Monoclonal Antibodies vs Polyclonal Antibodies
Cov tshuaj tiv thaiv tiv thaiv kab mob. Qhov tseeb, lub cev muaj cov kab mob tshwj xeeb, uas ua rau cov tshuaj tiv thaiv tshwj xeeb. Lub cev tiv thaiv kab mob yuav txheeb xyuas lub cev txawv teb chaws. Tom qab ntawd nws yuav txiav txim siab seb lub cev txawv teb chaws puas yuav ua mob rau tus kheej cov ntaub so ntswg. Yog tias lub cev tiv thaiv kab mob txheeb xyuas cov ntaub so ntswg ua teeb meem, nws yuav tsim cov tshuaj tiv thaiv kab mob. Antibody ntau lawm yog ib feem ntawm kev tiv thaiv kab mob. B lymphocytes yuav qhib kom tsim cov tshuaj tiv thaiv. Cov B hlwb tuaj yeem txheeb xyuas lub cev txawv teb chaws thaum cov antigen nthuav tawm ntawm tes tsim cov khoom ntawm lub cev txawv teb chaws mus rau B cell. Nyob ntawm qhov kev nthuav qhia cov tshuaj tiv thaiv yuav tawm tsam qhov tshwj xeeb ntawm lub cev txawv teb chaws. Hauv lwm lo lus, B cell tsim cov tshuaj tiv thaiv kab mob rau cov tshuaj tiv thaiv kab mob rau ntau yam ntawm cov tshuaj tiv thaiv.
Cov B hlwb yuav faib thiab tsim cov hlwb zoo sib xws. Cov cell no yuav tsim cov tshuaj tiv thaiv tib yam. B cell uas yog qhib rau lwm qhov sib txawv ntawm cov antigen kuj faib thiab tsim cov hlwb tshiab. Cov kab ntawm tes uas yog tsim los ntawm B cell hu ua CLONE. Cov antigen tuaj yeem qhib ntau lub hlwb B thiab cov hlwb no faib thiab tsim ntau lub clones. Qhov no yuav raug hu ua poly clone ntawm B hlwb.
Ntau xyoo dhau los, cov tshuaj tiv thaiv poly clonal yog tsim los rau kev kho mob los ntawm kev txhaj tshuaj txawv teb chaws rau tsiaj / noog (nees, npua, qaib) thiab sau cov tshuaj tiv thaiv los ntawm lawv cov ntshav (ntshav). Cov tshuaj tiv thaiv yog purified thiab siv nyob rau hauv tib neeg. Txawm li cas los xij raws li cov tshuaj tiv thaiv no kuj txawv teb chaws rau tib neeg, nws ua rau muaj kev tsis haum tshuaj thiab tsim cov tshuaj tiv thaiv kab mob tiv thaiv kab mob.
Kev nce qib ntawm noob caj noob ces muab kev tsim kho caj ces. Cov thev naus laus zis thev naus laus zis tau pab tus kws tshawb fawb los ntxig cov DNA rau hauv cov kab mob plasmids thiab tsim cov tshuaj tiv thaiv. Niaj hnub no cov tshuaj tiv thaiv yog tsim los ntawm recombinant technology.
Mono clonal antibodies yog tsim los ntawm tsuas yog ib kab ntawm tes (clone). Cov tshuaj tiv thaiv zoo tshaj plaws uas tsim cov B cell yuav raug xaiv thiab tsuas yog cov tshuaj tiv thaiv raug sau. Qhov no yuav pab txo cov kev mob tshwm sim ntawm cov tshuaj tiv thaiv kab mob thiab ua kom cov tshuaj tiv thaiv kab mob.
Nyob rau hauv cov nqe lus kho mob cov tshuaj tiv thaiv kab mob coj mus muag tau sau npe ua IMMUNOGLOBULIN.
In summary, • Tib neeg lub cev tuaj yeem tsim cov tshuaj tiv thaiv kab mob los tiv thaiv lub cev ntawm lub cev txawv teb chaws.
• Cov tshuaj tiv thaiv tuaj yeem npaj ua lag luam thiab cov tshuaj tiv thaiv no hu ua Immunoglobulins.
• Cov tshuaj tiv thaiv polyclonal yog tsim los ntawm ntau yam clones ntawm B lymphocytes.
• Monoclonal antibodies yog sau los ntawm tsuas yog ib clone ntawm B cell.
• Recombinant thev naus laus zis pab tsim cov tshuaj tiv thaiv.
