Qhov sib txawv tseem ceeb ntawm gene mapping thiab gene sequencing yog tias gene mapping yog cov txheej txheem uas txheeb xyuas qhov chaw ntawm cov noob hauv genome thiab qhov deb ntawm cov noob thaum gene sequencing yog cov txheej txheem uas txheeb xyuas qhov tseeb ntawm nucleotides ntawm ib gene.
Gene mapping and gene sequencing is important in genetic diagnostics to nrhiav thiab paub meej tias muaj cov noob. Yog li, cov txheej txheem molecular xws li gel electrophoresis thiab polymerase saw cov tshuaj tiv thaiv, ua lub luag haujlwm tseem ceeb hauv kev txiav tawm daim ntawv qhia noob. Tsis tas li ntawd, kev siv cov txheej txheem sib txawv xws li Sanger sequencing thiab Next-Generation sequencing yog qhov tseem ceeb los tsim cov noob kab ke.
Gene Mapping yog dab tsi?
Gene mapping yog cov txheej txheem ntawm kev tsim ib daim ntawv qhia tiav ntawm cov chaw ntawm cov noob hauv chromosome. Chromosome mapping yog ib lub ntsiab lus rau gene mapping. Tsis tas li ntawd, cov txheej txheem no muab cov ntaub ntawv hais txog cov noob tshwj xeeb nyob hauv chromosome. Ntxiv mus, gene maping siv txoj kev qhia lub cev thaum lub sijhawm ua daim ntawv qhia.
Daim duab 01: Gene Mapping
Gene mapping tuaj yeem pab nrhiav cov chromosomal aberrations. Yog li ntawd, daim ntawv qhia caj ces ua raws li cov cuab yeej kuaj mob txhawm rau txheeb xyuas cov xwm txheej xws li Downs syndrome thiab Turners syndrome, thiab lwm yam. Karyotyping yog ib qho txheej txheem uas tuaj yeem siv los tsim daim ntawv qhia caj ces. Staining yog ib qho txheej txheem tseem ceeb hauv kev tsim daim ntawv qhia caj ces uas siv cov stains xws li ethidium bromide, acridine txiv kab ntxwv thiab Giemsa. Gene daim ntawv qhia kuj tseem tuaj yeem tsim cov ntawv qhia kev sib txuas uas txheeb xyuas qhov chaw ntawm cov noob caj noob ces tshwm sim.
Dab tsi yog Gene Sequencing?
Gene sequencing yog cov txheej txheem siv los txheeb xyuas thiab txiav txim siab qhov tseeb ntawm tus lej ntawm cov noob. Yog li, los ntawm cov noob caj noob ces, ib tus tuaj yeem txiav txim siab qhov kev txiav txim ntawm adenine, guanine, thymine thiab cytosine bases hauv ib ntu. Thawj tag nrho-genome sequencing tau tshwm sim hauv cov kab mob. Tib neeg genome sequencing project yog ib qho kev hloov pauv ntawm keeb kwm ntawm molecular biology. Tam sim no, gene sequencing plays lub luag haujlwm tseem ceeb hauv kev lag luam thiab kev kho mob. Qhov no qhia meej meej tias muaj cov noob tshwj xeeb hauv genome.
Daim duab 02: Gene Sequencing
Muaj ntau cov txheej txheem los ua kom muaj cov noob. Maxam thiab Gilbert yog cov pioneer ntawm kev qhia txog cov tshuaj siv los ua cov noob caj noob ces lossis DNA. Txawm li cas los xij, kev siv cov tshuaj muaj kev phom sij ua rau kev tso tseg ntawm cov txheej txheem chemical sequencing. Tom qab ntawd, Sanger tau qhia txog cov txheej txheem ua ntu zus uas tsis siv tshuaj lom neeg. Txawm li cas los xij, tam sim no, automated sequencing thiab tiam tom ntej sequencing ua lub luag haujlwm tseem ceeb hauv kev txiav cov kab ke ntawm ib lub noob.
Dab tsi zoo sib xws ntawm Gene Mapping thiab Gene Sequencing?
- Ob txoj kev ua haujlwm tseem ceeb hauv kev kuaj kab mob caj ces.
- Cov tswv yim no muaj peev xwm paub meej tias muaj cov noob hauv cov genome.
- Techniques xws li polymerase saw cov tshuaj tiv thaiv tuaj yeem siv rau hauv ob txoj kev.
Qhov txawv ntawm Gene Mapping thiab Gene Sequencing yog dab tsi?
Qhov sib txawv tseem ceeb ntawm kev txheeb cov noob thiab kev sib txuas ntawm cov noob yog cov ntaub ntawv uas ob txoj kev tsim. Gene mapping muab cov ntaub ntawv ntawm qhov chaw ntawm ib qho gene hauv chromosome thaum gene sequencing generates cov ntaub ntawv hais txog qhov sib lawv liag ntawm lub hauv paus khub ntawm ib tug gene. Txawm li cas los xij, ob txoj kev ua si ua lub luag haujlwm hauv kev ua tus cwj pwm ntawm cov noob. Yog li, lub sijhawm thiab tus nqi them rau kev ua tiav ntawm ob txoj kev sib txawv.
Cov hauv qab no qhia txog qhov sib txawv ntawm gene mapping thiab gene sequencing.
Summary – Gene Mapping vs Gene Sequencing
Gene mapping thiab gene sequencing yog ob txoj kev uas pab ua tus cwj pwm ntawm cov noob. Thaum daim ntawv qhia gene pab nrhiav cov noob, cov noob kab ke muab cov ntaub ntawv biochemical ntawm cov noob tshwj xeeb. Gene mapping thiab gene sequencing yog ib qho tseem ceeb heev nyob rau hauv kev tshawb fawb genetic thiab genetic diagnostics. Tsis tas li ntawd, ob qho tib si cov txheej txheem tam sim no tau siv los ua kom ntseeg tau tias cov txheej txheem tau nrawm thiab raug. Cov ntaub ntawv tsim los ntawm gene mapping thiab gene sequencing yog siv nyob rau hauv downstream txheej txheem thaum lub sij hawm kuaj molecular.
