Qhov Tseem Ceeb Qhov txawv - Epitope vs Paratope
Immunological cov tshuaj tiv thaiv muaj nyob rau hauv teb rau kev cuam tshuam los ntawm txawv teb chaws lub cev, tshwj xeeb tshaj yog cov kab mob sib kis. Kev tiv thaiv kab mob tuaj yeem yog ob hom sib txawv; nonspecific mechanisms thiab tshwj xeeb mechanisms. Cov txheej txheem tiv thaiv kab mob tshwj xeeb koom nrog cov tshuaj tiv thaiv ntawm cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv uas ua rau muaj kev puas tsuaj ntawm lub cev txawv teb chaws. Antibody-antigen cov tshuaj tiv thaiv yog kho los ntawm kev sib cuam tshuam tsis muaj zog xws li kev sib cuam tshuam ionic, kev sib cuam tshuam hydrophobic, thiab kev cuam tshuam ntawm Van der Waals. Qhov chaw tseem ceeb ntawm cov tshuaj tiv thaiv kab mob thiab cov antigen uas koom nrog cov tshuaj tiv thaiv yog Epitope thiab Paratope. Lub Epitope yog thaj chaw hauv cov tshuaj tiv thaiv ntawm lub cev txawv teb chaws uas khi rau cov tshuaj tiv thaiv kab mob thaum Paratope yog thaj chaw hauv cov tshuaj tiv thaiv uas khi rau cov tshuaj tiv thaiv. Qhov no yog qhov sib txawv tseem ceeb ntawm Epitope thiab Paratope. Epitope thiab Paratope koom nrog hauv kev tiv thaiv kab mob ntawm cov antigen thiab cov tshuaj tiv thaiv kab mob.
Epitope yog dab tsi?
Antigens tam sim no yog cov receptors hauv lub cev txawv teb chaws, thiab lawv yog cov cim uas tau txheeb xyuas los ntawm tus tswv tsev tiv thaiv kab mob. Ib qho epitope yog ib qho chaw tshwj xeeb hauv cov antigen, uas yog qhov chaw tshwj xeeb uas cov tshuaj tiv thaiv khi. Qhov kev khi no pib lub cev tiv thaiv kab mob thiab yog li ua rau kev puas tsuaj ntawm txawv teb chaws molecule. Feem ntau, ib qho epitope yog tsim los ntawm cov amino acid ib ntus ntawm li tsib mus rau rau cov amino acids ntev. Epitopes yog cov txheej txheem tertiary protein, thiab qhov no tau lees paub los ntawm x-ray crystallography txoj kev. Ib qho antigen tuaj yeem muaj ntau dua ib lossis ntau qhov epitopes tiv thaiv cov tshuaj tiv thaiv kab mob tuaj yeem khi. Qhov no ua rau cov tshuaj tiv thaiv sib txawv los khi rau ib qho antigen ib zaug. Kev khi ntawm cov tshuaj tiv thaiv kab mob thiab cov epitope tshwm sim ntawm Antigen Binding Site, uas yog hu ua Paratope thiab nyob rau ntawm qhov kawg ntawm thaj tsam sib txawv ntawm cov tshuaj tiv thaiv. Qhov Paratope no tsuas muaj peev xwm los khi nrog ib qho tshwj xeeb epitope.
Daim duab 01: Antigen-antibody khi ntawm Epitope.
Muaj ob yam tseem ceeb ntawm epitopes hauv cov ntsiab lus ntuj; nruam epitopes thiab discontinuous epitopes. Cov epitopes tsis tu ncua yog cov kab sib txuas ntawm cov amino acids whereas tsis tu ncua epitopes muaj nyob rau hauv tshwj xeeb conformations thiab folded nyob rau hauv sib txawv conformations.
Physiologically epitopes yog ntxiv cais raws li B reactive epitopes thiab T reactive epitopes. B reactive epitopes khi nrog B cell antibodies. T cell reactive epitopes khi nrog T cell thiab koom nrog kev tiv thaiv kab mob. Epitope mapping yog cov txheej txheem tshiab uas qhov chaw ntawm lub epitope raug txheeb xyuas txhawm rau txiav txim siab qhov xwm txheej ntawm cov tshuaj tiv thaiv kab mob. Los ntawm kev siv cov txheej txheem epitope mapping, hluavtaws epitopes tuaj yeem npaj tau raws li hauv vitro.
Paratope yog dab tsi?
Cov tshuaj tiv thaiv yog tsim los ntawm tus tswv tsev hauv kev teb rau kev cuam tshuam txawv teb chaws los ntawm kev lees paub cov chaw antigenic. Cov tshuaj tiv thaiv yog tsim los ntawm B hlwb, thiab lawv yog cov proteins tertiary hu ua immunoglobulins. Ib qho paratope kuj hu ua qhov chaw antigen-binding site, yog ib cheeb tsam tshwj xeeb lossis ib feem ntawm cov tshuaj tiv thaiv kab mob uas paub txog thiab khi rau thaj tsam epitope ntawm antigen Kev khi ntawm paratope mus rau epitope pib tiv thaiv kev tiv thaiv ntawm tus tswv tsev thiab cov invading. cev. Paratope yog ib cheeb tsam me me ntawm tsib txog kaum amino acids thiab yog 3D (3 seem) kev pom zoo.
Lub Paratope nyob hauv thaj av Fab lossis thaj tsam thaj tsam antigen-binding ntawm cov tshuaj tiv thaiv. Qhov no muaj cov khoom ntawm ob lub chains; cov saw hnyav thiab lub teeb saw ntawm cov qauv immunoglobulin. Txhua sab caj npab ntawm Y cov duab ntawm ib qho tshuaj tiv thaiv kab mob monomer yog tipped nrog ib tug paratope, uas yog ib txheej ntawm complementarity txiav txim cheeb tsam.
Dab tsi zoo sib xws ntawm Epitope thiab Paratope?
- Ob leeg yog tsim los ntawm cov amino acid sequences.
- Ob leeg koom nrog tshuaj tiv thaiv-antigen
- Qhov kev sib raug zoo ntawm ob lub qauv yog nyob ntawm lub zog ntawm kev nyiam thiab kev tawm tsam.
- Ob lub qauv tuaj yeem txheeb xyuas tau siv cov txheej txheem X-ray crystallographic.
- Ob lub qauv muaj peev xwm tsim kev sib txawv xws li H bonds, van der Waals rog, kev sib cuam tshuam ionic thiab kev sib cuam tshuam hydrophobic.
- Ob leeg tshwj xeeb thiab rhiab heev.
Qhov txawv ntawm Epitope thiab Paratope yog dab tsi?
Epitope vs Paratope |
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| Ib qho epitope yog thaj chaw tshwj xeeb hauv cov tshuaj tiv thaiv, uas yog qhov chaw tshwj xeeb uas cov tshuaj tiv thaiv khi. | Paratope kuj raug xa mus rau qhov chaw antigen-binding site, yog thaj chaw tshwj xeeb lossis ib feem ntawm cov tshuaj tiv thaiv uas paub txog thiab khi rau thaj tsam epitope ntawm antigen. |
| Lub epitope muaj nyob rau ntawm antigen (ntawm lub cev txawv teb chaws). | thaj chaw Paratope muaj nyob rau ntawm cov tshuaj tiv thaiv ntawm tus tswv. |
| Ntau qhov chaw ntawm kev sib cuam tshuam tuaj yeem nyob hauv thaj chaw epitope. | Ib qhov chaw muaj nyob rau ntawm paratope los cuam tshuam nrog tus epitope. |
| Nyob zoo | |
| High in epitope. | Low in paratope. |
| Type | |
| Nruam, tsis tu ncua, B reactive epitopes thiab T reactive epitopes yog ntau hom epitopes. | Tsis muaj hom tuaj yeem pom hauv paratopes. |
Summary – Epitope vs Paratope
Qhov chaw tseem ceeb ntawm cov tshuaj tiv thaiv thiab cov tshuaj tiv thaiv uas koom nrog cov tshuaj tiv thaiv yog Epitope thiab Paratope. Lub epitope yog qhov chaw nyob rau hauv lub antigen ntawm txawv teb chaws lub cev uas khi rau cov tshuaj tiv thaiv. Lub paratope yog thaj chaw hauv cov tshuaj tiv thaiv uas khi rau cov antigen. Epitopes hauv antigens thiab paratopes hauv cov tshuaj tiv thaiv koom nrog hauv cov tshuaj tiv thaiv antigen-antibody los tsim cov tshuaj tiv thaiv kab mob tiv thaiv txawv teb chaws. Nws yog ib qho tseem ceeb uas yuav tau kawm txog cov cheeb tsam no txhawm rau txheeb xyuas qhov tshwj xeeb ntawm kev tiv thaiv kab mob. Epitope mapping yog cov txheej txheem hloov zuj zus uas ua rau cov kws tshawb nrhiav kom paub meej txog txoj haujlwm thiab cov qauv ntawm epitopes. Yog li cov tshuaj tiv thaiv monoclonal tshwj xeeb tuaj yeem tsim los rau lub hom phiaj ntawm epitope nyob rau hauv vitro mob.
Download the PDF Version of Epitope vs Paratope
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm Epitope thiab Paratope
