Qhov Tseem Ceeb - HFR vs F + Hom
Cov kab mob sib txuas yog ib txoj hauv kev sib deev hauv cov kab mob thiab suav tias yog ib hom kab rov tav hloov mus rau hauv cov kab mob. Nws muaj peev xwm ua tau ntawm ob tus kab mob uas ib tus kab mob muaj cov kab mob fertility lossis F plasmid thiab cov kab mob thib ob tsis muaj F plasmid. Thaum cov kab mob sib txuas, F plasmids feem ntau kis mus rau tus neeg tau txais kab mob, tsis yog tag nrho cov chromosome. Cov kab mob uas muaj F plasmids hu ua F + hom lossis cov neeg pub dawb. Lawv muaj peev xwm tsim kev sib deev pili thiab hloov plasmids mus rau lwm cov kab mob uas tau txais lawv. F plasmid yog dawb hauv cytoplasm. Qee zaum, F plasmid koom ua ke rau hauv cov kab mob chromosome thiab tsim cov DNA recombinant. Cov kab mob uas muaj F plasmid sib xyaw ua ke rau hauv lawv cov chromosomes yog hu ua high zaus recombinant hom lossis Hfr hom. Qhov sib txawv tseem ceeb ntawm F + hom kab mob thiab Hfr yog F + hom kab mob muaj F plasmids hauv cytoplasm ywj pheej yam tsis muaj kev koom ua ke rau hauv cov kab mob chromosomes thaum Hfr hom muaj F plasmids koom ua ke rau lawv cov chromosomes.
F + Hom kab mob dab tsi?
Qee cov kab mob muaj F plasmids ntxiv rau lawv cov chromosomes. Cov hom no yog hu ua F + hom. Lawv ua raws li cov neeg pub dawb lossis cov txiv neej hauv cov kab mob sib txuas. Bacterial conjugation yog ib txoj kev sib deev kev sib deev uas pom los ntawm cov kab mob uas pab txhawb cov kab mob kab rov tav hloov ntawm cov kab mob. F plasmids tuaj yeem rov ua dua ntawm nws tus kheej thiab muaj cov txheej txheem fertility coding noob. Li no cov extrachromosomal DNA (plasmids) yog hu ua F plasmids vim yog F yam lossis fertility factor. Fertility factor coding noob yog qhov tseem ceeb rau kev hloov pauv lossis kev sib txuas. Cov kab mob kab mob uas tau txais F plasmids los ntawm F + hom kab mob hu ua F- hom kab mob los yog cov neeg tau txais hom kab mob los yog poj niam. F + hom tuaj yeem pub lawv cov khoom siv caj ces lossis cov DNA ntxiv rau lwm tus kab mob.
Cov kab mob sib kis pib nrog kev tsim tawm ntawm kev sib deev pili los ntawm F + hom kab mob mus rau F- kab mob. Kev sib deev pilus pab txhawb lub cell mus rau kev sib txuas lus ntawm tes thiab kev sib cuag los ntawm kev tsim lub raj txuas. Qhov kev tsim no yog tswj hwm los ntawm cov noob qoob loo uas yug los ntawm F + hom. F + replicates nws F plasmid thiab ua ib daim qauv ntawm nws mus rau hauv F- hom. Cov theej F plasmid raug xa mus rau F- hom ntawm lub raj txuas. Thaum nws hloov mus, conjugation tube dissociates. Tus neeg txais kev lim ntshav dhau los ua F +. Thaum lub sij hawm cov kab mob conjugation, tsuas yog F plasmid kis los ntawm F + hom mus rau F- hom; cov kab mob chromosome tsis hloov.
Daim duab 01: F + Lim thiab F- Lim
HFR Strains yog dab tsi?
Cov kab mob kab mob uas muaj F plasmid koom ua ke rau hauv cov chromosomes hu ua high-frequency recombination strains or Hfr strains. Hauv Hfr hom, F plasmid tsis muaj nyob hauv cytoplasm. F plasmid sib txuas nrog cov kab mob chromosome thiab muaj nyob ua ib chav. Cov DNA recombined no yog hu ua high-frequency DNA lossis Hfr DNA. Hauv lwm lo lus, nws yog kab mob kab mob uas muaj Hfr DNA ua Hfr hom. Txij li Hfr strain muaj F plasmid lossis fertility yam nws tuaj yeem ua tus neeg pub dawb lossis txiv neej kab mob hauv cov kab mob sib txuas. Cov kab mob Hfr no sim hloov tag nrho DNA lossis ib feem loj ntawm DNA mus rau tus neeg tau txais kab mob los ntawm tus choj mating. Qee qhov chaw ntawm cov kab mob chromosome lossis tag nrho cov chromosome kuj tuaj yeem luam tawm thiab xa mus rau tus neeg tau txais kab mob thaum Hfr strain koom nrog kev sib txuas. Cov kab mob Hfr zoo li no muaj txiaj ntsig zoo hauv kev kawm txog kev sib txuas ntawm cov noob caj noob ces thiab rov ua dua. Yog li, cov kws tshawb fawb molecular biologists thiab geneticists siv Hfr hom kab mob (feem ntau E. coli) los kawm txog kev sib txuas ntawm caj ces thiab qhia cov chromosome.
High-frequency recombination tshwm sim thaum tus neeg tau txais cov kab mob tau txais peb hom DNA tom qab mating nrog Hfr strain los ntawm cov kab mob sib txuas. Peb hom no yog, nws tus kheej chromosomal DNA, F plasmid DNA thiab qee qhov ntawm tus pub dawb chromosomal DNA. Vim li no, cov kab mob no muaj npe hu ua Hfr hom. HFr hom kuj tseem tuaj yeem txhais tau tias yog derivatives ntawm F + hom.
F plasmids tuaj yeem koom ua ke rau hauv cov kab mob chromosome thiab disintegrate rov qab los ntawm tus tswv chromosome. Thaum lub sij hawm tawg, F plasmid tuaj yeem xaiv qee cov noob nyob ze nws los ntawm tus tswv chromosome. Hfr kab mob uas cuam tshuam nrog qee tus tswv tsev noob nyob ib sab ntawm F plasmid kev koom ua ke qhov chaw hu ua F' hom.
Daim duab 02: Hfr Strain
Qhov txawv ntawm HFR thiab F + Hom kab mob yog dab tsi?
HFR vs F + Hom |
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| HFr hom kab mob yog cov kab mob uas muaj Hfr DNA lossis F plasmid DNA koom ua ke hauv cov kab mob chromosomes. | Cov kab mob kab mob uas muaj F plasmids hu ua F + hom. F plasmids muaj fertility factor coding genes. |
| Fertility Factor | |
| Lub fertility plasmid yog kev koom ua ke rau hauv lub cell chromosomal DNA hauv Hfr hlwb. | Fertility plasmid yog ywj siab ntawm chromosome hauv F + hlwb |
| Efficiency | |
| Hfr yog cov pub dawb zoo heev. | F+ hlwb tsawg dua piv rau Hfr hom. |
Summary – Hfr vs F+ Strains
Cov kab mob kab mob uas muaj F plasmids yog tus cwj pwm li F + hom. F plasmids muaj qhov fertility factor lossis F yam tseem ceeb rau kev sib txuas ntawm cov kab mob. Cov kab mob no muaj peev xwm hloov lawv cov F plasmid mus rau cov kab mob uas tsis muaj F plasmids. Thaum cov F plasmids nkag mus rau hauv tus neeg tau txais kab mob, nws tuaj yeem nyob ntawm nws tus kheej lossis nws tuaj yeem koom nrog cov kab mob chromosome. Integrated F plasmid DNA thiab chromosomal DNA yog hu ua Hfr DNA. Cov kab mob kab mob uas dais Hfr DNA lossis F plasmid DNA koom ua ke hauv cov kab mob chromosomes hu ua HFr hom. Qhov no yog qhov txawv ntawm F + thiab Hfr hom.
Download PDF Version of HRF vs F+ Strains
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li cov ntawv sau tseg. Thov download PDF version ntawm no Qhov txawv Ntawm HFR thiab F + Hom
