Qhov Tseem Ceeb - Nitrocellulose vs Nylon Membrane
Blotting yog ib qho tseem ceeb txheej txheem rau kev tshawb nrhiav cov kab ke tshwj xeeb ntawm DNA, RNA, thiab cov proteins los ntawm lawv cov kev sib xyaw hauv molecular biology. Nws yog ua tiav siv lub membrane hu ua blot. Muaj ntau hom kev blotting xws li sab qaum teb, sab qab teb thiab sab hnub poob blotting. Xaiv ib daim nyias nyias kom zoo rau cov txheej txheem blotting yuav tsum ua tib zoo ua kom tsis txhob muaj kev sib khi tsis zoo thiab kev kuaj pom tsis raug. Nitrocellulose, nylon, thiab PVDF feem ntau siv daim nyias nyias hauv cov txheej txheem blotting. Lawv muaj cov yam ntxwv sib txawv. Qhov sib txawv tseem ceeb ntawm nitrocellulose thiab nylon membrane yog tias nitrocellulose membranes muaj protein ntau immobilization muaj peev xwm thaum nylon daim nyias nyias muaj peev xwm nucleic acid immobilization siab. Txawm li cas los xij, ob hom membranes feem ntau siv rau hauv cov txheej txheem blotting.
Nitrocellulose Membrane yog dab tsi?
Nitrocellulose daim nyias nyias yog ib qho kev siv cov membrane lim hauv nucleic acid thiab protein blotting cov tswv yim. Nws muaj peev xwm sib txuas cov protein ntau. Li no, nitrocellulose daim nyias nyias yog dav siv nyob rau hauv western blotting txheej txheem. Nitrocellulose daim nyias nyias yog sib xws nrog tag nrho cov txheej txheem hybridization thiab qhia superior binding peev yam tsis muaj kev cuam tshuam. Nitrocellulose membranes yog ib txwm hydrophilic nyob rau hauv cov xwm. Lawv ua hydrophilic kev sib raug zoo nrog molecules thiab zoo immobilize lawv mus rau daim nyias nyias. Kev lag luam nitrocellulose membranes muaj nyob rau hauv ob qhov ntau thiab tsawg: 0.45 thiab 0.2 µm.
Daim duab 01: Nitrocellulose membrane siv rau sab hnub poob blotting
Nylon Membrane yog dab tsi?
Nylon membrane yog lwm hom kev lag luam membrane siv hauv cov txheej txheem blotting. Nws yog lwm txoj kev siv nrog nitrocellulose membranes rau yav qab teb thiab qaum teb blotting. Nylon daim nyias nyias yog zoo tagnrho rau yav qab teb blotting tshaj nitrocellulose vim lawv siab affinity los khi nrog DNA. Vim muaj ntau yam tshwj xeeb ntawm nylon daim nyias nyias, cov kws tshawb fawb feem ntau siv cov nylon daim nyias nyias rau yav qab teb thiab sab qaum teb blotting es tsis txhob tshaj nitrocellulose membrane. Nylon daim nyias nyias kuj pom zoo rau stripping thiab reprobing, tsis zoo li nitrocellulose.
Daim duab 02: Nylon membrane siv rau yav qab teb blotting
Qhov txawv ntawm Nitrocellulose thiab Nylon Membrane yog dab tsi?
Nitrocellulose vs Nylon Membrane |
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| Nitrocellulose membranes yog nkig. | Nylon daim nyias nyias nyias nyias. |
| Lawv nyuaj kho. | Lawv yooj yim daws. |
| Unsupported nitrocellulose membranes nyuaj rau reprobe. | Reprobing yog ib qho yooj yim nrog nylon membranes. |
| Kev sib haum xeeb nrog ntau yam kev cia khoom | |
| Nitrocellulose daim nyias nyias muaj zog tsawg los tiv thaiv ntau yam kev cia khoom. | Nylon membranes teb ntau zog rau ntau yam kev cia khoom. |
| Prewetting yog xav tau. | Prewetting tsis tas yuav tsum tau rau nylon membranes. |
| Hydrophilic Nature | |
| Nitrocellulose daim nyias nyias yog hydrophilic nyob rau hauv qhov tab sis muaj tsawg hydrophilic dua li nylon membranes. | Lawv yog cov dej muaj dej ntau heev. |
| Immobilization Potential | |
| Nitrocellulose daim nyias nyias muaj tsawg affinity rau nucleic acids. Tab sis nws muaj affinity siab rau cov proteins. | Nylon daim nyias nyias muaj peev xwm khi nrog cov nucleic acids dua li nitrocellulose membranes. |
Summary – Nitrocellulose vs Nylon Membrane
Nitrocellulose thiab nylon daim nyias nyias yog cov ntawv tshwj xeeb siv hauv cov txheej txheem blotting los tsim cov qauv banding ntawm cov gel. Lawv pab kom muaj peev xwm kuaj tau cov kab ke tshwj xeeb lossis cov protein los ntawm cov khoom sib xyaw los ntawm immobilizing lawv ntawm daim nyias nyias. Thaum cov molecules immobilized rau ntawm daim nyias nyias, nws tuaj yeem siv los ua lub substrate rau hybridization tsom xam nrog cov ntawv sau npe. Nitrocellulose membrane feem ntau siv rau kev kuaj pom cov protein nyob rau sab hnub poob blotting txheej txheem vim nws txoj kev sib txuas siab nrog cov proteins. Nylon membranes feem ntau siv rau yav qab teb thiab sab qaum teb blotting. Qhov no yog qhov txawv ntawm nitrocellulose thiab nylon membrane.
