YFluorescence vs Luminescence
Luminescence yog txheej txheem ntawm emitting lub teeb. Qhov no tuaj yeem tshwm sim los ntawm ntau txoj kev. Hauv tsab xov xwm no, peb yuav tham txog cov txheej txheem thiab hom txheej txheem teeb pom kev zoo.
Fluorescence yog dab tsi?
Electrons nyob rau hauv ib lub atom los yog ib lub molecule tuaj yeem nqus tau lub zog hauv hluav taws xob hluav taws xob thiab yog li ua rau lub zog sab sauv. Lub zog sab sauv no tsis ruaj khov; yog li ntawd, electron nyiam rov qab los rau hauv av. Thaum rov qab los, nws emits lub absorbed wavelength. Nyob rau hauv cov txheej txheem so, lawv emit ntau zog li photons. Cov txheej txheem so no yog hu ua fluorescence. Fluorescence yuav siv qhov chaw nrawm dua thiab feem ntau ua tiav hauv li 10-5 s lossis tsawg dua lub sijhawm los ntawm lub sijhawm excitation. Nyob rau hauv atomic fluorescence, gaseous atoms fluoresce thaum lawv raug hluav taws xob nrog lub wavelength uas raws nraim ib qho ntawm cov kab nqus ntawm lub caij. Piv txwv li, gaseous sodium atoms nqus thiab excite los ntawm absorbing 589 nm hluav taws xob. Kev so yuav siv sij hawm qhov chaw tom qab qhov no los ntawm remission ntawm fluorescent hluav taws xob ntawm qhov zoo tib yam wavelength. Vim li no, peb tuaj yeem siv fluorescence los txheeb xyuas cov ntsiab lus sib txawv. Thaum excitation thiab remission wavelengths zoo ib yam, qhov tshwm sim emission hu ua resonance fluorescence. Lwm yam tshaj li fluorescence, muaj lwm cov txheej txheem uas ib qho kev zoo siab atom lossis molecule tuaj yeem tso nws lub zog ntau dhau thiab so rau nws lub xeev hauv av. Nonradiative so thiab fluorescence emissions yog ob qho tseem ceeb xws li cov txheej txheem. Vim muaj ntau yam mechanisms, lub neej ntawm lub xeev zoo siab yog luv luv. Cov txheeb ze ntawm cov molecules uas fluoresce yog me me vim tias fluorescence yuav tsum muaj cov yam ntxwv uas ua rau qeeb tus nqi ntawm qhov tsis muaj hluav taws xob so thiab txhim kho tus nqi fluorescence. Hauv feem ntau cov molecules, cov yam ntxwv no tsis nyob ntawd; Yog li ntawd, lawv tau txais kev so tsis muaj hluav taws xob, thiab fluorescence tsis tshwm sim. Molecular fluorescence bands yog tsim los ntawm ntau cov kab sib txuas; yog li ntawd, feem ntau nws nyuaj rau daws.
Luminescence yog dab tsi?
Luminescence yog txheej txheem ntawm emitting lub teeb los ntawm ib yam khoom. Qhov emission no tsis yog vim cua sov; yog li ntawd, nws yog ib daim ntawv ntawm lub cev txias hluav taws xob. Muaj ob peb hom luminescence li bioluminescence, chemiluminescence, electrochemiluminescence, electroluminescence, photoluminescence, thiab lwm yam. Bioluminescence yog lub emission ntawm lub teeb los ntawm cov kab mob nyob. Piv txwv li, fireflies tuaj yeem xav txog. Qhov no yog txheej txheem ntuj tsim. Lub teeb raug tso tawm raws li qhov tshwm sim ntawm cov tshuaj tiv thaiv uas tshwm sim hauv lub cev. Hauv fireflies, thaum cov tshuaj hu ua luciferin reacts nrog oxygen, lub teeb yog tsim. Cov tshuaj tiv thaiv no yog catalyzed los ntawm enzyme luciferase. Chemiluminescence yog tshwm sim los ntawm cov tshuaj tiv thaiv tshuaj. Qhov tseeb, bioluminescence yog hom chemiluminescence. Piv txwv li, cov tshuaj tiv thaiv catalyzed ntawm luminal thiab hydrogen peroxide tsim lub teeb. Electrochemiluminescence yog ib hom luminescence tsim thaum lub sij hawm cov tshuaj tiv thaiv electrochemical.
Qhov txawv ntawm Fluorescence thiab Luminescence yog dab tsi?
• Fluorescence yog hom luminescence.
• Fluorescence yog tshwm sim los ntawm kev nqus ntawm photons, yog li nws yog hom photoluminescence.
• Los ntawm cov yam ntxwv atomic fluorescence, cov ntsiab lus tuaj yeem txheeb xyuas.
• Fluorescence tshwm sim hauv atoms lossis molecules, whereas luminescence tuaj yeem tshwm sim hauv cov kab mob, kev daws teeb meem, molecules, thiab lwm yam.
