Qhov Tseem Ceeb Qhov Sib Txawv - Chaw Nres Tsheb vs Txawb Phase
Qhov sib txawv tseem ceeb ntawm qhov chaw nres tsheb thiab lub sijhawm txawb yog qhov chaw nyob ruaj khov tsis txav nrog cov qauv thaum lub sijhawm txawb txav nrog tus qauv.
theem nyob ruaj khov thiab theem txawb yog ob lub ntsiab lus tseem ceeb hauv chromatography, uas yog cov txheej txheem ntawm kev sib cais thiab kev txheeb xyuas cov khoom sib xyaw ua ke.
Phase Chaw Nres Tsheb yog dab tsi?
Stationary theem ntawm cov txheej txheem chromatographic yog qhov sib xyaw siv los cais cov khoom sib xyaw. Txawm li cas los xij, theem no tsis txav mus nrog cov khoom siv. Nws tuaj yeem yog cov khoom sib xyaw lossis cov kua uas txhawb nqa ntawm cov khoom.
Muaj ob hom kev chromatographic loj xws li kem chromatography thiab planar chromatography. Hauv kem chromatography, cov theem nyob ruaj khov yog sau rau hauv ib lub raj hu ua kem. Ntawm no, kem tuaj yeem sau tau ob txoj hauv kev: qee zaum tag nrho kem tau ntim nrog cov theem nyob ruaj khov (lub npe hu ua kem ntim). Lwm lub sij hawm lub kem tau sau nrog rau theem nyob ruaj khov, tawm hauv txoj hauv kev hauv nruab nrab ntawm kem rau kev txav ntawm lub sijhawm txawb (qhib kab kem).
Daim duab 1: Planar chromatography: (1-chromatographic chamber, 2-stationary theem, 3-kua tshuaj pem hauv ntej, 4-mobile theem)
Hauv kev npaj chromatography, ntawm qhov tod tes, kev sib cais yog ua tiav ntawm cov qauv txheej txheem xws li daim ntawv lossis phaj. Lub sijhawm nyob ruaj khov tuaj yeem yog daim ntawv lossis cov kua dej sib koom ua ke ntawm lub phaj. Tsis tas li ntawd, nyias txheej chromatography siv cov ntaub ntawv ua los ntawm cellulose los yog daim hlau siv nrog silica gel. Ntawm no, cov theem nyob ruaj ruaj yog cellulose thiab silica, feem.
Mobile Phase yog dab tsi?
Mobile theem hauv chromatography yog ib qho kev sib xyaw siv los cais cov khoom sib xyaw ua ke. Qhov tseem ceeb tshaj plaws, cov theem no tuaj yeem txav mus nrog cov khoom siv. Yog li ntawd, lub sijhawm mobile khiav los ntawm qhov chaw nres tsheb nrog rau cov qauv. Cov qauv dissolves nyob rau hauv lub mobile theem thiab tsiv mus nyob rau hauv lub nyob ruaj ruaj theem. Lub sijhawm txawb yog kua lossis roj.
Daim duab 2: Gas Chromatography
Piv txwv li, hauv roj chromatography, theem mobile yog roj. Hauv cov kua chromatography thiab ntawv chromatography, cov theem mobile yog cov kua. Lub sijhawm txawb yuav tsum yog qhov hnyav hnyav rau cov qauv. Hauv daim ntawv chromatography, lub sijhawm txawb yuav tsum muaj qhov sib txawv polarity rau cov theem nyob ruaj khov. Qhov no yog vim qhov sib txawv ntawm cov polarities ntawm qhov chaw nres tsheb thiab theem mobile pab cais cov polar, nruab nrab polar thiab nonpolar Cheebtsam hauv kev sib tov.
Qhov txawv ntawm qhov chaw nres tsheb thiab txawb yog dab tsi?
Stationary vs Mobile Phase |
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| Stationary theem ntawm cov txheej txheem chromatographic yog qhov sib xyaw siv los cais cov khoom sib xyaw hauv cov sib tov, tab sis nws tsis txav nrog cov khoom. | Mobile theem nyob rau hauv chromatography yog ib tug compound siv los cais cov Cheebtsam nyob rau hauv ib tug sib tov, thiab nws yuav txav mus nrog rau cov Cheebtsam. |
| Kev tawm mus | |
| Lub sijhawm nyob ruaj khov tsis txav mus. | Them mobile migrates through the stationary phase. |
| Lub sijhawm nyob ruaj khov yog cov khoom sib xyaw lossis cov kua, txhawb nqa ntawm cov khoom. | Lub sijhawm txawb yog roj lossis kua. |
| Qauv Kev Txiav Txim | |
| Lub sijhawm nyob ruaj khov yuav lossis tsis muaj kev cuam tshuam nrog cov khoom hauv cov qauv. | theem txawb kiag li yaj tus qauv. |
Summary – Chaw nres tsheb vs Mobile Phase
Chromatography yog cov txheej txheem biochemical uas cais, txheeb xyuas thiab qee zaum ntsuas cov khoom hauv cov qauv. Cov txheej txheem muaj peb qhov tseem ceeb, uas yog cov qauv, theem nyob ruaj khov thiab theem mobile. Qhov sib txawv tseem ceeb ntawm qhov chaw nres tsheb thiab lub sijhawm txawb yog qhov chaw nres tsheb tsis txav nrog cov qauv thaum lub sijhawm txawb txav nrog cov qauv.
