Qhov sib txawv ntawm qhov tseem ceeb ntawm qhov nruab nrab ntawm qhov nruab nrab thiab qhov chaw ntawm cov proteins yog qhov tseem ceeb thiab cov proteins nyob rau hauv cov ntaub so ntswg mus tas li nyob rau hauv cov plasma membrane thaum peripheral protein yog ib ntus cuam tshuam nrog plasma membrane.
Cov plasma membrane muaj cov molecules uas tsis yog phospholipids. Cov molecules no tuaj yeem yog cov protein lossis carbohydrates. Lub plasma membrane muaj qee hom proteins uas ua lub luag haujlwm tseem ceeb hauv kev ua haujlwm ntawm tes. Ib daim nyias nyias yog ib qho molecule uas txuas rau los yog koom nrog cov cell membrane ntawm ib lub cell los yog ib qho organelle. Lawv tuaj yeem muab faib ua ntau hom raws li lawv txoj kev koom tes nrog cov membrane. Integral peripheral thiab deg proteins yog membrane proteins uas tseem ceeb heev rau kev ua ntawm tes.
Cov Proteins Integral yog dab tsi?
Integral protein yog ib daim nyias nyias uas kaw nws tus kheej mus rau hauv lub cell membrane mus tas li. Yog li ntawd, lawv yog cov khoom ruaj khov ntawm cov kab mob lom. Integral protein feem ntau muab faib ua ob hom xws li transmembrane proteins thiab ib qho monotopic proteins. Transmembrane membrane protein spans tag nrho cov plasma membrane. Ntawm qhov tod tes, ib qho monotopic protein txuas mus tas li rau daim nyias nyias los ntawm ib sab. Cov qauv peb-dimensional ntawm kwv yees li 160 qhov sib txawv ntawm cov proteins tau raug txiav txim siab ntawm atomic daws los ntawm nuclear magnetic resonance txog tam sim no.
Daim duab 01: Plasma Membrane
Kawm cov proteins no nyuaj heev vim muaj teeb meem hauv lawv cov extraction thiab crystallization. Tsis tas li ntawd, cov qauv ntawm ntau yam ntawm cov protein no tau nkag mus rau hauv cov ntaub ntawv cov ntaub ntawv protein (PDB). Cov txheej txheem membrane protein ua haujlwm ntau yam sib txawv. Nws tuaj yeem ua haujlwm raws li kev thauj mus los, kev sib txuas, cov channel, receptors, enzymes, cov txheej txheem ntawm daim nyias nyias, thiab cell adhesion proteins. Tsis tas li ntawd, nws kuj muaj nyob rau hauv tsub zuj zuj thiab transduction ntawm lub zog. Qee qhov piv txwv yog cell adhesion proteins, rhodopsin thiab qabzib permease, thiab lwm yam.
Peripheral Proteins yog dab tsi?
Peripheral protein yog ib daim nyias nyias uas ua ke nrog cov plasma membrane ib ntus. Nws feem ntau txuas rau cov txheej txheem membrane cov protein kom nkag mus rau thaj tsam peripheral ntawm lipid bilayer. Piv txwv li, kev tswj hwm cov protein subunits ntawm ion channels thiab transmembrane receptors tej zaum yuav raug txhais tias yog peripheral proteins. Cov proteins no txuas rau cov proteins lossis cov lipid bilayers los ntawm kev sib xyaw ua ke ntawm hydrophobic, electrostatic, thiab lwm yam kev sib cuam tshuam tsis sib xws.
Peripheral protein tuaj yeem sib cais tom qab kev kho mob nrog cov polar reagent (kev daws teeb meem nrog pH siab lossis siab ntsev concentration). Lawv tuaj yeem hloov kho tom qab hloov pauv nrog ntxiv fatty acid, diacylglycerol lossis prenyl chains. Tsis tas li ntawd, lub luag haujlwm tseem ceeb ntawm peripheral protein yog kev txhawb nqa ntawm tes, kev sib txuas lus, enzymes thiab molecular hloov pauv xws li hloov hluav taws xob.
Ntog Proteins yog dab tsi?
Cov proteins saum npoo yog cov txheej txheem membrane uas txuas mus tas li los yog hla cov txheej txheem ntawm tes ntawm cov kab mob ntau dua. Feem ntau cov cell saum npoo av muaj cov khoom noj carbohydrates. Nto protein yog ib qho tseem ceeb rau txoj hauv kev uas lub cell cuam tshuam nrog ib puag ncig nws.
Qee cov proteins saum npoo tau raug rau sab nraud ntawm daim nyias nyias thiab tau txuas cov carbohydrate pawg rau lawv cov chaw sab nrauv. Lawv hu ua glycoproteins. Lawv muaj cov haujlwm sib txawv xws li cov khoom siv, lubricants, cov tshuaj hormones, enzymes, thauj cov molecules, receptors, antifreeze proteins, kev tswj ntawm kev loj hlob, homeostasis, thiab lwm yam. Ntxiv mus, cell nto protein yog ib qho tseem ceeb heev nyob rau hauv biomedical science li 66% ntawm cov neeg pom zoo tshuaj teev. hauv DrugBank database phiaj lub cell nto protein.
Similarities ntawm Integral Peripheral thiab Surface Proteins
- Integral peripheral thiab nto proteins yog membrane proteins.
- lawv yog tsim los ntawm cov amino acids.
- Txhua tus ntawm lawv txuas nrog lipid bilayer ntawm cov hlwb lom.
- Lawv tseem ceeb heev hauv cell ciaj sia.
Qhov Sib Txawv Ntawm Ib Nrab Peripheral thiab Nto Proteins
Cov proteins ib txwm thiab ntog nyob rau hauv cov ntshav plasma mus tas li, thaum cov protein peripheral cuam tshuam nrog cov plasma membrane ib ntus. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm cov khoom siv peripheral thiab cov proteins saum npoo.
Cov infographic hauv qab no qhia ntau qhov sib txawv ntawm cov khoom siv peripheral thiab cov proteins saum npoo hauv daim ntawv.
Summary – Integral vs Peripheral vs Surface Proteins
Membrane proteins sawv cev rau cov chav kawm tseem ceeb ntawm cov proteins koom nrog hauv cov txheej txheem tseem ceeb ntawm tes thiab lub cev. Lub membrane protein yog ib qho molecule txuas nrog los yog txuam nrog cov cell membrane ntawm ib lub cell los yog ib qho organelle. Lawv tuaj yeem muab faib ua ntau hom raws li lawv txoj kev koom tes nrog cov membrane. Integral peripheral thiab nto proteins yog ib qho tseem ceeb heev membrane proteins. Integral thiab nto proteins nyob mus ib txhis embedded nyob rau hauv lub plasma membrane. Nyob rau hauv sib piv, peripheral protein yog ib ntus txuam nrog plasma membrane. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm cov khoom siv peripheral thiab cov proteins saum npoo.
