Qhov Tseem Ceeb - Hloov pauv vs Kev Hloov pauv
Hauv biotechnology thiab genetic engineering, cov noob txawv teb chaws tau nkag mus rau hauv genomes ntawm cov kab mob nrog lub hom phiaj ntawm kev txhim kho cov yam ntxwv ntawm cov kab mob. Muaj cov txheej txheem ntawm lub cev, tshuaj lom neeg thiab tshuaj lom neeg muaj los qhia cov DNA txawv teb chaws rau hauv cov tswv tsev. Transfection thiab transduction yog ob hom kev hloov cov noob uas siv hauv molecular biology. Qhov sib txawv tseem ceeb ntawm kev hloov pauv thiab hloov pauv yog tias kev hloov pauv yog kev hloov pauv cov noob tsis yog kab mob uas siv tshuaj thiab lub cev txoj hauv kev thaum hloov pauv yog kab mob kis kab mob. Kev hloov pauv tau yooj yim los ntawm cov khoom siv tshuaj lom neeg lossis cov khoom tsis yog tshuaj lom neeg thaum kev hloov pauv los ntawm cov kab mob kis.
Kev hloov pauv yog dab tsi?
Kev hloov pauv yog ib txoj hauv kev hloov cov noob uas cuam tshuam nrog cov vectors tsis yog kab mob rau kev qhia txog noob. Kev hloov pauv tuaj yeem ua tiav siv cov khoom siv tshuaj xws li calcium phosphate, cationic polymers, liposomes lossis siv cov txheej txheem tsis siv tshuaj xws li electroporation, microprojectile bombardment, thiab lwm yam. Lub hauv paus tseem ceeb tom qab kev hloov pauv yog nce permeability ntawm cov cell membranes tso cai nkag mus rau txawv teb chaws DNA. hauv cov hlwb. Nws yog ua tiav los ntawm kev qhib cov pores nyob rau hauv lub cell membrane.
Liposomes yog cov hlwv me me uas muaj daim nyias nyias ua los ntawm phospholipid molecules zoo ib yam li cov cell membrane. Lawv tuaj yeem yooj yim merged nrog cell membranes vim nws muaj pes tsawg leeg. Liposomes yog siv los xa cov DNA txawv teb chaws rau hauv cov hlwb vim qhov yooj yim ntawm kev sib koom ua ke nrog cov cell membranes. Microprojectile bombardment yog lwm txoj kev hloov pauv uas cuam tshuam nrog cov kub ceev ceev lossis cov khoom tungsten coated nrog txawv teb chaws DNA xa mus rau hauv cov hlwb. Electroporation siv hluav taws xob teb los qhib qhov pores ib ntus thiab ua kom cov cell membrane permeability nce mus rau txawv teb chaws DNA. Calcium phosphate nanoparticles kuj tseem siv rau hauv kev hloov pauv mus xa cov DNA txawv teb chaws rau hauv cov hlwb eukaryotic.
Daim duab 01: Kev hloov pauv
Kev hloov pauv yog dab tsi?
Viruses yog cov kab mob hauv lub cev uas muaj lub peev xwm los ntxig lawv cov khoom siv caj ces rau hauv cov hlwb uas tsis muaj kev txhawb nqa los ntawm kev kis kab mob. Cov txheej txheem biotechnological tau tshawb nrhiav qhov peev xwm no rau kev hloov pauv DNA txawv teb chaws nrog cov noob tshwj xeeb rau hauv cov kab mob. Txoj kev no hu ua transduction. Yog li, kev hloov pauv tuaj yeem txhais tau tias yog cov txheej txheem uas siv tus kab mob lossis tus kab mob kis mus rau kev qhia txog DNA txawv teb chaws rau hauv cov tswv tsev. Ntawm cov kab mob no, bacteriophages yog nrov hauv kev hloov pauv. Bacteriophages yog ib pawg ntawm cov kab mob uas kis kab mob. Lawv tuaj yeem tsim cov kab mob caj ces los ntawm ib tus kab mob mus rau lwm tus kab mob los ntawm kev kis kab mob. T4 thiab Phage lamda yog qhov nrov rau cov txheej txheem hloov cov noob.
Kev hloov pauv yog hom kev hloov pauv ntawm cov khoom siv caj ces ntawm cov kab mob. Nws tshwm sim ntawm lytic lossis lysogenic cycles. Hauv lub voj voog lytic, cov kab mob hlwb cuam tshuam thiab tso cov phages tshiab nrog cov genomes sib xyaw rau sab nraud. Nyob rau hauv lub voj voog lysogenic, phage genetic khoom integrates rau hauv cov kab mob chromosomes thiab ua dormant rau ob peb tiam.
Daim duab 02: Kev hloov pauv dav dav
Dab tsi yog qhov sib txawv ntawm Kev Hloov Kho thiab Kev Hloov Pauv?
Kev hloov pauv hloov pauv |
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| Kev hloov pauv yog ib qho cuab yeej ntawm kev hloov cov noob uas siv cov tshuaj lom neeg lossis cov khoom tsis muaj tshuaj lom neeg hauv cov hlwb eukaryotic. | Kev hloov pauv yog ib qho cuab yeej ntawm kev hloov cov noob uas siv tus kab mob lossis kab mob kab mob feem ntau ntawm cov kab mob. |
| Nws yog ua los ntawm kev qhib lub qhov hws nyob rau hauv lub cell membranes. | Tus kab mob kis mus rau tus tswv tsev cell thiab ntxig nws cov khoom siv caj ces thiab rov ua dua ntawm DNA rau hauv cov kab mob genome. |
| Nature of Method | |
| Kev hloov pauv tuaj yeem ua tau siv tshuaj thiab lub cev. | Kev hloov pauv yog ib txoj hauv kev lom neeg ntawm kev hloov cov noob. |
| Ntau Hom | |
| Liposome transfection, electroporation, microprojectile bombardment yog cov piv txwv ntawm kev hloov pauv. | Generalized thiab tshwj xeeb yog ob hom kev hloov pauv. |
Summary – Transfection vs Transduction
Kev hloov pauv thiab hloov pauv yog cov cuab yeej siv hauv Biotechnology los qhia cov noob txawv teb chaws rau hauv cov tswv tsev. Kev hloov pauv yog ua los ntawm kev siv cov tshuab tsis muaj kab mob xws li tshuaj lom neeg thiab cov neeg nqa khoom. Kev hloov pauv yog ib qho cuab yeej uas qhia txog cov noob txawv teb chaws lossis DNA rau hauv cov hlwb uas siv cov kab mob raws li kab mob. Thaum lub sij hawm transfection, DNA yog txhob txwm tshaj tawm mus rau hauv lub host cell thaum transduction yog ib txwm ua los ntawm cov kab mob. Qhov no yog qhov sib txawv ntawm transfection thiab transduction. Ob txheej txheem tseem ceeb hauv kev kho noob.
