Qhov sib txawv tseem ceeb ntawm isoschizomers thiab isocaudomers yog tias isoschizomers lees paub thiab tshem tawm tib qhov kev txwv lossis kev lees paub qhov chaw thaum isocaudomers paub txog qhov sib txawv me ntsis thiab cleave ntawm tib qhov chaw.
Kev txwv enzymes lossis txwv tsis pub endonucleases yog hom nucleases uas tshem tawm ob txoj hlua DNA. Lawv muaj peev xwm paub txog cov kab ke tshwj xeeb hu ua qhov chaw txwv, uas yog palindromic hauv keeb kwm. Cov kab mob tsim cov enzymes txwv cov kab mob txawv teb chaws, tshwj xeeb tshaj yog tawm tsam cov kab mob. Nws yog ib hom kev tiv thaiv mechanism uas qhia los ntawm cov kab mob. Nyob ntawm cov qauv ntawm cov enzymes thiab qhov xwm txheej ntawm kev txiav, muaj peb hom kev txwv enzymes xws li hom I txwv enzymes, hom II txwv enzymes, thiab hom II txwv enzymes. Ntxiv mus, nyob ntawm qhov kev ua haujlwm biochemical thiab qhov chaw ntawm kev sib cais, muaj peb hom kev txwv enzymes xws li isoschizomers, neoschizomers thiab isocaudomers.
Isoschizomers yog dab tsi?
Isoschizomers yog ib hom kev txwv enzyme uas los ntawm ntau qhov chaw tab sis lees paub thiab cais tib qhov chaw txwv hauv DNA. Yog li ntawd, ob isoschizomers muaj tib qhov chaw lees paub. Lawv cleave ntawm tib lub xaib, yog li lawv tshwj xeeb rau tib qhov kev lees paub ib ntus. Piv txwv li, txwv enzymes SphI thiab BbuI yog ob isoschizomers uas paub thiab cleave ntawm CGTAC/G.
Daim duab 01: Kev txwv Enzyme
Lwm khub isoschizomers yog HpaII thiab MspI. Lawv ob leeg paub qhov sib lawv liag 5′-CCGG-3′ thiab tshem tawm cov DNA. Ntxiv mus, Bsp EI thiab Acc III kuj yog isoschizomers uas yog los ntawm Bacillus hom thiab Acinetobacter calcoaceticus, raws li. Kev rho tawm isoschizomers tuaj yeem ua los ntawm cov kab mob sib txawv. Txij li thaum lawv los ntawm cov kab mob sib txawv, lawv cov kev txwv yuav txawv ntawm ib leeg.
Isocaudomers yog dab tsi?
Isocaudomers yog ib hom kev txwv enzyme uas paub txog qhov sib txawv me ntsis, tab sis lawv cleave ntawm tib qhov chaw los tsim tib qhov kawg. Isoaudomers kuj tuaj ntawm cov kab mob sib txawv. Sau 3a thiab BamHI yog ib khub ntawm isocaudomers. Lawv tsim 5'-GATC-3' nplaum kawg tom qab cleavage ntawm tib qhov chaw. Lwm khub ntawm isocaudomers yog NcoI los ntawm Nocardia corallina thiab PagI los ntawm Pseudomonas alcaligenes. Ob lub enzymes no tseem khi ntawm qhov sib txawv ntawm qhov sib txawv tab sis txiav ntawm tib qhov chaw thiab tsim tib lub nplaum kawg. Yog li ntawd, kev tsim cov txiav xaus yog sib xws, thiab lawv tuaj yeem txuas rau ib leeg. XhoI, PspXI thiab SalI kuj yog isocaudomers.
Dab tsi zoo sib xws ntawm Isoschizomers thiab Isocaudomers?
- Isoschizomers thiab isocaudomers yog ob hom txwv enzymes.
- Ob tug isoschizomers muaj tib qhov chaw digestion, zoo ib yam li ob isocaudomers uas muaj tib qhov chaw digestion.
- Lawv yog prokaryotic hauv keeb kwm.
- Ob leeg muaj qhov txwv tsis pub lees paub nrog rau qhov chaw zom zaub mov.
- Lawv tsim cov xaus uas sib haum xeeb.
- Ntxiv rau, cov enzymes no yog cov cuab yeej siv tau zoo hauv cov tshuab DNA recombinant.
Qhov txawv ntawm Isoschizomers thiab Isocaudomers yog dab tsi?
Isoschizomers yog txwv enzymes uas paub txog tib DNA ib ntus thiab cleave ntawm tib qhov chaw txwv, thaum isocaudomers yog txwv enzymes uas paub txog cov DNA sib txawv me ntsis tab sis tsim tib lub nplaum kawg los ntawm kev txiav ntawm tib qhov chaw. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm isoschizomers thiab isocaudomers.
Cov hauv qab no infographic tabulates qhov sib txawv ntawm isoschizomers thiab isocaudomers.
Summary – Isoschizomers vs Isocaudomers
Isoschizomers yog txwv enzymes uas paub txog tib lub DNA sib lawv liag thiab sib cais ntawm tib qhov chaw. Ntawm qhov tod tes, isocaudomers yog txwv cov enzymes uas paub txog qhov sib txawv me ntsis thiab cleave ntawm tib qhov chaw ua rau tib qhov kawg. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm isoschizomers thiab isocaudomers. SphI, BbuI, HpaII thiab MspI yog ob peb yam piv txwv ntawm isoschizomers thaum Sau3a thiab BamHI yog ob qho piv txwv ntawm isocaudomers. Ob leeg isoschizomers thiab isocaudomers yog ib txwm tsim los ntawm cov kab mob.
