Qhov Tseem Ceeb Qhov txawv - Isoelectric vs Isoionic Point
Ob nqe lus isoelectric point thiab Isoionic point piav qhia tib lub tswv yim biochemical txog amino acids; lub isoelectric point los yog Isoionic point yog pH uas cov nqi zoo ntawm cov amino acid sib npaug cov nqi tsis zoo ntawm tib cov amino acid. Yog li, tsis muaj qhov sib txawv ntawm cov ntsiab lus isoelectric point thiab Isoionic point.
Isoelectric Point lossis Isoionic Point yog dab tsi?
Isoelectric point lossis Isoionic taw tes ntawm cov amino acid yog pH uas cov nqi zoo ntawm cov amino acid sib npaug ntawm cov nqi tsis zoo ntawm tib cov amino acid. Nws yog denoted los ntawm pI. Txij li thaum tsis muaj cov nqi hluav taws xob hauv cov amino acid, nws tsis tuaj yeem txav mus rau hauv qhov hluav taws xob. Yog li ntawd, isoelectric point yog lub ntsiab lus ntawm cov amino acid yog nruab nrab.
Ntawm isoelectric point, zwitterion yog tsim. Ib zwitterion yog ib qho dipolar molecule uas muaj ntau tshaj ob pawg ua haujlwm muaj cov nqi zoo thiab tsis zoo (cov pab pawg ua haujlwm zoo thiab cov pab pawg tsis zoo). Cov nqi zoo ntawm cov pab pawg ua haujlwm yuav tsum sib npaug rau cov nqi tsis zoo tam sim no ntawm cov pab pawg ua haujlwm ntawm cov amino acid. Qhov no ua rau tus nqi hluav taws xob ntawm lub zwitterion xoom.
Daim duab 01: Cov kua qaub, cov qauv yooj yim thiab nruab nrab ntawm Amino Acid
Ntawm lub ntsiab lus isoionic, zwitterion yog daim ntawv tseem ceeb ntawm cov amino acid. Tus nqi pI tuaj yeem tau txais los ntawm kev siv pKa qhov tseem ceeb ntawm tus nqi zoo thiab cov nqi tsis zoo ntawm cov amino acids hauv cov kua qaub thiab cov nruab nrab nruab nrab.
Yog tias tsis muaj cov pab pawg ua haujlwm nyob rau hauv cov saw hlau ntawm cov amino acid,
pI=½(pKa1 + pKa2) where pKa1 and pKa 2 yog pKa qhov tseem ceeb ntawm cov amino acid nyob rau hauv acidic thiab nruab nrab nruab nrab.
- Yog tias muaj cov kab mob acidic, pI qis dua qhov xav tau.
- Yog tias muaj cov saw hlau yooj yim, pI siab tshaj qhov xav tau.
Qhov txawv ntawm Isoelectric thiab Isoionic Point yog dab tsi?
Isoelectric point, tseem hu ua Isoionic point, ntawm ib qho amino acid yog pH uas cov nqi zoo ntawm cov amino acid sib npaug cov nqi tsis zoo ntawm tib cov amino acid, qhia los ntawm pI
Summary – Isoelectric vs Isoionic Point
Tsis muaj qhov sib txawv ntawm cov ntsiab lus isoelectric point thiab Isoionic point. Ob lub ntsiab lus yog siv los sau npe pH uas tsis muaj hluav taws xob them nyiaj hauv cov amino acid.
