Qhov sib txawv tseem ceeb ntawm cov noob caj noob ces thiab cov noob cloning yog qhov kev nthuav dav ntawm cov noob yog cov txheej txheem ntawm kev ua ntau cov ntawv luam ntawm cov noob txaus siab hauv vitro los ntawm cov tshuaj tiv thaiv polymerase saw. Tab sis, gene cloning yog txheej txheem ntawm kev ua ntau cov ntawv luam ntawm cov noob ntawm kev txaus siab hauv vivo los ntawm kev ua cov plasmid recombinant thiab hloov nws mus rau hauv cov kab mob.
Ib noob yog ib feem ntawm DNA uas codes rau cov protein. Lub cev muaj ntau txhiab tus noob. Tsis tas li ntawd, nws muaj peev xwm muab cov DNA sib lawv liag ntawm cov noob tseem ceeb uas siv cov txheej txheem lom neeg molecular. Gene amplification thiab gene cloning yog ob txoj hauv kev. Ob txoj kev tsim ntau cov ntawv luam ntawm cov noob paj. Txawm li cas los xij, muaj qhov sib txawv ntawm kev nthuav dav ntawm noob thiab noob cloning raws li lawv txoj kev.
Neeg Amplification yog dab tsi?
Gene amplification yog cov txheej txheem ntawm kev luam cov gene siv polymerase saw cov tshuaj tiv thaiv. Txhawm rau nthuav tawm cov noob, DNA ntawm cov kab mob tshwj xeeb yuav tsum tau muab rho tawm siv cov txheej txheem DNA rho tawm kom raug. Tom qab ntawd cov muab rho tawm ob-stranded DNA yuav tsum tau denatured kom tau cais ib leeg. Cov kauj ruam tom ntej no suav nrog kev siv PCR cov tshuaj tiv thaiv los ua cov ntawv luam ntawm cov noob paj. Tsis tas li ntawd, PCR sib tov muaj cov primers, nucleotides, DNA polymerase enzyme, template DNA thiab lwm yam khoom xyaw. Thaum kawg ntawm PRC qhov kev pab cuam, kev nthuav dav ntawm cov noob ntawm kev txaus siab tshwm sim.
Daim duab 01: Gene Amplification
Tsis tas li ntawd, kev nthuav dav cov noob yog thawj kauj ruam hauv kev tshawb nrhiav cov kab ke tshwj xeeb, kev txheeb xyuas cov kab mob, kuaj kab mob thiab ua cov tsev qiv ntawv noob, thiab lwm yam.
Gene Cloning yog dab tsi?
Gene cloning yog ib txoj hauv kev genetic engineering los ua cov tsev qiv ntawv gene lossis ua ntau cov ntawv luam ntawm cov noob paj. Cov txheej txheem no suav nrog kev ua cov plasmid recombinant thiab hloov pauv ntawm cov plasmid recombinant mus rau hauv ib lub cell, tshwj xeeb tshaj yog cov kab mob cell. Clone yog ib daim qauv zoo tib yam ntawm cov kab mob qub lossis cov molecule. Gene cloning tuaj yeem ua ntau txhiab tus noob clones.
Daim duab 02: Gene Cloning
Thaum cov noob ntawm kev txaus siab raug txheeb xyuas, nws tuaj yeem raug cais tawm ntawm cov genome ntawm ib yam kabmob los ntawm kev txwv nws nrog kev txwv tshwj xeeb enzymes. Tom qab ntawd siv tib qho kev txwv enzyme, plasmids (vectors) yuav tsum tau qhib kom nkag mus rau cov noob caj noob ces. Tom qab ntawd cov plasmid recombinant hloov mus rau hauv cov kab mob uas siv ntau yam xws li electroporation. Qee cov plasmids recombinant nkag mus rau hauv cov kab mob hauv cov kab mob. Siv cov tshuaj tua kab mob, cov kab mob hloov pauv tuaj yeem raug xaiv, cais thiab cog rau hauv cov xov xwm tshiab. Yog li, cov txheej txheem no pab kom tau ntau lab tus noob clones.
Dab tsi yog qhov zoo sib xws ntawm Gene Amplification thiab Gene Cloning?
- Gene amplification thiab gene cloning yog ob txoj kev lom zem molecular.
- Nyob rau hauv ob txoj kev no, ib qho DNA ib ntus lossis cov noob sib npaug hauv lab lossis ntau txhiab zaus.
- Ntxiv mus, ob txoj kev yog qhov tseem ceeb tshaj plaws rau ntau qhov chaw tshawb fawb.
Qhov txawv ntawm Gene Amplification thiab Gene Cloning yog dab tsi?
Gene amplification yog txheej txheem uas ua cov ntawv luam ntawm cov noob tshwj xeeb uas siv hauv vitro txoj kev hu ua PCR. Ntawm qhov tod tes, gene cloning yog txheej txheem uas ua rau cov ntawv luam ntawm ib qho gene siv hauv vivo txoj kev los ntawm kev tsim cov vectors recombinant thiab muab faib rau hauv cov kab mob nyob. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm gene amplification thiab gene cloning. Tsis tas li ntawd, gene amplification yuav siv sij hawm ob peb teev xwb thaum gene cloning yuav siv sij hawm ob peb hnub kom tiav. Yog li ntawd, peb tuaj yeem xav txog qhov no ib yam li qhov sib txawv ntawm cov noob caj noob ces thiab cov noob cloning. Ntxiv mus, cov txheej txheem gene amplification qhia tsawg dua qhov yuam kev, thaum cov txheej txheem gene cloning qhia ntau qhov yuam kev.
Cov infographic hauv qab no qhia txog qhov sib txawv ntawm cov gene amplification thiab gene cloning.
Summary – Gene Amplification vs Gene Cloning
Gene amplification yog cov txheej txheem ntawm kev luam cov noob xav tau siv cov tshuaj polymerase saw cov tshuaj tiv thaiv nyob rau hauv vitro thaum lub noob cloning yog cov txheej txheem ntawm kev luam cov noob ntawm kev txaus siab siv lub recombinant DNA molecule thiab cov kab mob nyob / tus tswv tsev. kab mob. Yog li, qhov no yog qhov sib txawv ntawm cov noob amplification thiab noob cloning.
