Qhov sib txawv tseem ceeb ntawm Luminol thiab Bluestar yog tias Luminol yog qhov sib piv qis dua, qhov Bluestar yog qhov sib piv ntau ruaj khov.
Luminol thiab Bluestar yog cov khoom tseem ceeb hauv cov txheej txheem kuaj mob. Bluestar yog luminol-raws li reagent. Nws muaj txiaj ntsig zoo hauv kev kuaj ntshav rau kev tshawb nrhiav. Qhov tseem ceeb, Bluestar yog kim dua Luminol vim nws muaj peev xwm muab lub zog fluorescence rau lub sijhawm ntev.
YLuminol yog dab tsi?
Luminol yog cov tshuaj lom neeg uas tuaj yeem pom cov chemiluminescence nrog cov xim xiav ci. Nws muab cov fluorescence no thaum sib tov ib yam khoom uas tsim nyog oxidizing tus neeg sawv cev. Cov tshuaj no tshwm sim raws li ib tug dawb-rau-daj-daj-daj crystalline khoom. Luminol yog dej-insoluble, tab sis nws yog soluble nyob rau hauv ntau polar organic solvents.
Daim duab 01: Cov Qauv Tshuaj ntawm Luminol
Cov khoom no yog ib qho tseem ceeb hauv cov txheej txheem kev tshawb nrhiav kev tshawb nrhiav kev tshawb nrhiav cov ntshav me me uas tau sau rau ntawm qhov chaw ua txhaum cai. Luminol tuaj yeem cuam tshuam nrog hlau hauv hemoglobin hauv cov ntshav. Yog li ntawd, peb tuaj yeem siv cov tshuaj no hauv ntau cov xov tooj ntawm tes kom pom kev zoo li kev kuaj tus tooj liab, thiab qee cov protein blotting.
Yog tias peb txau Luminol sib npaug thoob ib cheeb tsam, qhov me me ntawm cov oxidant ua kom muaj zog tuaj yeem ua rau Luminol tawm xim xiav xim fluorescence ci uas tshwm hauv chav tsaus. Qhov ci ci no feem ntau kav ntev li 30 vib nas this. Txawm li cas los xij, peb tuaj yeem yooj yim sau nws los ntawm kev yees duab. Yog hais tias peb tsuag ntau Luminol nyob rau hauv lub cheeb tsam, nws muab ib tug kaj xiav glow. Txawm li cas los xij, qhov kev siv ntawm qhov ci ntsa iab no tsis qhia txog qhov muaj ntshav hauv thaj tsam ntawd.
Daim duab 02: Xiav Glow ntawm Luminol
Peb tuaj yeem tsim cov Luminol los ntawm cov txheej txheem ob kauj ruam, uas pib nrog 3-nitrophthalic acid. Raws li thawj kauj ruam, peb yuav tsum tau cua sov hydrazine nrog 3-naphthalic acid nyob rau hauv lub xub ntiag ntawm ib tug high nqi hnyav hnyav, xws li triethylene glycol thiab glycerol. Ntawm no, ib qho kev hloov pauv acyl condensation tshwm sim. Yog li ntawd, cov dej molecule poob los ntawm cov kauj ruam cov tshuaj tiv thaiv thiab tsim 3-nitrophthalhydrazide. Lumino cov ntaub ntawv los ntawm kev txo cov nitro pawg mus rau ib pawg amino nyob rau hauv muaj sodium dithionite. Qhov no tshwm sim ntawm qhov nruab nrab hu ua hydroxylamine.
Bluestar yog dab tsi?
Bluestar yog tus tsim cov ntshav zoo tshaj plaws thiab kuaj pom siv ntawm qhov chaw ua txhaum cai lossis hauv chav kuaj. Nws yog ib hom luminol-raws li reagent. Cov reagent no muaj qhov rhiab heev, uas tso cai rau cov txheej txheem tshawb nrhiav kom pom cov kab mob ntawm cov ntshav nrog qhov muag liab qab. Ntxiv mus, Bluestar ruaj khov dua Luminol. Tab sis nws yog kim dua Luminol vim nws muaj peev xwm muab lub zog fluorescence rau lub sijhawm ntev. Qhov no yog ib qho tseem ceeb ntawm kev txhim kho cov ntshav reagent thiab tuaj yeem qhia cov ntshav stains uas tau ntxuav tawm, so tawm, lossis pom tsis pom qhov muag liab qab. Cov reagent no tau tsim tshwj xeeb nrog rau lub hom phiaj ntawm kev siv nws ntawm kev tshawb nrhiav kev ua txhaum cai.
Qhov txawv ntawm Luminol thiab Bluestar yog dab tsi?
Bluestar yog luminol-raws li reagent. Nws muaj txiaj ntsig zoo hauv kev kuaj ntshav rau kev ua txhaum cai. Qhov sib txawv tseem ceeb ntawm Luminol thiab Bluestar yog tias Luminol yog qhov sib piv tsis tshua ruaj khov, thaum Bluestar yog qhov sib piv ruaj khov dua. Ntxiv mus, Bluestar yog kim dua Luminol vim nws muaj peev xwm muab lub zog fluorescence rau lub sij hawm ntev.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm Luminol thiab Bluestar hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – Luminol vs Bluestar
Luminol thiab Bluestar yog cov khoom tseem ceeb hauv cov txheej txheem kuaj mob. Qhov sib txawv tseem ceeb ntawm Luminol thiab Bluestar yog tias Luminol yog qhov sib piv tsis tshua ruaj khov, thaum Bluestar yog qhov sib piv ruaj khov dua.
