Qhov Tseem Ceeb - Protein A vs Protein G
Kev ua kom huv ntawm IgG cov tshuaj tiv thaiv, lawv cov subclasses thiab lwm hom immunoglobulins (IgA, IgE, IgD thiab IgM) tau ua ntau zaus los ntawm kev siv cov kab mob protein ntau uas muaj kev sib raug zoo rau thaj tsam Fc ntawm cov tshuaj tiv thaiv no. Protein A thiab Protein G yog cov kab mob recombinant proteins uas tau pom zoo rau lub hom phiaj ntawm kev ua kom huv ntawm tib neeg thiab lwm yam tsiaj 'IgG immunoglobulins. Thiab tseem muaj cov protein A, protein G, protein A / G thiab protein L yog cov kab mob microbial recombinant uas muaj qhov tshwj xeeb khi rau thaj tsam Fc ntawm cov tshuaj tiv thaiv kab mob mammalian IgG. Tsis tas li ntawd, cov kab mob microbial no kuj tuaj yeem siv los ntxuav lwm yam immunoglobulin xws li IgA, IgE, IgD, thiab IgM hauv cov tsiaj nyeg thiab lwm yam tsiaj xws li luav, nas, yaj, nyuj thiab lwm yam Protein A khi rau tib neeg cov tshuaj tiv thaiv uas tsis yog IgG antibody. Tab sis nws tsis muaj zog khi nrog tib neeg IgG 3subclass thiab nws tsis khi nrog tib neeg cov tshuaj tiv thaiv IgD. Protein G khi nrog txhua yam subtypes ntawm tib neeg IgG cov tshuaj tiv thaiv kab mob thiab nws muaj ntau yam ntxiv thaum khi rau IgG cov tshuaj tiv thaiv ntawm lwm hom. Txawm li cas los xij, protein G tsis khi rau lwm hom tib neeg cov tshuaj tiv thaiv uas tsis yog IgG. Protein G muaj kev sib raug zoo rau IgG dua li cov protein A. Qhov no yog qhov sib txawv tseem ceeb ntawm cov protein A thiab cov protein G.
YProtein A yog dab tsi?
Protein A yog txhais tias yog cov protein uas nyob hauv qhov loj ntawm 42 kDa. Protein A yog Ameslikas pom nyob rau hauv lub cell phab ntsa ntawm Staphylococcus aureus. Nws yog encoded los ntawm "spa" noob. Protein A yog tswj los ntawm DNA topology, cellular osmolarity, thiab ob lub ntsiab lus. Qhov no microbial recombinant protein ntau koom nrog hauv cov tshuaj biochemical, vim nws muaj peev xwm los khi nrog ntau hom tshuaj tiv thaiv xws li IgG, IgA, IgE, thiab IgM. Yog li, qhov no microbial protein yog siv los ntxuav tib neeg cov tshuaj tiv thaiv kab mob. Nws yog muaj tsib homologous "Ig" binding domains uas quav rau hauv peb-helix bundles. Txhua lub npe muaj peev xwm los khi rau immunoglobulin proteins los ntawm ntau hom tsiaj uas tshwj xeeb tshaj yog nrog IgG cov tshuaj tiv thaiv. Protein A tshwj xeeb khi rau cov saw hnyav ntawm thaj tsam Fc ntawm feem ntau ntawm cov immunoglobulins.
Hais txog tib neeg VH3 tsev neeg cov proteins, Protein A khi rau thaj tsam Fab. Cov recombinant protein A yog dav dua nyob rau hauv nws lub peev xwm los khi rau lwm yam tib neeg cov tshuaj tiv thaiv (IgA, IgE, IgM) uas tsis yog IgG antibody. Tab sis nws tsis muaj zog sib tw nrog tib neeg IgG 3subclass thiab nws tsis khi nrog IgD tib neeg cov tshuaj tiv thaiv. Protein A kuj muaj peev xwm khi rau IgG cov tshuaj tiv thaiv ntawm lwm hom xws li nees, luav, nas, aub, liab, nyuj thiab lwm yam.
Daim duab 01: Protein A
Y Protein A plays lub luag haujlwm tseem ceeb hauv Staphylococcus aureus pathogenesis. Cov protein no txhawb kev sib txuas ntawm cov kab mob mus rau tib neeg Von-Willebrand yam coated nto. Yog li, nws yog ua kom cov kab mob kis tau zoo. Protein A kuj ua rau tib neeg kev sib haum xeeb ntawm kev tiv thaiv kab mob. Qhov no microbial recombinant protein yog tsim los ntawm cov txheej txheem fermentation.
YProtein G yog dab tsi?
Protein G yog txhais tias yog immunoglobulin khi cov protein uas tshwj xeeb qhia los ntawm pawg C thiab D streptococcal kab mob. Nws muaj affinity siab rau Fc thiab Fab cheeb tsam ntawm cov tshuaj tiv thaiv. Protein G muaj kwv yees li 65kDa molecular loj.
YProtein G yog cov protein ntau. Vim nws txoj kev sib raug zoo rau immunoglobulins, nws yog siv rau kev ntxuav cov tshuaj tiv thaiv kab mob. Protein G yog khi nrog txhua hom subtypes ntawm tib neeg IgG cov tshuaj tiv thaiv thiab nws muaj ntau yam ntxiv thaum khi rau IgG cov tshuaj tiv thaiv ntawm lwm hom ib yam nkaus. Tab sis nws tsis khi rau lwm hom tshuaj tiv thaiv tib neeg (IgA, IgE, IgM, IgD). Lub folding ntawm cov protein G - B1 domains nrog rau ib leeg ua rau cov protein globular.
Qhov Zoo sib xws ntawm Protein A thiab Protein G yog dab tsi?
- ob leeg yog cov proteins microbial.
- Ob leeg yog cov proteins sib xyaw.
- Ob leeg cov proteins muaj kev sib raug zoo rau tib neeg IgG antibody thiab nws cov subclasses.
- Ob leeg yog siv rau kev ua kom huv ntawm immunoglobulins.
- Ob leeg cov proteins tuaj yeem khi rau thaj tsam Fc ntawm immunoglobulins.
Qhov txawv ntawm Protein A thiab Protein G yog dab tsi?
YProtein A vs Protein G |
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| Protein A yog txhais tau tias yog 42 kDa qhov loj ntawm cov protein uas yog thawj zaug pom nyob rau hauv phab ntsa ntawm tes ntawm Staphylococcus aureus. | Protein G tau txhais tias yog 65kDa qhov loj me ntawm qhov chaw immunoglobulin khi cov protein uas tshwj xeeb qhia los ntawm pawg C thiab D cov kab mob streptococcal. |
| Qhia hom kab mob | |
| Protein A yog qhia los ntawm Staphylococcus aureus | Protein G yog qhia los ntawm pawg C thiab D cov kab mob streptococcal. |
| Molecular Size | |
| Protein A muaj 42kDa loj. | Protein G muaj kwv yees li 65kDa loj (G148 protein G-65kDa thiab C40 protein G- 58kDa). |
| khi rau Human Serum Albumin | |
| Protein A tsis khi rau cov ntshav albumin. | Protein G muaj qhov chaw khi rau cov ntshav albumin. |
| Kev Ntsig ntawm Tib Neeg IgG 3 Subclass | |
| Protein A tsis tuaj yeem siv rau kev ua kom huv ntawm tib neeg IgG3 subclass vim nws tsis khi rau tib neeg IgG3 subclass immunoglobulin. | Protein G tuaj yeem siv rau kev ua kom huv ntawm tib neeg IgG3 subclass raws li nws khi rau tib neeg IgG3 subclass immunoglobulin. |
| Nruab ntawm Lwm Tib Neeg Cov Tshuaj Tiv Thaiv (IgA, IgE thiab IgM) | |
| Protein A muaj peev xwm ntau dua los khi rau lwm tus tib neeg cov tshuaj tiv thaiv uas tsis yog IgG antibody. Yog li, nws tuaj yeem siv los ntxuav lwm tus tib neeg cov tshuaj tiv thaiv xws li; IgA, IgE thiab IgM. | Protein G yog khi nrog txhua yam subtypes ntawm tib neeg IgG tshuaj tiv thaiv. Tab sis cov protein G tsis khi rau lwm tus tib neeg cov tshuaj tiv thaiv zoo li; IgA, IgE thiab IgM. Yog li, nws tsis tuaj yeem siv los ntxuav lwm cov tshuaj tiv thaiv tib neeg xws li; IgA, IgE thiab IgM |
Summary – Protein A vs Protein G
Cov microbial recombinant protein A, protein G, protein A / G thiab protein L yog cov kab mob hauv cov kab mob uas muaj qhov tshwj xeeb khi rau thaj tsam Fc ntawm cov tshuaj tiv thaiv kab mob mammalian IgG. Protein A thiab protein G kuj muaj qhov chaw khi rau lwm cov tshuaj tiv thaiv IgG ntawm lwm hom. Protein A thiab protein G yog cov kab mob recombinant proteins uas tau pom zoo rau lub hom phiaj ntawm kev ua kom huv ntawm tib neeg thiab lwm yam tsiaj 'IgG immunoglobulins. Protein L muaj affinity siab rau kappa lub teeb chains ntawm immunoglobulin chav kawm IgG, IgA, thiab IgM. Yog li, cov protein L tuaj yeem koom ua ke los ntxuav cov hom immunoglobulin hauv tib neeg thiab lwm hom. Industrially tag nrho cov kab mob no tau siv tam sim no los ntxuav cov immunoglobulins zoo li; IgG, IgA, IgD, IgE, thiab IgM. Qhov no tuaj yeem piav qhia qhov txawv ntawm Protein A thiab Protein G.
Download PDF Version of Protein A vs Protein G
Koj tuaj yeem rub tawm PDF version ntawm kab lus no thiab siv nws rau offline lub hom phiaj raws li daim ntawv ceeb toom. Thov download PDF version ntawm no Qhov txawv ntawm Protein A thiab Protein G
