Qhov sib txawv tseem ceeb ntawm cov phiaj xwm thiab tsis muaj kev tiv thaiv yog tias cov phiaj xwm proteomics tsom rau kev ntsuas qhov nplua nuj ntawm cov protein tshwj xeeb thaum cov neeg tsis tau pom zoo proteomics tsis tsom rau cov protein tshwj xeeb.
Proteomics yog qhov kev kawm loj ntawm cov protein, lawv cov qauv, thiab kev ua haujlwm. Nws kawm tag nrho cov protein ntxiv nyob rau hauv ib lub cell, cov ntaub so ntswg los yog cov kab mob nyob rau hauv tej yam kev mob. Muaj ob hom proteomics raws li hom phiaj proteomics thiab untargeted proteomics.
Lub Hom Phiaj Proteomics yog dab tsi?
Raws li nws lub npe qhia, lub hom phiaj proteomics tsom rau cov protein tshwj xeeb lossis peptide hauv kev sib xyaw ua ke ntawm cov proteins rau kev tshuaj xyuas. Tom qab ntawd nws txheeb xyuas qhov muaj thiab qhov ntau ntawm cov protein uas tau xaiv ua ntej. Txoj kev no tsuas yog ua tau rau ib qho qauv lossis hla ntau qhov qauv. Txoj kev no yog tsim txawm rau ib pab pawg neeg ntawm cov proteins. Nws ua rau cov ntaub ntawv meej dua, ntau thiab muaj txiaj ntsig. Ntxiv mus, huab hwm coj spectrometry ua ke nrog cov phiaj xwm proteomics tsim cov kev ntsuas tseeb dua. Txoj kev no siv triple quadrupole mass spectrometer (QQQ) rau nws qhov kev tshuaj ntsuam.
Daim duab 01: Proteomics
Targeted proteomics yog ib txoj hauv kev tsim nyog yog tias koj xav txheeb xyuas yog tias lub hom phiaj knockdown puas tiag tiag lossis thaum koj xav kom suav cov protein / peptide hauv cov ntshav ntshav, kua hauv hlwb lossis hauv cell lysate thiab lwm yam. Koj tuaj yeem ua tau. siv nws yog tias koj xav tshawb xyuas seb cov protein tshwj xeeb puas txawv ntawm cov qauv los yog thaum lub sij hawm kev sim hauv chav kawm.
Dab tsi yog Kev Tiv Thaiv Tsis Tiv Thaiv?
Untargeted proteomics, raws li lub npe hais, tsis tsom ib qho protein lossis peptide rau nws qhov kev tshuaj xyuas. Discovery proteomics yog lwm lub npe rau Untargeted proteomics. Qhov tseeb, nws yog qhov kev soj ntsuam ntau dua 'ntiaj teb'. Feem ntau, kev ua haujlwm tsis muaj kev tiv thaiv yog los teb cov lus nug "Yuav ua li cas cov protein lossis cov protein nyob hauv cov qauv no lossis ntau cov qauv?". Txoj kev no tsis yog rhiab raws li cov hom phiaj proteomics. Tab sis nws tuaj yeem txheeb xyuas thiab ntsuas ntau cov proteins suav nrog cov protein tshiab. Untargeted proteomics tuaj yeem tsim ob qho kev ntsuas ntau thiab zoo.
Dab tsi yog qhov zoo sib xws ntawm Lub Hom Phiaj thiab Tsis Tau Pom Zoo Proteomics?
- Ob lub hom phiaj thiab tsis muaj kev tiv thaiv proteomics ntsuas cov protein hauv cov qauv.
- Lawv yog qhov tseem ceeb hauv kev tsom xam proteome.
- Cov txheej txheem no siv huab hwm coj spectrometry.
Qhov txawv ntawm Lub Hom Phiaj thiab Tsis Tau Pom Zoo Proteomics yog dab tsi?
Ua ntej, lub hom phiaj proteomics yog qhov kev txiav txim siab ntawm qhov muaj thiab muaj pes tsawg leeg ntawm ib qho protein lossis peptide hauv kev sib xyaw ntawm cov protein. Hauv qhov sib piv, cov khoom siv tsis sib haum xeeb yog qhov kev tshawb fawb ntau thiab zoo ntawm cov protein uas muaj nyob hauv cov qauv lossis cov qauv yam tsis tau tsom rau ib qho protein. Targeted proteomics yog ua nrog preselected los yog targeted proteins thaum untargeted proteomics yog ua los xyuas seb hom proteins nyob rau hauv cov qauv no. Ntxiv mus, lub hom phiaj proteomics yog rhiab ntau dua li cov proteomics uas tsis tau pom dua.
Summary – Targeted vs Untargeted Proteomics
Yuav kom suav, cov hom phiaj thiab tsis muaj kev tiv thaiv proteomics yog ob hom kev tshawb fawb protein. Raws li lawv cov npe qhia, cov phiaj xwm proteomics tsom rau cov protein tshwj xeeb thaum cov tshuaj tiv thaiv tsis muaj zog tsis tsom rau cov protein tshwj xeeb. Qhov no yog qhov sib txawv ntawm cov hom phiaj thiab cov neeg tsis tau pom zoo.
