Qhov sib txawv tseem ceeb ntawm isoschizomers thiab neoschizomers yog tias isoschizomers yog txwv enzymes uas muaj tib qhov kev lees paub thiab txiav cov DNA ntawm tib txoj haujlwm, thaum neoschizomers yog txwv enzymes uas muaj tib qhov kev lees paub tab sis cleave DNA ntawm ntau txoj haujlwm..
Kev txwv enzymes lossis txwv endonucleases yog cov enzymes uas txiav DNA rau hauv cov khoom tawg ntawm lossis ze rau qhov chaw paub tshwj xeeb. Lawv txiav DNA ntawm qhov chaw txwv. Kev txwv enzymes feem ntau yog muab faib ua tsib pawg raws li cov qauv, txawm tias lawv txiav lawv cov DNA substrate ntawm lawv qhov chaw lees paub, thiab seb qhov chaw lees paub thiab qhov chaw tawg yog cais los ntawm ib leeg. Ntau tshaj 3600 qhov kev txwv endonucleases tau raug txheeb xyuas. Kwv yees li 800 qhov txwv enzymes muaj nyob rau hauv kev lag luam. Isoschizomers thiab neoschizomers yog ob hom kev txwv enzymes raws li qhov chaw lees paub thiab qhov tshwj xeeb cleavage.
Isoschizomers yog dab tsi?
Isoschizomers yog txwv enzymes uas muaj tib qhov kev lees paub thiab tshem tawm DNA ntawm tib txoj haujlwm. Cov kev txwv enzymes no muaj tib yam tshwj xeeb. Thawj qhov kev txwv enzymes uas paub txog qhov muab ib ntus yog lub npe hu ua tus qauv, thaum tag nrho cov tom qab tau txheeb xyuas cov kev txwv enzymes uas paub txog tib lub sijhawm yog hu ua isoschizomers. Txawm li cas los xij, isoschizomers yuav txawv ntawm qhov chaw nyiam, cov xwm txheej tshwm sim, methylation rhiab heev, thiab hnub qub ua haujlwm. Isoschizomers yog cais los ntawm ntau hom kab mob. Yog li ntawd, lawv yuav xav tau cov tshuaj tiv thaiv sib txawv. Hauv qee qhov xwm txheej, tsuas yog ib qho ntawm ib khub ntawm isoschizomers tuaj yeem lees paub ob qho tib si methylated thiab unmethylated cov ntaub ntawv txwv qhov chaw. Ntawm qhov tod tes, lwm qhov kev txwv enzyme tuaj yeem lees paub tsuas yog daim ntawv tsis muaj tshuaj lom ntawm qhov chaw txwv. Qhov tshwj xeeb ntawm isoschizomers no pab txheeb xyuas lub xeev methylation ntawm qhov chaw txwv thaum cais tawm ntawm lub xeev cov kab mob.
Piv txwv li, AgeI thiab BshT1 lees paub thiab cleave 5'-A↓CCGGT hauv tib tus qauv. HpaII thiab MSPI yog lwm khub ntawm isoschizomers. Lawv ob leeg paub txog ntu 5'-C↓CGG-3' thaum nws tsis muaj methylated. Tab sis thaum thib ob C ntawm qhov sib lawv liag yog methylated, tsuas yog MSPI tuaj yeem paub qhov sib lawv liag thaum HpaII tsis tuaj yeem paub nws.
Neochizomers yog dab tsi?
Neoschizomers yog txwv cov enzymes uas muaj tib qhov kev lees paub tab sis cais DNA ntawm ntau txoj haujlwm. Hauv qee qhov tshwj xeeb kev siv tshuaj lom neeg molecular, qhov no yog qhov muaj txiaj ntsig zoo heev. Cov neoschizomers yog ib feem ntawm isoschizomers. Cov piv txwv zoo rau cov neoschizomers yog SmaI (5'-CCC↓GGG-3') thiab XmaI (5'-C↓CCGGG-3'); ob leeg paub txog 5'-CCCGGG-3' ib ntus tab sis cleave lawv ntawm lwm txoj haujlwm. Yog li, ob qho kev txwv enzymes no tsim ntau hom kev xaus. Nyob rau hauv rooj plaub no, SmaI tsim blunt kawg, thiab XmaI tsim 5 'protruding xaus.
Daim duab 01: Neoschizomers
Lwm qhov piv txwv yog MaeII thiab Tail txwv enzymes khub. Prototype MaeII (A↓CGT) tsim cov khoom DNA nrog 2-base 5'extension, thiab neoschizomer Tail (ACGT↓) tsim DNA tawg nrog 4-base3' extension.
Dab tsi zoo sib xws ntawm Isoschizomers thiab Neoschizomers?
- Isoschizomers thiab neoschizomers yog ob hom txwv enzymes.
- Lawv muaj feem ntau pom hauv prokaryotes.
- Lawv yog ib feem ntawm kev txwv-hloov kho (RM) system pom nyob rau hauv cov kab mob thiab archaea.
- ob leeg yog protein molecules.
- Ob leeg txiav DNA ua ntu zus.
Qhov txawv ntawm Isoschizomers thiab Neoschizomers yog dab tsi?
Isoschizomers yog txwv enzymes uas muaj tib qhov kev lees paub thiab tshem tawm cov DNA ntawm tib txoj haujlwm, thaum neoschizomers yog txwv enzymes uas muaj tib qhov kev lees paub tab sis cleave DNA ntawm ntau txoj haujlwm. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm isoschizomers thiab neoschizomers. Tsis tas li ntawd, isoschizomers muaj tib yam tshwj xeeb thaum neoschizomers muaj qhov tshwj xeeb sib txawv.
Cov infographic hauv qab no teev qhov sib txawv ntawm isoschizomers thiab neoschizomers hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – Isoschizomers vs Neoschizomers
Kev txwv enzymes yog DNA txiav enzymes. Lawv kuj hu ua molecular txiab. Muaj ntau hom kev txwv enzymes. Raws li qhov chaw lees paub thiab qhov tshwj xeeb cleavage, txwv enzymes yog ob hom isoschizomers thiab neoschizomers. Isoschizomers muaj tib qhov kev lees paub thiab cais cov DNA ntawm tib txoj haujlwm thaum neoschizomers muaj tib qhov kev lees paub tab sis cleave DNA ntawm ntau txoj haujlwm. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm isoschizomers thiab neoschizomers.
