Qhov Tseem Ceeb Qhov txawv - HPLC vs LCMS
Cia peb xub saib lub ntsiab lus ntawm HPLC thiab LCMS ua ntej txheeb xyuas qhov txawv ntawm HPLC thiab LCMS. Chromatography yog cov txheej txheem sib cais hauv kev tshuaj xyuas tshuaj uas cov qauv sib xyaw tau sib cais thaum dhau los ntawm qhov nruab nrab chromatographic. Nws kuj tseem muaj kev cuam tshuam nrog cov qauv, theem nyob ruaj khov, thiab theem mobile. HPLC stands rau High Performance Liquid Chromatography, thiab nws yog siv raws li ib tug kua chromatography txoj kev nyob rau hauv analytical chemistry. Kev sib xyaw ua ke ntawm Liquid Chromatography thiab Mass Spectroscopy (LCMS) tau tsim los rau kev txheeb xyuas ntau ntawm cov biomolecules uas tau xaiv thiab nws yog ib qho kev soj ntsuam zoo, raug, thiab cov txheej txheem tshwj xeeb piv rau HPLC. Qhov no yog qhov sib txawv tseem ceeb ntawm HPLC thiab LCMC. Kab lus no yuav qhia koj txog HPLC thiab LCMC uas cuam tshuam nrog tshuaj ntsuam xyuas thiab sib tham txog qhov sib txawv ntawm HPLC thiab LCMS.
HPLC yog dab tsi?
High-performance kua chromatography (HPLC) yog ib qho kev sib cais nrov hauv kev tshuaj ntsuam chemistry. Nws yog tsuas yog siv los cais cov Cheebtsam, txheeb xyuas thiab ntsuas txhua qhov sib xyaw ua ke. Yav dhau los, txoj kev no tau raug hu ua high-pressure kua chromatography vim tias nws nyob ntawm cov twj tso kua mis kom ntws cov kua hnyav hnyav uas muaj cov qauv sib xyaw los ntawm ib kem ntim nrog cov khoom adsorbent. Txhua tus thiab txhua qhov sib xyaw hauv cov qauv sib xyaw ua ke sib txawv nrog cov khoom siv adsorbent, uas ua rau cov nqi sib txawv rau cov khoom sib txawv. Qhov no tuaj yeem ua rau muaj kev sib cais ntawm cov neeg suav nrog thaum lawv ntws tawm ntawm HPLC kem.
HPLC tau siv rau ntau yam kev siv xws li kev tshuaj xyuas cov qib vitamin D hauv cov ntshav, kev siv yeeb tshuaj tsis raug cai ntawm cov neeg ncaws pob los ntawm kev kuaj xyuas cov tshuaj seem hauv lawv cov zis, txheeb xyuas cov ntsiab lus ntawm cov qauv lom neeg lom zem rau kev tshawb fawb thiab tshuaj xyuas. thiab kev tsim tshuaj.
LCMS yog dab tsi?
Ua kua chromatography-mass spectrometry (LCMS) yog ib qho kev tshuaj ntsuam xyuas uas sib xyaw ua ke lub cev muaj peev xwm sib cais ntawm cov kua chromatography nrog rau kev soj ntsuam huab hwm coj muaj peev xwm ntawm huab hwm coj spectrometry (MS). Cov kua chromatography yog cov txheej txheem sib cais, thiab huab hwm coj spectrometry yog siv los txheeb xyuas qhov loj-rau-them piv ntawm cov khoom them. Kev sib cais ntawm lub cev feem ntau ua tiav los ntawm HPLC thiab lwm yam, LCMS kuj hu ua HPLC-MS. LCMS yog cov txheej txheem tshuaj ntsuam xyuas uas muaj qhov raug siab heev, rhiab heev, thiab qhov tshwj xeeb piv rau HPLC. Yog li, nws muaj txiaj ntsig zoo hauv ntau daim ntawv thov xws li kev tshawb fawb lub hom phiaj, tshuaj xyuas tshuaj, tshuaj xyuas zaub mov, thiab lwm yam. LCMS feem ntau yog siv los cais, txheeb xyuas, txheeb xyuas thiab txheeb xyuas cov khoom siv biochemical ntawm ib qho piv txwv nyob rau hauv muaj cov tshuaj sib tov sib xyaw.
Qhov txawv ntawm HPLC thiab LCMC yog dab tsi?
Acronym thiab txhais ntawm HPLC thiab LCMC
HPLC: HPLC sawv cev rau Kev Ua Haujlwm Siab Ua Kua Dej Chromatography. Nws yog cov txheej txheem kev sib cais uas feem ntau yog siv los cais cov khoom sib cais, txheeb xyuas thiab ntsuas txhua qhov sib xyaw ua ke.
LCMS: LCMS sawv rau Liquid Chromatography thiab Mass Spectrometry. Nws yog cov txheej txheem kev tshuaj ntsuam xyuas uas sib xyaw ua ke lub peev xwm sib cais ntawm lub cev ntawm cov kua chromatography nrog cov peev txheej tsom xam ntawm huab hwm coj spectrometry (MS).
yam ntxwv ntawm HPLC thiab LCMC
Kev faib tawm
HPLC: Qhov no yog Liquid Chromatography txoj kev nkaus xwb.
LCMS: Qhov no yog ua ke ntawm Liquid Chromatography method thiab Mass Spectrometry method.
Efficiency
HPLC: Muab piv rau LCMS, HPLC kev tshuaj xyuas tsawg dua thiab qeeb dua.
LCMS: Piv rau HPLC, kev tshuaj xyuas LCMS yog qhov ua tau zoo thiab nrawm dua.
xov xwm
HPLC: Piv rau LCMS, HPLC kev tshuaj xyuas tsawg dua.
LCMS: Piv rau HPLC, qhov kev tshuaj ntsuam LCMS yog qhov rhiab dua.
Specificity
HPLC: Piv rau LCMS, HPLC kev tshuaj xyuas tsawg dua.
LCMS: Piv rau HPLC, qhov kev tshuaj ntsuam LCMS yog qhov tshwj xeeb.
Kev tseeb
HPLC: HPLC muab qhov tseeb tsawg dua li LCMS rau kev txiav txim siab ntawm qee yam tshuaj.
LCMS: LCMS muab cov txiaj ntsig tseeb dua li HPLC rau kev txiav txim siab ntawm qee yam tshuaj.
Component
HPLC: HPLC tuaj yeem suav tias yog ib feem ntawm LCMS.
LCMS: LCMS tsis tuaj yeem suav tias yog ib feem ntawm HPLC.
Ion Source
HPLC: Ion qhov chaw tsis muaj nyob hauv HPLC ntsuas.
LCMS: Ion qhov chaw muaj nyob hauv LCMS ntsuas.
Applications
HPLC: Ions, polymers, organic molecules, thiab biomolecules tuaj yeem txheeb xyuas siv HPLC.
LCMS: Organic molecules thiab biomolecules tuaj yeem txheeb xyuas. Kev sib piv rau HPLC, LCMS tuaj yeem siv los tshuaj xyuas cov khoom sib tov tsis tiav.
Kev ua haujlwm
HPLC: Daim duab ntawm HPLC ntsuas tau muab rau hauv daim duab 1, thiab nws feem ntau suav nrog autosampler, twj, thiab lub tshuab ntes. Tus qauv qhia txog cov qauv sib xyaw rau hauv lub sijhawm txawb (lub zog sib xyaw ntawm cov kuab tshuaj xws li dej, acetonitrile thiab / lossis methanol) uas hloov mus rau sab. Cov twj tso kua mis xa cov kev xav tau ntws thiab muaj pes tsawg leeg ntawm cov theem mobile los ntawm kab. Cov kem yog ntim nrog cov adsorbent, uas yog ib qho khoom zoo li silica lossis polymers. Lub detector tsim ib lub teeb liab proportionate rau tus nqi ntawm cov qauv constituent muaj nyob rau hauv kem, yog li tso cai rau quantifiable tsom xam ntawm cov piv txwv constituents. Cov cuab yeej HPLC yog tswj hwm, thiab kev txheeb xyuas cov ntaub ntawv yog muab los ntawm digital microprocessor thiab cov neeg siv software.
Daim duab 1: Daim duab ntawm HPLC instrument
LCMS: Daim duab ntawm LCMS ntsuas tau muab rau hauv daim duab 2. Cov qauv rho tawm tau muab tso rau hauv kem uas muaj HPLC. Cov kab no khaws cov qauv metabolites raws li cov cim ntawm lub cev, thiab cov metabolites sib txawv ntws mus rau qhov loj spectrometer ntawm lub sijhawm sib txawv. Mass spectroscopy yog siv rau kev ntsuam xyuas qhov loj ntawm cov khoom, rau kev txiav txim siab cov txheej txheem ntawm cov molecule, thiab kom paub meej cov qauv molecule. Txawm li cas los xij, cov qauv yuav tsum tau ionized los tsim cov molecules them nyiaj txhawm rau txiav txim siab lawv qhov sib piv loj-rau-them. Yog li ntawd, es tsis txhob HPLC cov cuab yeej, LCMS muaj peb lub modules ntxiv xws li hlau qhov chaw, ib tug loj analyzer, thiab ib tug detector. Ib qhov chaw ion tuaj yeem hloov cov qauv roj mus rau hauv ions thiab ib qho kev soj ntsuam loj uas ua rau cov ions los ntawm lawv cov khoom siv electromagnetic teb. Thaum kawg, tus ntsuas ntsuas ntsuas qhov tseem ceeb thiab xa cov ntaub ntawv ntawm txhua ion tam sim no hauv cov qauv. Cov txheej txheem LCMS tuaj yeem siv rau ob qho tib si zoo thiab ntau daim ntawv thov.
Daim duab 2: Daim duab ntawm LCMS instrument
Nyob rau hauv xaus, HPLC yog ib tug kua chromatography txoj kev whereas LCMS yog ib tug ua ke ntawm cov kua chromatography thiab loj spectrometry. Ob txoj kev tshuaj ntsuam xyuas no muaj cov yam ntxwv sib txawv, tab sis lawv tuaj yeem siv los txheeb xyuas thiab txheeb xyuas cov khoom noj muaj pes tsawg leeg, tshuaj, thiab lwm yam bioactive molecules.
