Plasmid DNA thiab chromosomal DNA yog ob hom DNA muaj nyob hauv cov kab mob. Qhov sib txawv tseem ceeb ntawm plasmid DNA thiab chromosomal DNA yog tias plasmid DNA tsis yog qhov tseem ceeb rau kev muaj sia nyob ntawm cov kab mob thaum chromosomal DNA yog qhov tseem ceeb rau lawv txoj sia nyob vim nws yog genomic DNA ntawm cov kab mob.
Cov kab mob muaj ob hom DNA uas yog chromosomal DNA thiab ntxiv-chromosomal DNA (plasmid DNA). Ob hom yog ncig, ob-stranded DNA. Chromosomal DNA yog genomic DNA ntawm cov kab mob. Nws muaj tag nrho cov noob uas tsim nyog rau kev ciaj sia thiab muaj tag nrho cov ntaub ntawv caj ces rau lawv txoj kev noj qab haus huv. Plasmid DNA muaj cov noob uas muab cov txiaj ntsig zoo ntxiv rau cov kab mob xws li tshuaj tua kab mob, tshuaj tua kab mob, thiab lwm yam.
Plasmid DNA yog dab tsi?
Plasmid DNA yog hom DNA ntxiv-chromosomal hauv cov kab mob. Cov DNA no yog ob-stranded, ncig thiab kaw DNA loops. Lawv raug cais los ntawm genomic DNA ntawm cov kab mob. Lawv tsis muaj cov noob tseem ceeb uas tsim nyog rau kev ciaj sia ntawm cov kab mob. Tab sis lawv muaj cov noob uas muab cov txiaj ntsig zoo ntxiv rau cov kab mob xws li tshuaj tua kab mob, tshuaj tua kab thiab tshuaj tua kab mob, drought kam rau ua, thiab lwm yam. Plasmid DNA muaj keeb kwm ntawm replication. Yog li ntawd, lawv muaj peev xwm rov ua dua tus kheej ntawm nws tus kheej ntawm genomic DNA. Lawv tsis muaj introns; tsis yog lawv coated nrog histone proteins.
Daim duab 01: Plasmid DNA
Vim muaj ntau yam ntawm plasmid DNA xws li kev rov ua dua tus kheej, cov tshuaj tiv thaiv kab mob, thiab lwm yam. lawv muaj lub luag haujlwm tseem ceeb xws li vectors hauv biotechnology. Cov noob tseem ceeb tuaj yeem nkag mus rau cov kab mob ntawm plasmid DNA. Yog li, plasmid DNA muaj daim ntawv thov kev lag luam loj.
Chromosomal DNA yog dab tsi?
Nyob hauv cov kab mob uas muaj sia nyob, genomic DNA tshwm sim raws li chromosomal DNA. Hauv cov kab mob, chromosomal DNA dawb do ntab hauv cytoplasm thaum nyob hauv cov kab mob eukaryotic, lawv nyob hauv lub nucleus. Chromosomal DNA tuaj yeem ua tau ib leeg lossis ob txoj hlua khi. Lawv tuaj yeem yog linear lossis ncig. Qee cov kab mob muaj ob peb chromosomes thaum qee qhov lwm tus, tshwj xeeb tshaj yog cov kab mob thiab archaea, muaj tib chromosome.
Daim duab 02: Chromosomal DNA
Chromosomal DNA muaj tag nrho cov ntaub ntawv caj ces tsim nyog rau kev ciaj sia thiab kev noj qab haus huv ntawm cov kab mob. Cov ntaub ntawv caj ces dhau los ntawm niam txiv mus rau cov xeeb ntxwv ntawm kev rov ua dua ntawm chromosomal DNA. Nws duplicates thaum lub cell faib. Tsis tas li ntawd, chromosomal DNA muaj introns thiab exons. Cov DNA no kuj tau ntim nrog cov proteins histone.
Dab tsi zoo sib xws ntawm Plasmid DNA thiab Chromosomal DNA?
- Ob leeg plasmid DNA thiab chromosomal DNA yog nyob rau hauv cov kab mob.
- Lawv muaj cov noob thiab muaj DNA.
- Lawv tseem ceeb.
Qhov txawv ntawm Plasmid DNA thiab Chromosomal DNA yog dab tsi?
Plasmid DNA yog qhov ntxiv-chromosomal DNA ntawm cov kab mob thaum chromosomal DNA yog genomic DNA ntawm cov kab mob nyob. Plasmid DNA tsis tseem ceeb rau kev ciaj sia ntawm cov kab mob thaum chromosomal DNA tseem ceeb heev rau lawv txoj sia nyob. Qhov no yog qhov sib txawv tseem ceeb ntawm plasmid DNA thiab chromosomal DNA. Ntxiv mus, plasmid DNA me me dua li chromosomal DNA. Cov qub muab cov yam ntxwv ntxiv rau cov kab mob kom ciaj sia nyob rau hauv ib puag ncig hnyav thaum lub sijhawm tom kawg muab tag nrho cov ntaub ntawv rau kev noj qab haus huv ntawm cov kab mob
Tsis tas li ntawd, cov kab mob muaj qhov sib txawv ntawm cov plasmid DNA thaum tsuas muaj ib chromosome hauv cov kab mob. Plasmid DNA yeej ib txwm ncig thaum chromosomal DNA tuaj yeem yog linear lossis ncig. Ntxiv mus, plasmid DNA yeej ib txwm muab tso rau hauv ob qho tib si thaum chromosomal DNA tuaj yeem ua ib leeg lossis ob txoj hlua khi. Cov qub tsis tau ntim nrog histone proteins thaum lub tom kawg yog ntim nrog histone proteins. Plasmid DNA tsis muaj introns lossis cov noob tseem ceeb. Tab sis, chromosomal DNA muaj ob qho tib si introns thiab exons nrog rau tag nrho cov noob tseem ceeb.
Summary – Plasmid DNA vs Chromosomal DNA
Rau qhov xaus, plasmid DNA thiab chromosomal DNA yog ob hom DNA tseem ceeb. Plasmid DNA muab cov yam ntxwv ntxiv rau cov kab mob uas tseem ceeb kom muaj sia nyob hauv cov xwm txheej hnyav. Tsis tas li ntawd, lawv tau siv los ua vectors hauv genetic engineering. Hauv qhov sib piv, chromosomal DNA yog genomic DNA uas muaj cov ntaub ntawv keeb kwm ntawm cov kab mob. Genomic DNA yog ib qho tseem ceeb rau kev ciaj sia ntawm lub cev. Qhov no yog qhov txawv ntawm plasmid DNA thiab chromosomal DNA.
