic qhia ob qho tib si hauv cov cellular thiab extracellular metabolites. Nws siv cov txheej txheem siab dhau los txhawm rau txheeb xyuas ntau tus lej ntawm cov metabolites ib txhij thiab ntau zaus, ua rau kev tshawb nrhiav lub cev ntawm lub cev ntawm cov hlwb. Nws kuj qhia txog kev sib raug zoo ntawm metabolites thiab pathological txheej xwm ntawm lub cev. Txhawm rau txhawb kev tshawb fawb metabonomic, genomics thiab proteomics tuaj yeem siv tau vim tias lawv muaj kev cuam tshuam raws li qhia hauv daim duab saum toj no.
Qhov txawv ntawm Metabolomics thiab Metabonomics yog dab tsi?
YMetabolomics vs Metabonomics |
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| Metabolomics yog txoj kev tshawb fawb ntawm tag nrho cov txheej txheem metabolites ntawm cov kab mob | Metabonomics yog qhov kev tshawb fawb ntau ntawm cov lus teb ntau ntawm cov metabolic ntawm cov kab mob nyob hauv lub cev mus rau pathophysiological stimuli lossis kev hloov kho caj ces. |
| Kev txhawj xeeb tseem ceeb | |
| Metabolomics muaj kev txhawj xeeb ntau dua nrog cov metabolic profiling thiab txheeb xyuas tus neeg metabolites uas muaj nyob hauv cov hlwb. | Metabonomics muaj kev txhawj xeeb ntau dua nrog kev sib piv cov metabolites lossis metabolic profiles ntawm cov pejxeem hauv kev cuam tshuam nrog kev hloov pauv caj ces, kab mob, kev ntxhov siab ib puag ncig, kev tawm tsam pathological, tshuaj, thiab lwm yam. |
| Information | |
| Metabolomics yog tsom rau cov metabolism hauv endogenous. | Metabonomics tsis txwv rau cov metabolism hauv endogenous. Nws txuas ntxiv mus nrhiav cov ntaub ntawv hais txog kev cuam tshuam ntawm cov metabolism los ntawm sab hauv thiab sab nraud xws li cov qauv kev noj haus, co toxins, txheej txheem kab mob, thiab lwm yam. |
| Cov cuab yeej ntsuas ntsuas | |
| Nws siv huab hwm coj spectrometry ua tus thawj coj tsom xam platform | Metabonomics siv NMR spectroscopy ua lub ntsiab analytical platform. |
Summary – Metabolomics vs Metabonomics
Metabolome sawv cev rau tag nrho cov me me molecules hu ua metabolites tam sim no nyob rau hauv ib lub cell los yog nyob rau hauv lub cev. Metabolomics yog kev tshawb fawb ntawm tag nrho cov metabolome los tsim cov metabolic profile. Metabolomics tso cai rau cov kws tshawb fawb los ntsuas qhov tseeb ntawm lub cev ntawm lub cev lossis lub cev. Metabonomics yog ib feem ntawm cov metabolomics uas txuas ntxiv mus rho tawm cov ntaub ntawv hais txog cov lus teb multiparametric metabolic ntawm cov kab mob nyob rau hauv pathophysiological stimuli thiab kev hloov kho caj ces. Metabonomics tsis yog kev txhawj xeeb ntawm tus kheej metabolic profiling zoo li metabolomics; Nws sib piv cov metabolic profiles nrog rau lwm yam xws li ib puag ncig kev nyuaj siab, kab mob, co toxins, thiab lwm yam. Ob leeg metabolomics thiab metabonomics koom nrog cov kev tshawb fawb ntawm metabolites ntawm cov kab mob los ntsuas qhov tseeb physiological txheej txheem ntawm cov hlwb. Li no, qee zaum ob qhov no suav hais tias yog cov lus sib piv, tsis xav txog qhov sib txawv ntawm metabolomics thiab metabonomics.
