Qhov Tseem Ceeb Qhov txawv - Gene Sequencing vs DNA Fingerprinting
Kev sib cais ntawm DNA yog cov txheej txheem tseem ceeb hauv cov noob caj noob ces molecular uas cov nucleotide sib lawv liag ntawm DNA ib ntus lossis tag nrho cov genome ntawm lub cev raug txiav txim. Qhov no ua rau tus kws tshawb fawb lossis tus kws tshuaj ntsuam xyuas los txiav txim siab qhov hloov pauv ntawm DNA ib ntus thiab paub qhov txawv ntawm ib lub cev los ntawm lwm tus raws li lawv cov caj ces. Gene sequencing yog cov txheej txheem sequencing ntawm cov noob lossis DNA tawg ntawm Sanger sequencing lossis Next tiam sequencing. DNA ntiv tes suav nrog cov txheej txheem hu ua Restriction Fragment length polymorphism (RFLP), qhov twg DNA cov qauv ntawm ob lossis ntau qhov kev kawm tau tawg thiab txheeb xyuas los txiav txim seb tus neeg yog leej twg. Qhov no yog qhov sib txawv tseem ceeb ntawm cov noob sequencing thiab DNA ntiv tes.
Dab tsi yog Gene Sequencing?
Gene sequencing yog ua los txiav txim seb qhov nucleotide sib lawv liag ntawm ib qho gene. Yog hais tias tag nrho genome yog sequenced, nws yog hu ua Whole Genome sequencing. Thaum pib, kev sib txuas ntawm cov noob tau ua tiav los ntawm kev siv tshuaj lom neeg uas siv cov tshuaj phem xws li Pyridine; Cov txheej txheem no tau raug txiav tawm sai sai vim qhov muaj tshuaj lom ntawm qhov kev sim. Tam sim no, kev sib txuas ntawm cov noob feem ntau yog ua los ntawm txoj kev hu ua Sanger sequencing, uas siv cov saw hlau txiav cov kauj ruam los ntawm deoxyribonucleic acids. Cov tshuaj tiv thaiv yog nqa tawm nyob rau hauv plaub qhov sib cais cov raj uas nyob rau hauv txhua lub raj kuaj cov primer yog sau npe siv ib tug fluorescence marker uas thaum kawg yuav txiav txim seb qhov sib lawv liag ntawm tej yam. Automated Sanger sequencing siv lub ntsuas ntsuas los ntsuas cov teeb liab fluorescence thiab xa cov txiaj ntsig.
Daim duab 01: DNA Sequencing
Tam sim no sequencing yog qhov kev txhim kho tsis ntev los no ntawm cov txheej txheem sequencing thiab yog cov txheej txheem siab dhau los uas sib npaug rau kev ua 1000 Sanger sequencing tshwm sim ib zaug. Cov yam ntxwv tseem ceeb ntawm Next Generation Sequencing yog;
- Highly parallel - ntau qhov kev sib txuas ua ke tshwm sim nyob rau tib lub sijhawm.
- Microscale - cov tshuaj tiv thaiv me me, thiab ntau yam tuaj yeem ua tiav ib zaug ntawm lub nti.
- nrawm - txij li cov kev cuam tshuam tau ua tiav hauv qhov sib npaug, cov txiaj ntsig tau npaj sai dua.
- Ntev ntev - nyeem feem ntau ntawm 505050 -700700700 nucleotides ntev.
Gene sequencing feem ntau yog siv los txiav txim qhov sib lawv liag ntawm cov noob tshiab lossis txhawm rau txheeb xyuas kev hloov pauv ntawm cov noob tam sim no hauv cov kab mob thiab kom paub meej tias cov caj ces ntawm cov kab mob. Nws kuj tseem siv tau rau hauv kev ua liaj ua teb biotechnology los txiav txim siab cov hom tshiab ntawm cov nroj tsuag thiab txheeb xyuas cov noob caj noob ces lub luag haujlwm rau cov txiaj ntsig zoo agronomic xws li kab tsis kam, kab mob tiv thaiv, thiab drought resistance.
DNA ntiv tes yog dab tsi?
DNA ntiv tes yog cov txheej txheem feem ntau siv hauv kev tshawb fawb forensic kom paub meej tias tus kheej ntawm tus neeg koom nrog hauv kev tshawb nrhiav forensic. Thaum ntxov, DNA ntiv tes tau ua tiav siv cov txheej txheem hybridization siv fluorescent lossis xov tooj cua sau cov cim. Tam sim no, DNA ntiv tes yog ua tiav siv cov txheej txheem RFLP. Cov txheej txheem no siv cov enzymes txwv, uas yog cov enzymes muaj peev xwm txiav DNA ntawm cov kab ke tshwj xeeb. Thaum ob qho piv txwv raug coj los tshuaj xyuas, ob qho tib si cov qauv yog zom nrog tib yam kev txwv enzymes los ua cov khoom tawg. Yog tias ob qho piv txwv zoo sib xws, cov duab electrophoresis gel yuav tsum zoo ib yam rau ob qho qauv. Yog tias nws tsis zoo sib xws, cov duab gel yuav tsis zoo ib yam. Yog li, tus kheej ntawm tus neeg tuaj yeem lees paub los ntawm cov txheej txheem no.
DNA ntiv tes feem ntau yog siv los nrhiav tus neeg phem tiag tiag ntawm qhov chaw ua txhaum cai los ntawm kev tshuaj xyuas cov qauv tshuaj lom neeg muaj nyob ntawm qhov chaw ua txhaum cai. DNA tau muab rho tawm los ntawm cov qauv no (cov plaub hau / phev / nti / ntshav) thiab tshuaj xyuas nrog cov qauv DNA ntawm cov neeg raug liam los txiav txim qhov tseeb culprit.
Dab tsi zoo sib xws ntawm Gene Sequencing thiab DNA Fingerprinting?
- Nyob rau hauv ob qho tib si, cov qauv tshuaj ntsuam xyuas yog DNA qauv.
- Electrophoresis yog siv los txiav txim qhov tshwm sim hauv ob txoj kev.
Dab tsi yog qhov txawv ntawm Gene Sequencing thiab DNA Fingerprinting?
Gene Sequencing vs DNA Fingerprinting |
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| Gene sequencing yog cov txheej txheem uas txiav txim siab cov nucleotide sib lawv liag ntawm ib qho gene lossis tag nrho cov genome. | DNA ntiv tes cuam tshuam nrog cov txheej txheem uas DNA kuaj ntawm ob lossis ntau qhov kev kawm tau tawg thiab tshuaj xyuas los txiav txim seb tus neeg yog leej twg. |
| Base of the Technology | |
| Txoj kev sib txuas xws li Sanger sequencing lossis Next Generation sequencing yog siv rau hauv cov noob sequencing. | Txoj Kev Txwv Fragment Length polymorphism yog siv los tshuaj xyuas ob qho piv txwv ntawm kev kawm hauv DNA ntiv tes. |
| Daim ntawv thov | |
| Gene sequencing feem ntau yog siv hauv kev tshawb nrhiav caj ces los tshuaj xyuas cov noob tshiab, txhawm rau txheeb xyuas kev hloov pauv thiab tsim cov kev xav raws li kev tshuaj ntsuam genetic diagnostics. | DNA ntiv tes yog siv rau hauv kev tshawb nrhiav forensic los txiav txim siab txog tus kheej ntawm tus neeg raug liam. |
Summary – Gene Sequencing vs DNA Fingerprinting
Gene sequencing thiab DNA fingerprinting tau dhau los ua ob qhov kev sim nrov uas tau ua los ua tus cwj pwm tshwj xeeb lossis txheeb xyuas tus neeg tshwj xeeb uas siv tus neeg lub caj ces ntiv tes. Cov txheej txheem no yog qhov tseeb thiab nrawm thiab ua los ntawm cov neeg ua haujlwm txawj ntse txhawm rau kom tau txais cov txiaj ntsig tau lees paub. Qhov sib txawv ntawm gene sequencing thiab DNA fingerprinting yog hais tias gene sequencing tsom mus nrhiav qhov tseeb nucleotide kev txiav txim ntawm cov noob thaum DNA ntiv tes tsom rau kev lees paub ntawm tus kheej ntawm cov tib neeg hauv kev tshawb fawb forensic.
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