Qhov Tseem Ceeb – Nutrient Agar vs Nutrient Broth
Cov kab mob kis tau loj hlob ntawm kab lis kev cai xov xwm. Muaj ntau hom xov xwm xws li cov khoom siv xov xwm, cov xov xwm semisolid thiab cov xov xwm ua kua. Cov xov xwm no tau npaj raws li lub hom phiaj ntawm kev loj hlob kab mob. Cov zaub mov nruab nrab yog npaj rau cov kab mob loj hlob hauv cov chaw sim. Nws muaj ntau yam khoom xyaw nrog rau nitrogen qhov chaw, proteins qhov chaw, dej, thiab NaCl. Agar yog ib yam khoom uas siv los ua tus neeg sawv cev khov kho hauv kev npaj xov xwm. Nutrient kua nruab nrab los yog cov khoom nruab nrab muaj peev xwm npaj tau nrog lossis tsis ntxiv agar. Yog tias agar ntxiv rau qhov nruab nrab nutrient, nws yog hu ua agar nruab nrab. Yog hais tias agar tsis ntxiv rau qhov nruab nrab nutrient, ces nws yog lub npe hu ua ib tug as-ham kua nruab nrab los yog ib tug nutrient broth. Li no, qhov sib txawv tseem ceeb ntawm nutrient agar thiab nutrient broth yog cov as-ham muaj agar thaum nutrient broth tsis muaj agar.
Nutrient Agar yog dab tsi?
Nutrient agar yog ib lub hom phiaj dav dav uas siv los cog cov kab mob. Nws txhawb kev loj hlob ntawm cov kab mob uas tsis yog-fastidious. Nws muaj ntau yam khoom xyaw xws li nitrogen qhov chaw, vitamin qhov chaw, carbohydrates, thiab ib tug solidifying agent.
Kev npaj ntawm Nutrient Agar
Cov khoom xyaw tseem ceeb yog ntsuas thiab yaj hauv dej distilled. Tom qab ntawd cov pH ntawm nruab nrab yog hloov kho siv HCl lossis NaOH. Thaum lub pH raug kho, lub ntim yog topped mus rau qhov tseeb ntim. Agar yog ntxiv rau qhov npaj ncua kev kawm ntawv thiab autoclaved. Thaum nruab nrab yog kom tsis muaj menyuam, nws tuaj yeem muab nchuav rau hauv cov kab mob uas tsis muaj kab mob rau cov kab mob loj hlob hauv cov chaw kuaj mob.
Ntawm agar nruab nrab muaj pes tsawg leeg ib liter yog raws li nram no.
| YPeptone | 5 g |
| Yeast Extract Yeast | 1.5 g |
| Neeg Neeg Extract | 1.5 g |
| Nacl | 5g |
| Agar | 15g |
| pH | 7.4 ± 0.2 |
Nutrient agar yog ib txwm npaj nyob rau hauv microbiology chaw soj nstuam kom loj hlob cov kab mob rau kev sib cais, tus yam ntxwv, kev txheeb xyuas, DNA cais, thiab lwm yam
Daim duab 01: Nutrient Agar Plate
Nutrient Broth yog dab tsi?
Nutrient broth yog cov kua nruab nrab uas npaj kom loj hlob ntau yam kab mob hauv cov chaw sim. Cov muaj pes tsawg leeg ntawm cov as-ham yog tib yam li cov as-ham tshwj tsis yog ntxiv rau agar. Agar tsis siv vim nws yog tus neeg sawv cev khov kho. Ob qho khoom noj khoom haus agar thiab nutrient broth yog amber xim tom qab kev npaj. Nutrient broth yog yooj yim dua nutrient agar raws li feem ntau cov kab mob loj hlob nyob rau hauv kua media. Nutrient broth yog siv los kawm txog cov kev xav tau ntawm cov pa oxygen sib txawv ntawm cov kab mob. Piv txwv li, tsis yog txhua tus kab mob xav tau oxygen; Qee cov kab mob raug lom los ntawm oxygen thaum qee qhov xav tau cov pa me me xwb. Cov kev xav tau no tuaj yeem nkag siab yooj yim thaum lawv loj hlob hauv cov khoom noj muaj txiaj ntsig zoo. Raws li cov pa oxygen xav tau los ntawm cov kab mob, cov kab mob tuaj yeem muab faib ua ntau pawg xws li cov kab mob aerobic, obligate anaerobic bacteria, aerotolerant bacteria, microaerophilic bacteria thiab facultative aerobic bacteria.
Daim duab 02: Cov khoom noj khoom haus kua nruab nrab hauv cov raj kuaj
Qhov txawv ntawm Nutrient Agar thiab Nutrient Broth yog dab tsi?
Nutrient Agar vs Nutrient Broth |
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| Nutrient Agar yog ib qho khoom nruab nrab uas npaj kom loj hlob ntau yam kab mob | Nutrient Broth yog kua nruab nrab uas npaj kom loj hlob ntau yam kab mob. |
| Nutrient Agar muaj peptone, poov xab extract, nqaij nyuj extract, NaCl, agar, thiab dej. | Nutrient Broth muaj peptone, poov xab extract, nqaij nyuj extract, NaCl, thiab dej. |
| Nyob Agar | |
| Nutrient Agar muaj agar rau solidification. | Nutrient Broth tsis muaj agar. |
| khoom | |
| Nutrient agar is a solid medium. | Nutrient Broth yog kua nruab nrab. |
| khob iav | |
| Nutrient Agar yog nchuav rau hauv daim phiaj petri, lub raj mis kab lis kev cai, raj kuaj, thiab lwm yam. | Nutrient Broth yog poured rau hauv kab lis kev cai fwj, xeem tub, thiab lwm yam. |
Summary – Nutrient Agar vs Nutrient Broth
kab lis kev cai yog npaj los cog cov kab mob. Lawv tuaj yeem yog cov khoom siv los yog kua dej. Nutrient agar thiab nutrient broth yog ob hom kev tshaj tawm uas tau npaj rau tib txoj kev thiab muaj ntau dua los yog tsawg dua tib yam. Lub ntsiab sib txawv ntawm nutrient agar thiab nutrient broth yog ntxiv ntawm agar. Ob qho kev tshaj tawm yog siv los loj hlob ntau yam kab mob uas tsis yog yoo mov hauv chav kuaj.
Download PDF Version of Nutrient Agar vs Nutrient Broth
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li cov ntawv sau tseg. Thov download PDF version ntawm no Qhov txawv Ntawm Nutrient Agar thiab Nutrient Broth.
