Qhov Tseem Ceeb Qhov txawv - Exome vs RNA Sequencing
Nucleic acid sequencing yog cov txheej txheem uas txiav txim siab qhov kev txiav txim ntawm nucleotides hauv ib feem ntawm DNA lossis RNA ntawm lub cev. Sequencing yog ib qho tseem ceeb hauv kev txheeb xyuas DNA thiab RNA pleev ntawm lub xov tooj ntawm tes thiab paub qhov txawv ntawm qee cov noob uas code rau cov protein ua haujlwm; Yog li, kev sib sau ua ke tuaj yeem siv los nkag siab txog kev hloov pauv ntawm cov noob no thiab cov kab lus gene. Sanger Sequencing txoj kev los yog cov txheej txheem ntau tshaj Tom ntej no sequencing txoj kev yog cov sequencing txoj kev uas feem ntau siv. Exome sequencing yog qhov ua tiav ntawm cov txheej txheem ntawm exons lossis coding DNA cheeb tsam tam sim no nyob rau hauv ib lub cev whereas RNA sequencing yog cov txheej txheem sequencing ntawm Ribonucleic acids (RNA). Qhov no yog qhov sib txawv tseem ceeb ntawm exome thiab RNA sequencing.
Exome Sequencing yog dab tsi?
Exome yog ib pawg ntawm cov genome uas muaj cov coding noob ntawm ib yam kabmob. Cov noob caj noob ces muaj npe hu ua exons thiab tau muab tso rau hauv mRNA thiab tom qab ntawd muab txhais ua cov amino acid sequences. Thaum lub sijhawm hloov pauv hloov pauv, RNA splicing mechanism hauv eukaryotes tshem tawm cov introns (tsis yog coding cheeb tsam), thiab cov exons tseem nyob. Muaj ob txoj hauv kev uas exome sequencing ua tiav: kev daws teeb meem raws thiab array raws.
Hauv kev daws teeb meem raws li exome sequencing, DNA cov qauv yog fragmented siv cov kev txwv enzymes los yog txhua yam txheej txheem thiab denatured los ntawm tshav kub. Hauv cov txheej txheem no, biotinylated oligonucleotide probes (baits) yog siv los xaiv hybridize nrog cov hom phiaj hauv cheeb tsam hauv genome. Sib nqus streptavidin hlaws dai yog siv rau kev khi cov kauj ruam. Kev khi yog ua raws li los ntawm kev ntxuav cov kauj ruam uas tsis muaj kev sib txuas thiab tsis yog lub hom phiaj raug ntxuav tawm. Cov hom phiaj ua ke tau nthuav dav siv Polymerase Chain cov tshuaj tiv thaiv (PCR) thiab tom qab ntawd tau ua tiav siv Sanger sequencing lossis Next Generation Sequencing techniques.
Daim duab 01: Exome Sequencing
Array raws li txoj kev kuj zoo ib yam li cov kev daws teeb meem, tshwj tsis yog tias DNA tawg tau raug ntes rau hauv micro array, thiab tom qab ntawd kev khi thiab cov kauj ruam ntxuav ua ntej ua ntu zus.
Exome sequencing yog siv rau hauv ntau daim ntawv thov xws li kev kuaj mob caj ces ntawm cov kab mob, hauv kev kho noob, hauv kev txheeb xyuas cov cim caj ces tshiab, hauv kev ua liaj ua teb txhawm rau txheeb xyuas ntau yam txiaj ntsig agronomic thiab hauv cov txheej txheem cog qoob loo.
RNA Sequencing yog dab tsi?
RNA sequencing yog ua raws li cov ntaub ntawv sau tseg, uas yog cov ntawv sau tiav ntawm lub xovtooj. Lub hom phiaj tseem ceeb ntawm RNA sequencing yog txhawm rau txheeb xyuas txhua hom ntawm cov ntawv sau tseg, suav nrog mRNA, tsis yog-coding RNA, thiab RNA me me, los txiav txim siab cov qauv hloov pauv ntawm cov noob thiab txhawm rau txhawm rau ntsuas cov qib qhia ntawm txhua daim ntawv teev lus thaum lub sijhawm txhim kho. Thaum lub sij hawm RNA sequencing, hybridization technologies (uas yog complementary DNA muab tau los ntawm mRNA paub tab) tau pib siv rau sequencing. Tam sim no, qhov tseeb dua thiab cov txheej txheem dhau los ntawm kev tso tawm yog siv rau RNA sequencing.
Daim duab 02: RNA Sequencing
Hauv RNA sequencing, ib qho piv txwv ntawm RNA uas tuaj yeem yog tag nrho RNA lossis fractionated RNA hloov mus rau nws cov DNA ntxiv (cDNA) siv cov ntawv rov qab, thiab lub tsev qiv ntawv cDNA tau npaj. Txhua cDNA fragment txuas rau adapters ntawm ob sab (pair kawg sequencing) los yog ntawm ib sab (ib zaug kawg sequencing). Cov tagged sequences yog sequenced siv Sanger sequencing los yog tiam tom ntej, zoo li exome sequencing.
Dab tsi yog qhov zoo sib xws ntawm Exome thiab RNA Sequencing?
- Cov ntu luv xaiv lossis tag nrho cov DNA / RNA tuaj yeem siv rau Exome lossis RNA sequencing.
- ntu ntu tau khaws cia hauv cov tsev qiv ntawv.
- Sanger sequencing lossis Next Generation sequencing siv tau.
- Ob leeg yog hauv vitro sequencing txoj kev.
- Cov khoom sib txuas tuaj yeem txiav txim siab los ntawm cov cim fluorescent.
Qhov txawv ntawm Exome thiab RNA Sequencing yog dab tsi?
Exome vs RNA Sequencing |
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| Exome sequencing yog qhov ua tiav ntawm cov txheej txheem ntawm exons lossis coding DNA cheeb tsam tam sim no nyob rau hauv lub cev. | RNA sequencing yog hais txog cov txheej txheem sequencing ntawm Ribonucleic acids (RNA); cov ntawv sau tseg. |
| pib qauv | |
| Genomic DNA yog tus qauv pib ntawm exome sequencing. | RNA yog tus qauv pib ntawm RNA sequencing. |
| Qhov no tsuas muaj cov cheeb tsam coding ntawm tag nrho DNA hu ua Exons | This muaj RNA-mRNA / transcriptome. |
| Muaj ob txoj hauv kev tseem ceeb ntawm exome sequencing; daws raws thiab array raws technologies. | RNA sequencing yog ua tiav los ntawm kev npaj ntawm lub tsev qiv ntawv cDNA los ntawm kev rho tawm tag nrho RNA lossis fragmented RNA. |
Summary – Exome vs RNA Sequencing
Exome yog qhov ua tiav ntawm cheeb tsam coding ntawm ib lub cev thiab cov tswv yim koom nrog kev txiav txim siab ntawm kev txiav txim siab nucleotide ntawm Exome yog hu ua exome sequencing. RNA sequencing yog cov txheej txheem koom nrog hauv kev txiav txim siab ntawm nucleotide kev txiav txim ntawm RNA ntawm lub cev. Qhov no yog qhov txawv ntawm exome thiab RNA sequencing.
Download PDF Version of Exome vs RNA Sequencing
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm Exome thiab RNA Sequencing
