Qhov Tseem Ceeb - DNA vs DNAse
DNA yog ib qho nucleic acid uas muaj nyob hauv cov nucleus ntawm cov hlwb. Nws nqa cov ntaub ntawv keeb kwm ntawm cov hlwb uas yog qhov tseem ceeb rau kev loj hlob, kev loj hlob, metabolism thiab kev tsim tawm ntawm cov kab mob. DNA molecule yog tsim los ntawm deoxyribonucleotides teem nyob rau hauv ntev chains. DNAse yog ib qho enzyme uas muaj peev xwm tshem tawm phosphodiester bonds ntawm nucleotides ntawm DNA thiab ua rau degradation ntawm DNA. Nws yog tsim los ntawm cov amino acids. Qhov sib txawv tseem ceeb ntawm DNA thiab DNAse yog DNA yog ib qho nucleic acid uas dais cov ntaub ntawv caj ces ntawm cov kab mob thaum DNAse yog ib qho enzyme uas degrades DNA hauv cell.
DNA yog dab tsi?
Deoxyribonucleic acid (DNA) yog ib hom ntawm nucleic acid pom nyob rau hauv lub nucleus ntawm cov hlwb. DNA yog cov khoom siv caj ces ntawm feem ntau cov kab mob. Nws yog tsim los ntawm deoxyribonucleotide monomers. Ib tug deoxyribonucleotide yog tsim los ntawm peb lub ntsiab Cheebtsam: ib tug nitrogenous puag, deoxyribose qab zib, thiab ib pawg phosphate. Muaj plaub hom nitrogenous hauv paus muaj nyob hauv DNA. Lawv yog adenine (A), guanine (G), cytosine (C), thiab thymine (T). Deoxyribonucleotides txuas nrog ib leeg los ntawm phosphodiester bonds tsim ntawm 5 'phosphate pawg thiab 3' OH pawg ntawm cov nucleotides uas nyob ib sab. Qhov kev txiav txim ntawm lub hauv paus ib ntus dais cov ntaub ntawv caj ces uas dhau mus rau tiam tom ntej los ntawm DNA replication.
DNA muaj nyob hauv ob lub helix. Ob txoj hlua ntawm polynucleotides txuas rau ib leeg los ntawm hydrogen bonds ntawm cov hauv paus ntxiv (A nrog T thiab C nrog G). Cov piam thaj molecule thiab phosphate pawg yog cov pob txha ntawm DNA molecule thaum nitrogenous bases yog tsim nyob rau hauv nruab nrab ntawm lub helix. DNA molecule (ob chav helix) zoo li tus ntaiv rau qee qhov raws li qhia hauv daim duab 01.
Daim duab 01: DNA Ob Chav Helix
YDNAse yog dab tsi?
Deoxyribonuclease (DNAse) yog ib qho nuclease enzyme lub luag haujlwm rau kev degradation ntawm DNA. Nws hydrolyzes 3'-5' phosphodiester bonds ntawm nucleotides thiab cais nucleotides. Qhov no yog ib qho tseem ceeb enzyme rau recombinant DNA thev naus laus zis los txiav cov kab mob tshwj xeeb lossis cov noob rau kev sib txuas thiab cloning.
DNAse feem ntau yog ob hom: DNAse I thiab DNAse II. Qee qhov yog endonucleases uas hydrolyze tshuaj bonds hauv DNA molecule thaum qee cov DNAses yog exonucleases uas tshem tawm nucleotides los ntawm qhov kawg ntawm DNA molecule.
DNAse yog siv thaum lub sij hawm purification ntawm RNA kom tshem tawm cov kab mob DNA los ntawm degradation. DNAse kuj tseem siv tau los ua cov khoom me me me me uas hnyav ntawm cov genomic DNA rau hneev taw, DNA nick txhais lus, tshem tawm cov qauv DNA tom qab hauv vitro transcription thiab lwm yam.
Daim duab 02: DNAse I Structure
DNA thiab DNA sib txawv li cas?
DNA vs DNAse |
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| DNA yog nucleic acid. | DNAse yog protein. |
| Major Functions | |
| DNA yog qhov chaw khaws cov ntaub ntawv keeb kwm ntawm yuav luag txhua yam kabmob. | DNAse yog ib qho enzyme muaj peev xwm ntawm hydrolyzing phosphodiester bonds ntawm nucleotides hauv DNA. |
| Nws yog tsim los ntawm deoxyribonucleotides. Li no, nws yog polynucleotide. | Nws yog tsim los ntawm cov amino acids. Li no, nws yog polypeptide. |
| DNA muaj nyob hauv cov nucleus, mitochondria, thiab chloroplasts ntawm cov hlwb. | DNA muaj nyob hauv cytoplasm ntawm cov hlwb. |
| Kev xa mus rau tiam neeg ua tiav | |
| Nws muaj peev xwm hla cov ntaub ntawv ntawm ib tiam mus rau lwm tiam. | Nws tsis cuam tshuam nrog kev xeeb tub. |
| Replicating Ability | |
| DNA tuaj yeem rov ua dua los ua ib daim qauv zoo tib yam. | DNAse tsis tuaj yeem rov ua dua. |
| Synthesis | |
| DNA yog tsim los ntawm DNA replication thaum lub sij hawm cell division. | DNAse yog tsim los ntawm ribosomes |
| Siv hauv Recombinant DNA Technology | |
| DNA nws tus kheej tau raug rov ua dua nrog vector DNA hauv kev siv thev naus laus zis DNA. | Qhov no yog siv hauv kev siv thev naus laus zis los txiav DNA. Nws yog lub cuab yeej molecular muaj zog. |
Summary – DNA vs DNAse
DNA yog ib qho nucleic acid uas muaj deoxyribonucleotides. Nws muaj cov ntaub ntawv keeb kwm ntawm lub cev thiab pom nyob rau hauv lub nucleus. DNA muaj nyob rau hauv ob daim ntawv helix thiab muaj ib tug tshwj xeeb nucleotide sequence. DNA yog npaj rau hauv me me subsets hu ua noob. Cov noob yog encoded rau cov proteins thiab lwm yam khoom tseem ceeb rau cov kab mob. DNAse yog ib qho enzyme lub luag hauj lwm rau cleaving phosphodiester bonds ntawm nucleotides ntawm DNA. Nws yog tsim los ntawm cov amino acids thiab pom nyob rau hauv cytoplasm ntawm lub cell. Qhov no yog qhov sib txawv ntawm DNA thiab DNAse.
