Qhov sib txawv tseem ceeb ntawm fluorophore thiab chromophore yog tias fluorophore yog cov tshuaj fluorescent tshuaj thaum chromophore tsis yog fluorescent compound. Nws yog ib feem ntawm cov tshuaj sib xyaw uas yog lub luag haujlwm rau cov xim hauv cov molecule.
Muaj ntau daim ntawv thov ntawm fluorophores vim nws muaj peev xwm rov xa tawm lub teeb thaum muaj kev zoo siab uas tshwm sim vim lub teeb ci. Qee daim ntawv thov suav nrog kev siv tshuaj zas lossis staining, ua cov substrate rau cov enzymes, ua cov kua hauv cov kua dej, thiab lwm yam.
YFluorophore yog dab tsi?
A fluorophore yog ib qho tshuaj fluorescent uas tuaj yeem rov ua lub teeb thaum muaj kev zoo siab uas tshwm sim vim lub teeb ci. Cov tebchaw no tau txais cov cuab yeej no vim muaj ntau pawg uas muaj ntxhiab, uas yog sib xyaw ua ke los yog cov qauv sib xyaw ua ke nrog ob peb daim ntawv cog lus pi (ob daim ntawv cog lus). Cov tebchaw no tuaj yeem nqus lub teeb lub zog (ntawm qee qhov wavelength) thiab tuaj yeem rov tso tawm lub zog no raws li qhov ntev wavelength.
Lub wavelengths uas tau nqus los ntawm cov tebchaw no nyob ntawm cov qauv tshuaj fluorophore. Feem ntau, cov tebchaw no me me, cov organic tebchaw, tab sis tuaj yeem muaj cov tebchaw loj dua thiab. Piv txwv li: cov proteins zoo li ntsuab florescent protein.
Daim duab 01: Fluorescence nyob rau hauv UV Radiation
Piv txwv ntawm Flurophore
Qee yam piv txwv rau fluorophores yog raws li hauv qab no:
- Xanthene derivatives xws li fluorescein
- Cyanine
- Naphthalene derivatives
- Coumarin derivatives
- Pyrene derivatives xws li cascade xiav
- YAnthracene derivatives
Chromophore yog dab tsi?
Chromophore yog ib feem ntawm cov molecule, uas yog lub luag haujlwm rau cov xim ntawm cov molecule. Cov cheeb tsam no ntawm cov molecules muaj lub zog sib txawv ntawm ob qho tib si molecular orbitals uas ntog nyob rau hauv lub wavelength ntau yam ntawm pom spectrum. Tom qab ntawd, thaum pom lub teeb hits cheeb tsam no, nws absorbs lub teeb. Qhov no ua rau excitations ntawm electrons los ntawm ib lub xeev hauv av mus rau lub xeev zoo siab. Yog li cov xim uas peb pom yog xim uas tsis absorbed los ntawm chromophore.
Nyob rau hauv cov roj ntsha molecules, chromophore yog ib cheeb tsam uas muaj kev hloov pauv ntawm cov molecule thaum ntaus los ntawm lub teeb. Conjugated pi systems feem ntau ua haujlwm ua chromophores. Lub conjugated pi system muaj ib daim ntawv cog lus thiab ob daim ntawv cog lus nyob rau hauv ib qho kev sib hloov. Cov tshuab no feem ntau tshwm sim hauv cov tshuaj tsw qab.
Qhov txawv ntawm Fluorophore thiab Chromophore yog dab tsi?
A fluorophore yog ib qho tshuaj fluorescent uas tuaj yeem rov ua lub teeb thaum muaj kev zoo siab uas tshwm sim vim lub teeb ci. Chromophore yog ib feem ntawm cov molecule uas yog lub luag haujlwm rau cov xim ntawm cov molecule. Qhov no yog qhov txawv ntawm fluorophore thiab chromophore.
Summary – Fluorophore vs Chromophore
Fluorophores thiab chromophores yog cov tshuaj lom neeg lub luag haujlwm rau pom cov teebmeem hauv cov tebchaw. Qhov sib txawv ntawm fluorophore thiab chromophore yog tias fluorophore yog ib qho tshuaj fluorescent thaum chromophore tsis yog fluorescent compound.
