Qhov sib txawv tseem ceeb ntawm Cosmid thiab Phagemid yog nyob ntawm hom kab ke nws muaj. Lub Cosmid muaj qhov chaw cos thiab plasmid. Yog li ntawd, nws yog hybrid vector thaum Phagemid yog plasmid uas muaj F1 keeb kwm ntawm kev rov ua dua ntawm F1 phage.
Cosmids thiab Phagemids yog siv rau cloning lub hom phiaj, tshwj xeeb tshaj yog los clone loj dua ntawm DNA. Cov no yog siv dav tshwj xeeb tshaj yog nyob rau hauv cloning noob lub luag hauj lwm rau zus tau tej cov proteins. Cosmids thiab Phagemids yog replicated ib leeg li plasmids los yog muab ntim rau hauv cov kab mob thiab ces replicated.
Cosmid yog dab tsi?
A Cosmid, hu ua hybrid plasmid, suav nrog cov chaw cos rho tawm los ntawm Lambda phage hais thiab plasmid. Cov chaw cos no yog cov tawg ntev ntawm DNA nrog 200 basepairs. Li no, lawv muaj cohesive lossis nplaum kawg uas tso cai rau plasmid kom haum rau hauv cov kab mob DNA. Yog li ntawd, cov chaw cos yog qhov tseem ceeb rau kev ntim cov DNA.
Muaj peb qhov chaw cos;
- cosN site - koom nrog nicking DNA strand los ntawm kev ua haujlwm terminase
- cosB qhov chaw - koom nrog tuav lub terminase.
- cosQ site - koom nrog tiv thaiv kev degradation ntawm DNA los ntawm DNases.
Daim duab 01: Cosmid
Tsis tas li, Cosmids tuaj yeem rov ua dua DNA ib leeg lossis ob-stranded DNA siv lub hauv paus tsim nyog ntawm kev rov ua dua. Cosmids feem ntau muaj cov tshuaj tua kab mob tiv thaiv kab mob ua cov cim rau kev xaiv cov hloov pauv. Yog li, kev siv lub cosmid raws li vector tuaj yeem pab txhawb lub cloning, thiab kev txwv enzyme digestion ntawm vector tuaj yeem rho tawm cov fragments.
Phagemid (Phasmid) yog dab tsi?
Phagemid, tseem hu ua Phasmid, yog hom hybrid vector ib yam nkaus. Phagemid muaj lub hauv paus tshwj xeeb ntawm kev rov ua dua lub npe hu ua f1 keeb kwm ntawm kev rov ua dua. Lub hauv paus chiv keeb f1 ntawm replication rho tawm los ntawm f1 phage.
Phagemid tuaj yeem rov ua dua ob qho tib si ib leeg thiab ob txoj hlua DNA. Kev rov ua dua tuaj yeem tshwm sim ua plasmid thaum tab tom ua haujlwm ywj pheej los yog tuaj yeem tau ntim rau hauv phage hais thiab thaum kawg kis tus kab mob E coli. Thaum kis tus kab mob E coli, lub f1 phage yuav tsum muaj lub pilus. Li no, kev sib deev pili yog ib qho tseem ceeb thaum lub sij hawm hauv vitro ntim ntawm Phagemids.
Dab tsi zoo sib xws ntawm Cosmid thiab Phagemid?
- Ob qho tib si Cosmid thiab Phagemid yog cloning vectors siv nyob rau hauv recombinant DNA technology.
- Cosmid thiab Phagemid tuaj yeem rov ua dua ib leeg-stranded thiab ob-stranded DNA.
- Ob leeg tuaj yeem ua raws li kev ywj pheej zoo ib yam li plasmids.
- Cosmid thiab Phagemid tuaj yeem nkag mus rau hauv vitro ntim thiab kis kab mob.
- Ob qho tib si Cosmid thiab Phagemid xav tau lub hauv paus tsim nyog ntawm kev rov ua dua rau cloning.
Qhov txawv ntawm Cosmid thiab Phagmid yog dab tsi?
Cosmid vs Phagemid |
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| Cosmid yog ib tug hybrid vector uas muaj ib cos site thiab plasmid. | Phagemid yog plasmid uas muaj F1 keeb kwm ntawm kev rov ua dua ntawm F1 phage. |
| Ncua Sijhawm Cos | |
| Cos chaw muaj nyob hauv cosmid thiab tsim nyog rau hauv vitro ntim. | Cos qhov chaw tsis nyob hauv phagemid. |
| Muaj F1 Keeb Kwm ntawm Replication | |
| Nyob rau hauv cosmid, fi keeb kwm ntawm replication tej zaum yuav los yog tsis tuaj. | F1 keeb kwm ntawm kev rov ua dua muaj nyob hauv phagemid. |
| Presence of Antibiotic Resistant Genes | |
| Cov noob tshuaj tiv thaiv kab mob muaj nyob hauv cosmids txhawm rau txheeb xyuas cov kev hloov pauv ntawm cov tsis hloov pauv. | Cov tshuaj tiv thaiv kab mob tsis muaj nyob hauv phagemids. |
| Yuav tsum tau ntim hauv vitro | |
| Yuav tsum muaj qhov chaw cos. | Yuav tsum muaj kev sib deev pilus. |
Summary – Cosmid vs Phagemid
Cosmid thiab Phagemid yog cloning vectors siv nyob rau hauv recombinant DNA technology. Cosmids yog hybrid vectors uas muaj cov nplaum nplaum tshwj xeeb hu ua cos sites. ntim hauv vitro xav tau cov chaw cos no. Thaum phagemids yog plasmids uas muaj f1 keeb kwm ntawm replication rho tawm los ntawm f1 phage. Ob qho tib si cosmid thiab phagemid tuaj yeem ua rau muaj kev ywj pheej rov ua dua lossis hauv vitro ntim rau cov kab mob. Cov no yog qhov sib txawv tseem ceeb ntawm cosmid thiab phagemid.
