Qhov sib txawv tseem ceeb ntawm cytosol thiab s9 feem yog tias cytosol yog cov theem ua kua uas muaj cov txheej txheem ntawm lub xovtooj sib nrug ntawm cov nucleus thaum s9 feem yog cov feem ntau supernatant tau los ntawm lub cev homogenate los ntawm qhov qis-ceev centrifugation. nyob rau hauv ib tug haum nruab nrab.
Cytosol thiab S9 feem yog ob feem cuam tshuam nrog cov hlwb. Lub cytosol yog cov kua dej uas cov cell organelles raug tshem tawm. Hauv cov lus yooj yooj yim, nws yog cov theem ua kua uas pom hauv cov ntshav plasma ntawm lub cell. S9 feem yog cov supernatant tau los ntawm qhov qis-ceev centrifugation ntawm cov ntaub so ntswg los yog lub cev homogenate. Nws muaj txiaj ntsig zoo hauv kev tshuaj lom neeg txhawm rau ntsuas cov metabolism ntawm cov tshuaj thiab lwm yam xenobiotics.
YCytosol yog dab tsi?
Cytosol yog ib qho khoom nruab nrab, cov khoom noj muaj txiaj ntsig zoo uas muab thaj chaw rau cov kab mob hauv lub cev thiab lwm cov qauv ntawm tes tshwj tsis yog cov cell nucleus. Sab nrauv ntawm cytosol yog lub plasma membrane. Lub cytosol yog nplua nuj nyob rau hauv cov khoom xws li proteins, carbohydrates, globular qauv, ions, vitamins, thiab minerals. Cov ntsiab lus tseem ceeb tam sim no hauv cytosol yog dej.
Daim duab 01: Cytosol
Cytosol yog nplua nuj nyob rau hauv cov protein ntau vim tias tag nrho cov synthesized proteins muaj nyob rau hauv lub cytosol tom qab kev txhais lus. Tsis tas li ntawd, cytosol tswj cov osmotic tshuav ntawm cov cell thiab pab nws nyob twj ywm. Cytosol kuj tseem pab txhawb kev ua haujlwm ntawm lub cell. Tag nrho cov txheej txheem metabolic tseem ceeb ntawm cov cell tshwm sim hauv cytosol; Yog li ntawd, cytosol yog lub luag haujlwm tseem ceeb ntawm lub cell.
S9 Fraction yog dab tsi?
S9 feem yog cov feem ntau supernatant tau los ntawm thawj qhov qis-ceev centrifugation ntawm cov ntaub so ntswg homogenate. Hauv cov lus yooj yim, nws yog cov khoom lag luam ntawm lub cev nqaij daim tawv homogenate centrifuged ntawm 9000 g rau 20 feeb. Qhov S9 feem no muaj ob qho tib si cytosol (muaj cov proteins soluble) thiab microsomes (membrane proteins). Feem ntau, nws yog siv los ntsuas cov metabolism ntawm cov tshuaj thiab lwm yam xenobiotics. Nws kuj tseem siv tau rau kev kuaj ntshav. Ntxiv mus, S9 feem ntau yog ntxiv rau Ames kuaj los soj ntsuam cov peev xwm mutagenic ntawm cov tshuaj sib xyaw.
Daim duab 02: S9 Fraction
Kev npaj ntawm S9 feem yog ib qho yooj yim thiab nws tau ua tiav los ntawm thawj qhov qis-ceev centrifugation ntawm cov ntaub so ntswg homogenate. Los ntawm kev ua qhov thib ob high-speed centrifugation, cytosol tuaj yeem raug cais tawm ntawm microsomes.
Dab tsi yog qhov zoo sib xws ntawm Cytosol thiab S9 Fraction?
- Cytosol yog ib feem ntawm S9 feem.
- Cytosol tuaj yeem sib cais los ntawm S9 feem los ntawm qhov thib ob high-speed centrifugation.
- Lawv muaj cov proteins soluble.
Qhov txawv ntawm Cytosol thiab S9 Fraction yog dab tsi?
Cytosol yog ib theem ua kua uas muaj cov txheej txheem ntawm cov cell sib nrug ntawm cov nucleus. Nyob rau hauv sib piv, S9 feem yog cov supernatant tau los ntawm cov ntaub so ntswg homogenate los ntawm tsawg-ceev centrifugation. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm cytosol thiab S9 feem. Ntxiv mus, cytosol muaj cov proteins, carbohydrates, globular qauv, ions, vitamins, thiab minerals thaum S9 feem muaj ob cytosol thiab microsomes. Yog li, qhov no kuj yog qhov sib txawv ntawm cytosol thiab S9 feem.
Functionally, cytosol muab thaj tsam ntawm lub cellular organelles thiab lwm yam cellular qauv tshwj tsis yog rau lub cell nucleus. Nws kuj tswj cov osmotic tshuav nyiaj li cas ntawm tes thiab pab cov hlwb kom nyob twj ywm. Hauv qhov sib piv, S9 feem yog siv los ntsuas cov metabolism ntawm cov tshuaj thiab lwm yam xenobiotics. Li no, nws yog qhov sib txawv tseem ceeb ntawm cytosol thiab S9 feem.
Summary – Cytosol vs S9 Fraction
Cytosol yog lub jelly-zoo li matrix uas tsev tag nrho cov txheej txheem organelles ntawm ib lub cell. Nws yog cov khoom siv hauv av uas dais cov txheej txheem organelles xws li ribosomes, mitochondria, thiab chloroplast hauv ib lub cell. Yog li, cytosol yog ib qho nyuaj thiab metabolically active qauv nyob rau hauv lub cell. Hauv qhov sib piv, S9 feem yog cov supernatant tau los ntawm qhov qis-ceev centrifugation ntawm lub cev homogenate. Nws muaj ob qho tib si cytosol thiab microsomes. Qhov no yog qhov txawv ntawm cytosol thiab S9 feem.
