Qhov sib txawv tseem ceeb ntawm kev ua kom tsis zoo thiab degradation ntawm cov protein yog tias nyob rau hauv denaturation ntawm cov protein, quaternary, tertiary thiab theem nrab cov qauv raug cuam tshuam, tab sis cov qauv tseem ceeb tseem nyob twj ywm thaum, nyob rau hauv degradation ntawm cov protein, thawj cov qauv ntawm cov protein yog. rhuav tshem, tab sis qhov thib ob, tertiary qauv tseem nyob tsis tu ncua.
Denaturation thiab degradation ntawm cov proteins yog cov kauj ruam tseem ceeb hauv kev ua cov proteins hauv cell. Lawv yog cov txheej txheem cellular tseem ceeb heev. Nyob rau hauv lub denaturation ntawm cov protein, cov protein poob nws cov kev ua ub no vim hais tias kev ua haujlwm lom neeg yog ncaj qha nyob ntawm nws cov qauv. Txawm li cas los xij, cov proteins uas degraded tseem tuaj yeem muaj cov qauv theem nrab lossis theem nrab.
Denaturation ntawm Protein yog dab tsi?
Denaturation yog cov txheej txheem uas cov protein poob nws cov qauv quaternary, tertiary qauv, thiab cov qauv theem nrab tam sim no nyob rau hauv lub xeev. Tab sis cov qauv tseem ceeb ntawm cov protein tseem nyob tsis zoo. Nws tuaj yeem ua tiav los ntawm kev siv cov kev ntxhov siab sab nraud, cov tebchaw xws li cov kua qaub uas muaj zog los yog lub hauv paus, cov ntsev tsis muaj zog, cov organic hnyav (cawv, chloroform) thiab hluav taws xob lossis cua sov. Yog hais tias cov proteins nyob rau hauv lub cell tau denatured, nws ua rau muaj kev cuam tshuam ntawm kev ua ntawm tes, tej zaum cell tuag. Hauv cov protein denaturation, cov protein poob nws txoj haujlwm lom neeg. Cov denatured proteins tuaj yeem muaj ntau yam ntawm cov yam ntxwv xws li kev hloov pauv, poob ntawm solubility, thiab kev sib sau ua ke vim raug rau cov pab pawg hydrophobic. Denatured proteins poob lawv cov qauv 3D; yog li ntawd, lawv tsis tuaj yeem ua haujlwm, raws li tau hais ua ntej.
Daim duab 01: Kev hloov pauv ntawm cov protein
Protein folding pab globular lossis membrane proteins ua lawv txoj haujlwm kom raug. Lawv yuav tsum tau muab tais rau hauv txoj cai kom ua tiav txoj haujlwm raug. Txawm li cas los xij, H bonds uas ua lub luag haujlwm tseem ceeb hauv folding yog qhov tsis muaj zog thiab yog li yooj yim cuam tshuam los ntawm tshav kub, acidity, sib txawv ntsev concentration thiab lwm yam kev ntxhov siab uas tuaj yeem cuam tshuam cov protein. Qhov no yog vim li cas homeostasis tseem ceeb heev hauv ntau hom kev ua neej. Cov piv txwv zoo tuaj yeem pom hauv kev ua noj ntau yam khoom noj, xws li cov qe boiled ua tawv thiab cov nqaij siav ua khov.
YYam Degradation ntawm Protein yog dab tsi?
Protein degradation tej zaum yuav tshwm sim nyob rau hauv lub cellular los yog extracellularly. Hauv cov protein degradation, thawj cov qauv ntawm cov protein raug rhuav tshem, tab sis cov qauv theem nrab thiab theem nrab tseem nyob tsis zoo. Nyob rau hauv cov zaub mov digestion, lub digestive enzyme tso tawm rau hauv ib puag ncig rau extracellular digestion. Proteolytic cleavage lov cov proteins rau hauv me me peptides thiab amino acids kom lawv tau nkag tau yooj yim. Nyob rau hauv cov tsiaj, cov zaub mov yuav ua tau extracellularly nyob rau hauv tshwj xeeb lub cev los yog lub plab. Tab sis nyob rau hauv ntau cov kab mob, zaub mov yuav ua tiav los ntawm internalization los ntawm phagocytosis.
Daim duab 02: Kev puas tsuaj ntawm cov protein
Microbial protein degradation nyob rau hauv ib puag ncig tuaj yeem tswj tau los ntawm kev muaj zaub mov. Intracellular protein degradation tuaj yeem ua tiav nyob rau hauv ob txoj kev: proteolysis hauv lysosomes lossis ubiquitin-dependent txheej txheem uas lub hom phiaj tsis xav tau cov proteins rau proteosomes. Txawm li cas los xij, hauv cov protein degradation, cov protein tseem tuaj yeem muaj nws txoj haujlwm lom neeg hauv qee kis.
Dab tsi yog qhov zoo sib xws ntawm Denaturation thiab Degradation ntawm Protein?
- Ob nqe lus yog txuam nrog cov proteins.
- Cov nyiaj cog lus hauv cov protein tau tawg hauv ob txoj kev.
- Lawv ob leeg tseem ceeb heev rau kev ua haujlwm ntawm tes.
- Tus qauv ntawm cov protein ib txwm hloov pauv hauv ob txheej txheem.
Qhov txawv ntawm Denaturation thiab Degradation ntawm Protein yog dab tsi?
Denaturation yog nthuav tawm ntawm cov qauv protein. Qhov ntawd txhais tau tias; poob ntawm nws theem nrab, tertiary lossis quaternary qauv vim raug cuam tshuam rau lub cev lossis tshuaj lom neeg. Tab sis cov qauv tseem ceeb nyob tsis tu ncua vim covalent bonds ntawm cov amino acids muaj zog ntau dua. Txawm li cas los xij, nyob rau hauv degradation ntawm cov proteins, cov qauv tseem ceeb raug rhuav tshem. Qhov ntawd txhais tau tias; covalent bonds ntawm cov amino acids sib txawv yog tawg. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm denaturation thiab degradation ntawm cov protein. Ntxiv mus, nyob rau hauv lub denaturation ntawm cov protein, cov protein poob nws cov kev ua si lom neeg vim hais tias cov hauj lwm yog ncaj qha nyob ntawm nws cov qauv whereas, cov degraded proteins tseem muaj ib tug thib ob los yog tertiary qauv, yog li ntawd lawv tseem muaj peev xwm tseem muaj lawv cov roj ntsha muaj nuj nqi nyob rau hauv tej rooj plaub.
Hauv qab no yog cov ntsiab lus ntawm qhov sib txawv ntawm denaturation thiab degradation ntawm protein nyob rau hauv daim ntawv tabular.
Summary – Denaturation vs Degradation of Protein
Lub denaturation ntawm cov proteins cuam tshuam kev puas tsuaj ntawm ob qho tib si theem nrab thiab qib theem. Tab sis cov qauv tseem ceeb tseem nyob tsis tau. Txawm li cas los xij, hauv kev degradation ntawm cov protein, cov qauv tseem ceeb raug rhuav tshem. Nyob rau hauv lub denaturation ntawm cov protein, cov protein poob nws cov kev ua si lom. Ntawm qhov tod tes, hauv kev degradation, cov proteins tseem muaj lawv cov kev ua haujlwm lom neeg hauv qee kis. Yog li, qhov no qhia txog qhov sib txawv ntawm denaturation thiab degradation ntawm cov protein.
