Qhov Tseem Ceeb - Protease vs Peptidase
Proteins yog macromolecules. Lawv tsuas yog tsim los ntawm carbon, Hydrogen, Oxygen, thiab Nitrogen. Nws yog ib qho tseem ceeb ntawm cov khoom noj vim nws lub luag haujlwm hauv cov qauv thiab kev ua haujlwm ntawm lub cev. Protein digestion lossis proteolysis pib hauv plab, txawm hais tias feem ntau ntawm kev zom cov protein tshwm sim hauv cov hnyuv me uas siv cov pancreatic enzymes. Cov khoom kawg ntawm kev zom cov protein yog cov amino acids, uas tau nkag mus rau hauv cov hnyuv me thiab thauj los ntawm cov ntshav mus rau lub cev. Protein degradation kuj yog ib txoj hauv kev ua haujlwm nyob rau hauv ib puag ncig kev lag luam. Protein degradation feem ntau yog ua hauv kev lag luam xws li tawv, ntaub plaub, thiab kev lag luam khoom noj. Protein degradation yog ib qho enzyme-catalyzed cov tshuaj tiv thaiv. Li no, tam sim no, cov enzymes no tau tsim tawm thoob ntiaj teb siv cov thev naus laus zis DNA rov ua dua. Ob lub proteolytic enzymes Protease thiab Peptidase muaj kev koom tes hauv protein degradation nyob rau hauv natural phenomena thiab nyob rau hauv industrial scale. Proteases yog ib hom hydrolases, uas koom nrog hauv kev sib cais ntawm peptide daim ntawv cog lus hauv cov proteins thaum peptidases yog ib hom proteases uas muaj peev xwm tshem tawm qhov kawg terminals ntawm peptide saw. Qhov no yog qhov txawv ntawm Protease thiab Peptidase.
YProtease yog dab tsi?
Protease yog ib hom hydrolase uas poob rau hauv qeb ntawm Enzyme commission chav kawm 3 (EC3). Protease koom nrog hauv kev ua kom muaj nucleophile uas yuav tawm tsam cov pa roj carbon ntawm peptide daim ntawv cog lus. Qhov kev tawm tsam nucleophilic no yog ua raws li kev tsim ntawm lub zog siab nruab nrab. Nyob rau hauv thiaj li yuav stabilize qhov nruab nrab no, lub unstable complex yuav degraded mus cuag stability. Qhov kev degradation no yuav ua rau lub cleavage ntawm peptide daim ntawv cog lus ua rau ob feem ntawm peptides. Raws li cov txheej txheem catalytic no, muaj plaub hom proteases: aspartic proteases, cysteine proteases, aspartyl proteases thiab metalloproteases. Txoj kev ntawm nucleophilic nres me ntsis txawv hauv txhua chav enzyme.
Proteases yog siv nyob rau hauv ob lub ntsiab lus tseem ceeb: nyob rau hauv tej yam kev mob ntawm cov protein zom thiab degradation, nyob rau hauv muaj tej yam kev mob los tsim cov khoom lag luam.
Nyob rau hauv cov ntsiab lus ntawm physiology, proteases yog qhov tseem ceeb rau kev zom cov zaub mov protein, hloov pauv cov protein, faib cov cell, cov ntshav txhaws, cov teeb liab hloov, ua cov tshuaj hormones polypeptide, apoptosis thiab lub neej-kuj ntawm ntau yam kab mob. Cov kab mob suav nrog kev rov ua dua ntawm retroviruses.
Daim duab 01: Protease
Kev lag luam kev lag luam ntawm Proteases yog tawv tsim, kev tsim cov ntaub plaub, tsim cov Klenow fragments, peptide synthesis, zom cov protein tsis xav tau thaum lub sij hawm nucleic acid purification, siv cov proteases hauv kev sim kab lis kev cai ntawm tes thiab cov ntaub so ntswg dissociation, kev npaj cov tshuaj tiv thaiv kab mob sib txuas rau Kev tshawb nrhiav, kuaj mob, thiab kho.
Proteases tau faib ntxiv raws li exopeptidases thiab endopeptidases raws li qhov chaw ntawm kev tawm tsam ntawm peptide linkage.
YPeptidase yog dab tsi?
YPeptidase yog hom protease. Cov txheej txheem ntawm kev ua ntawm peptidase zoo ib yam li protease. Peptidase yog tus yam ntxwv ua ib qho exopeptidase thiab koom nrog hauv kev sib txuas ntawm lub davhlau ya nyob twg peptide txuas. Lub davhlau ya nyob twg peptide txuas tuaj yeem yog carboxy terminal kawg lossis amino davhlau ya nyob twg.
Daim duab 02: Peptidase Action
Zoo ib yam li proteases, peptidases kuj muaj ob daim ntawv thov tseem ceeb. Lawv nyob hauv physiology thiab hauv cov ntawv thov kev lag luam.
Dab tsi yog qhov zoo sib xws ntawm Protease thiab Peptidase?
- Ob leeg yog cov enzymes proteolytic.
- Ob leeg yog hydrolase enzymes.
- Ob lub enzymes tuaj yeem tsim los ntawm kev siv thev naus laus zis DNA.
- Ob lub enzymes koom nrog hauv kev tshem cov peptide daim ntawv cog lus ntawm cov protein thiab degrading proteins.
- Ob leeg muaj cov ntawv thov hauv kev lag luam - kev lag luam tawv, kev lag luam wool, kev lag luam khoom noj thiab cov khoom siv DNA recombinant thiab proteomics.
- Nyob rau hauv physiology, proteases thiab peptidases yog siv nyob rau hauv lub digestion txheej txheem.
Qhov txawv ntawm Protease thiab Peptidase yog dab tsi?
Protease vs Peptidase |
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| Proteases yog cov enzymes uas tshem cov peptide daim ntawv cog lus hauv cov proteins. | Peptidases yog ib hom protease uas muaj peev xwm tshem tawm qhov kawg terminals ntawm peptide saw. |
| Action | |
| Protease tuaj yeem yog endopeptidases lossis exopeptidases. | Peptidases yog exopeptidases. |
Summary – Protease vs Peptidase
Proteases thiab peptidases yog cov enzymes proteolytic uas muaj ntau yam haujlwm hauv lub cev. Qhov sib txawv ntawm proteases thiab peptidases yog tias protease tuaj yeem yog endopeptidases lossis exopeptidases thaum peptidases yog exopeptidases. Tam sim no, cov enzymes no yog tsim los ntawm recombinant DNA thev naus laus zis vim nws yuav ua rau muaj txiaj ntsig zoo thiab cov khoom lag luam zoo kawg uas tau txais txiaj ntsig zoo.
Download PDF Version of Protease vs Peptidase
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li cov ntawv sau tseg. Thov download PDF version ntawm no Qhov txawv Ntawm Protease thiab Peptidase
