Qhov sib txawv tseem ceeb ntawm alpha beta thiab gamma amylase yog tias alpha amylase enzyme ua haujlwm los ntawm kev ua haujlwm ntawm qhov chaw tsis sib xws nrog cov hmoov txhuv nplej siab thiab beta amylase ua haujlwm los ntawm qhov tsis txo qhov kawg ntawm polysaccharide los ntawm cleaving alpha-1, 4 glycosidic. bonds, whereas gamma amylase ua haujlwm los ntawm qhov tsis txo qhov kawg ntawm cov polysaccharide los ntawm cleaving ob leeg alpha-1, 4 glycosidic bonds thiab alpha-1, 6 glycosidic bonds.
Amylase yog ib qho enzyme uas tuaj yeem ua rau lub hydrolysis ntawm starch rau hauv cov suab thaj molecules. Cov enzyme no muaj nyob rau hauv cov qaub ncaug ntawm tib neeg thiab lwm yam tsiaj.
Alpha Amylase yog dab tsi?
Alpha amylase yog ib qho enzyme uas tuaj yeem hydrolyze alpha bonds ntawm polysaccharides loj. Cov no yog alpha-txuas polysaccharides, nrog rau cov hmoov txhuv nplej siab thiab glycogen. Cov tshuaj tiv thaiv hydrolysis no ua rau luv luv chains ntawm dextrin thiab m altose. Qhov no yog ib hom amylase loj uas peb tuaj yeem pom hauv tib neeg thiab lwm yam tsiaj. Ntxiv mus, peb tuaj yeem pom nws hauv cov noob uas muaj cov hmoov txhuv nplej siab, thiab nws tseem zais los ntawm ntau hom fungi. Cov enzyme no yog ib tug tswv cuab ntawm tsev neeg glycoside hydrolase 13.
Daim duab 01: Alpha Amylase Enzyme
Industrially, cov enzyme no muaj txiaj ntsig zoo hauv kev tsim ethanol. Nws tuaj yeem rhuav cov hmoov txhuv nplej siab hauv cov nplej rau hauv cov suab thaj fermentable. Kev kho cov pob kws nrog cov alpha amylase enzyme yog thawj kauj ruam hauv kev tsim cov pob kws muaj fructose phoov. Cov txheej txheem no ua rau luv luv chains ntawm oligosaccharide qab zib. Tsis tas li ntawd, ib qho alpha amylase enzyme hu ua termamyl muaj txiaj ntsig zoo hauv qee cov tshuaj ntxuav tais diav thaum ntxuav tais diav thiab siv cov hmoov txhuv nplej siab tshem tawm.
Ntxiv mus, muaj ntau txoj hauv kev uas peb tuaj yeem siv los txiav txim siab txog kev ua haujlwm ntawm alpha amylase enzyme. Txoj kev tshaj plaws yog kev kuaj cov hmoov txhuv nplej siab-iodine. Nws yog kev txhim kho ntawm kev kuaj iodine. Qhov kev sim no nyob ntawm kev hloov xim ntawm cov hmoov txhuv nplej siab-iodine complex. Qhov kev ntsuam xyuas tseem ceeb tshaj plaws yog Phadebas amylase test.
Beta Amylase yog dab tsi?
Beta amylase yog ib qho enzyme uas tuaj yeem ua rau lub hydrolysis ntawm alpha-D-glucosidic linkages hauv polysaccharides, tshem tawm m altose units los ntawm polysaccharide. Nws yog ib qho enzyme uas muaj cov tshuaj npe 4-alpha-D-glucan m altohydrolase.
Daim duab 02: Beta Amylase
Cov substrates loj ntawm beta amylase yog cov hmoov txhuv nplej siab, glycogen, thiab polysaccharides, uas tsim cov beta-m altose los ntawm kev hloov pauv. Ntxiv mus, peb tuaj yeem pom cov enzyme no hauv cov kab mob, fungi, thiab nroj tsuag. Ntawm lawv, cov kab mob thiab cov khoom noj khoom haus yog cov ntaub ntawv uas muaj cua sov tshaj plaws. Lub beta amylase enzyme ua los ntawm qhov tsis txo qhov kawg ntawm cov saw hlau polysaccharide. Pib los ntawm qhov kawg, qhov enzyme no catalyzes hydrolysis ntawm thib ob alpha-1, 4 glycosidic daim ntawv cog lus los ntawm kev tshem tawm ob lub qabzib, tshwj xeeb tshaj yog m altose ib zaug.
Thaum xav txog cov peev txheej ntawm beta amylase, nws tshwm sim hauv daim ntawv tsis muaj zog hauv cov noob germination ua ntej. Feem ntau cov microbes tsim cov enzyme no los ntawm degrading extracellular starches. Tsis muaj beta amylase nyob rau hauv cov ntaub so ntswg tsiaj, txawm hais tias nws tuaj yeem tshwm sim hauv cov kab mob uas muaj nyob hauv lub plab zom mov. Rau qhov enzyme no ua haujlwm zoo, pH 4.0 - 5.0 yog qhov zoo tagnrho.
Gamma Amylase yog dab tsi?
Gamma amylase yog ib hom amylase uas tuaj yeem tshem tawm alpha 1, 6-glycosidic linkages thiab alpha 1, 4-glycosidic bonds hauv amylose, amylopectin. Qhov cleavage no tshwm sim ntawm qhov tsis txo qhov kawg. Cov khoom ntawm no cleavage yog qabzib. Ntawm tag nrho lwm cov qauv ntawm amylase enzyme, gamma amylase muaj cov acidic zoo tshaj plaws ph ntau. Nws muaj qhov ua haujlwm zoo pH ntawm thaj tsam 3.0. Cov gamma amylase enzymes no tuaj yeem yog cov tsev neeg glycosidic hydrolase sib txawv, suav nrog glycosidic hydrolase 15 tsev neeg hauv hom fungi, glycosidic hydrolase tsev neeg 31 hauv tib neeg, thiab glycosidic hydrolase 97 hauv cov kab mob.
Qhov txawv ntawm Alpha Beta thiab Gamma Amylase yog dab tsi?
Amylase yog ib qho enzyme uas tuaj yeem ua rau lub hydrolysis ntawm starch rau hauv qab zib molecules. Cov enzyme no muaj nyob hauv cov qaub ncaug ntawm tib neeg thiab qee cov tsiaj txhu. Qhov sib txawv tseem ceeb ntawm alpha beta thiab gamma amylase yog tias alpha amylase enzyme ua haujlwm los ntawm kev ua haujlwm ntawm qhov chaw random raws cov hmoov txhuv nplej siab thiab beta amylase ua haujlwm los ntawm qhov tsis txo qhov kawg ntawm polysaccharide los ntawm cleaving alpha-1, 4 glycosidic bonds. Txawm li cas los xij, gamma amylase ua haujlwm los ntawm qhov tsis txo qhov kawg ntawm polysaccharide los ntawm cleaving alpha-1, 4 glycosidic bonds thiab alpha-1, 6 glycosidic bonds.
Cov hauv qab no infographic teev qhov sib txawv ntawm alpha beta thiab gamma amylase hauv daim ntawv tabular rau ib sab sib piv.
Summary – Alpha vs Beta vs Gamma Amylase
Amylase yog ib qho enzyme uas tuaj yeem ua rau lub hydrolysis ntawm starch rau hauv cov suab thaj molecules. Qhov sib txawv tseem ceeb ntawm alpha beta thiab gamma amylase yog tias alpha amylase enzyme ua haujlwm los ntawm kev ua haujlwm ntawm qhov chaw random raws cov hmoov txhuv nplej siab thiab beta amylase ua haujlwm los ntawm qhov tsis txo qhov kawg ntawm polysaccharide los ntawm cleaving alpha-1, 4 glycosidic bonds, whereas gamma amylase. ua haujlwm los ntawm qhov tsis txo qhov kawg ntawm cov polysaccharide los ntawm cleaving ob leeg alpha-1, 4 glycosidic bonds thiab alpha-1, 6 glycosidic bonds.
