Qhov txawv ntawm Benzonase thiab DNase

2024 Tus sau: Alex Aldridge | [email protected]. Kawg hloov kho: 2023-12-17 13:40

Qhov Tseem Ceeb Qhov txawv - Benzonase vs DNase

Kev txo qis ntawm nucleic acids yog qhov tseem ceeb rau ntau cov txheej txheem biology molecular. Nws yog dav siv nyob rau hauv recombinant DNA thev naus laus zis los tshem tawm cov tsis xav tau ntawm DNA thiab RNA. Nucleic acid degrading enzymes raug xa mus rau Nucleases, thiab lawv tuaj yeem ua ntau hom raws li qhov yuav tsum tau ua. Nucleases uas degrade DNA yog hu ua DNases whereas cov uas degrade RNA paub RNases. Cov enzymes no feem ntau yog siv nyob rau hauv vitro thwmsim qhov twg hauv vitro molecular kuaj tau ua los cais cov DNA ntshiab, RNA lossis cov proteins. Benzonases yog ib hom nucleases uas degrade ob DNA thiab RNA whereas DNases degrade tsuas yog DNA. Qhov no yog qhov sib txawv tseem ceeb ntawm Benzonase thiab DNase.

Benzonase yog dab tsi?

Benzonase yog ib qho kev tsim kho caj ces endonuclease los ntawm Serratia marcescens. Qhov no enzyme yog tsim nyob rau hauv E. coli hosts nyob rau hauv industrial scale. Benzonase muaj peev xwm ntawm cleaving ob-stranded DNA, linear DNA, ncig DNA thiab ib leeg-stranded RNA. Yog li Benzonase yog kev lag luam tseem ceeb. Benzonase enzyme yog cov protein dimer uas muaj 245 zoo tib yam amino acids, ~ 30 kDa subunits nrog ob qhov tseem ceeb disulfide bonds. Benzonase cleaves nucleic acids ntawm nws 5' kawg thiab ua rau tawg tawg nrog dawb 5'ends. Benzonase tuaj yeem tshem cov nucleic acids ntawm txhua ntu tab sis nyiam GC cov cheeb tsam nplua nuj.

Benzonase khaws cia ntawm -20 ^{0 C. Qhov zoo tshaj pH rau kev ua enzyme pom tau tias yog 8.0 - 9.2. Cov ntawv thov ntawm Benzonase suav nrog cov qauv kev npaj rau cov protein 2D gel electrophoresis qhov twg Benzonase tshem tawm cov kab ke nucleic acids thiab tshem tawm cov kab mob nucleic acid los ntawm kev npaj cov protein. Nws kuj yog siv los txo cov viscosity ntawm cov protein rho tawm thiab tiv thaiv clumping ntawm cov hlwb hauv cov cell sib tov.}

DNase yog dab tsi?

DNase yog ib qho nuclease, hydrolytic enzyme uas tsuas muaj peev xwm tshem tawm ob txoj hlua DNA. Muaj ob hom DNases tseem ceeb: DNase I thiab DNase II. DNase Kuv koom nrog hauv kev tshem tawm ob-stranded DNA los tsim polynucleotides nrog 5' dawb xaus. DNase II tau koom nrog hauv kev tshem tawm ob-kab DNA los tsim cov polynucleotide strands nrog 3 'dawb kawg lossis overhangs.

DNase I

DNase I ua haujlwm ntawm qhov zoo tshaj pH ntawm 7.0 - 8.0. Kev ua enzyme nyob ntawm ntau cov ionic cofactors uas suav nrog, Ca²⁺, Mg²⁺ lossis Mn^{2+Cov haujlwm ntawm Mg}2+ ^{thiab Mn}2+ ^{txiav txim siab txoj haujlwm ntawm DNase I. Nyob ntawm Mg} 2+^{, DNase kuv cleaves txhua strand ntawm dsDNA ntawm nws tus kheej. Qhov no tshwm sim nyob rau hauv ib tug random yam. Nyob rau hauv sib piv, nyob rau hauv lub xub ntiag ntawm Mn} 2+, ^{cov enzyme cleaves ob DNA strands ntawm kwv yees li ntawm tib qhov chaw. Qhov kev sib cais no yuav ua rau tsim ob hom DNA tawg; ib hom nrog blunt kawg thiab lwm hom nrog ib los yog ob nucleotide overhangs.}

Daim duab 02: DNase

DNase II

DNase II ua haujlwm ntawm qhov zoo tshaj pH ntawm 4.5-5.0 thiab divalent hlau ions yog xav tau rau nws cov dej num, zoo ib yam li DNase I. Lub tshuab ntawm DNase II paub tias muaj peb theem tseem ceeb.

1. Ntau qhov kev sib cais ib leeg yog ua rau hauv DNA qaum.
2. Acid soluble nucleotides thiab oligonucleotides raug tsim.
3. Non-linear hyperchromic hloov pauv tshwm sim nyob rau theem kawg.

Lub ntsiab inhibitors ntawm DNase enzyme muaj xws li hlau chelators, hloov hlau thiab tshuaj xws li Sodium dodecyl sulfate thiab β – mercaptoethanol.

Cov ntawv thov tseem ceeb ntawm DNase suav nrog kev npaj cov DNA tsis pub RNA rho tawm thiab cov protein rho tawm, thiab tshem tawm cov qauv DNA thaum lub sij hawm hauv vitro transcription thwmsim.

Dab tsi zoo sib xws ntawm Benzonase thiab DNase?

• Ob leeg yog hydrolytic enzymes.
• ob leeg yog nucleases.
• Ob leeg koom nrog los ntawm cleaving phosphodiester bonds ntawm nucleic acids.
• Ob leeg xav tau qhov zoo tshaj pH thiab qhov kub thiab txias kom tswj tau cov haujlwm ntawm cov enzyme.
• Inhibitors ntawm enzymes suav nrog cov tshuaj chelating, cov hlau hloov pauv, thiab tshuaj ntxuav tes.
• Daim ntawv thov yog tsom rau kom tau txais cov txiaj ntsig zoo ntawm DNA, RNA, thiab cov proteins.
• Ob lub enzymes tuaj yeem tsim los ntawm kev tsim kho caj ces.

Qhov txawv ntawm Benzonase thiab DNase yog dab tsi?

Benzonase vs DNase
Benzonase yog ib qho enzyme uas muaj peev xwm tshem tawm ob txoj kab DNA, kab DNA, ncig DNA, thiab RNA.	DNase yog ib qho enzyme uas muaj peev xwm tshem tawm ob-stranded DNA.
substrate rau Enzyme
Ob DNA thiab RNA yog substrates rau benzonase.	DNA yog substrate rau DNase.
qauv
zoo pH ntau ntawm Benzonase yog 7.0 -8.0	Qhov zoo tshaj pH ntawm DNase I yog 7.0 - 8.0 thiab DNase II yog 4.5 - 5.0.

Summary – Benzonase vs DNase

Nuclease enzymes tau siv dav hauv cov txheej txheem kev sim sib txawv hais txog molecular biology thiab genetic engineering. Benzonase thiab DNase yog ob hom nucleases. Benzonase koom nrog hauv kev degrading ob DNA thiab RNA thaum DNase koom nrog hauv kev tshem tawm ob txoj hlua DNA. Qhov no yog qhov sib txawv ntawm benzonase thiab DNase. Tam sim no, ob hom nuclease no yog tsim los ntawm kev sib txuas DNA thev naus laus zis uas ua rau cov enzymes zoo dua uas tau ua kom zoo rau ntau lawm.

Download PDF Version of Benzonase vs DNase

Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv Ntawm Benzonase thiab DNase