Qhov sib txawv tseem ceeb ntawm homologous recombination thiab non-homologous recombination yog tias homologous recombination tshwm sim los ntawm strand ntxeem tau los tsim cov chromosomes recombinant, thaum uas tsis yog homologous recombination yuav siv qhov chaw los ntawm qhov kawg ua kom kaw ob-stranded so.
Recombination yog txheej txheem tseem ceeb rau genomic evolution thiab ntau haiv neeg. Cov txheej txheem los ntawm kev kho DNA puas yog cov txheej txheem ntawm genetic recombination. Homologous recombination muaj ntau txoj hauv kev sib cuam tshuam uas pab kho DNA ob txoj hlua khi thiab cov kab sib txuas sib txuas. Non-homologous recombination yog ib txoj hauv kev uas tseem cuam tshuam nrog DNA kho ob-strand, tshwj xeeb tshaj yog nyob rau hauv siab dua eukaryotes.
Homologous Recombination yog dab tsi?
Homologous recombination yog ib hom kev sib txuas ntawm caj ces uas tshwm sim thaum meiosis. Cov khub chromosomes los ntawm cov txiv neej thiab poj niam cov niam txiv sib haum thaum lub sij hawm homologous recombination kom zoo sib xws DNA sequences los ntawm khub chromosomes hla ib leeg. Qhov no yog hu ua strand invasion. Xws li crossovers ua rau kev sib hloov ntawm cov khoom siv caj ces, ua rau muaj kev hloov pauv caj ces ntawm cov xeeb ntxwv. Homologous recombination feem ntau yog siv los kho cov teeb meem tawg tshwm sim hauv DNA los ntawm cov txheej txheem hu ua homologous recombination kho. Cov kev kho DNA zoo li no yuav ua rau cov khoom tsis sib xws, rov ua kom cov DNA puas ntsoog raws li nws tau ua ua ntej ob txoj hlua tawg.
Daim duab 01: Homologous Recombination
Homologous recombination yog siv thaum lub sijhawm hloov cov noob kab rov tav los pauv cov khoom siv caj ces ntawm ntau hom kab mob thiab kab mob. Homologous recombination yog khaws cia ntawm txhua qhov tseem ceeb nrog rau hauv cov kab mob DNA thiab RNA. Yog li, homologous recombination yog ze li ntawm universal biological mechanism. Qhov no muaj feem cuam tshuam nrog kev ua rau mob qog noj ntshav, kev tsom mus rau noob caj noob ces, thiab kev kho noob. Nws yog ib qho tseem ceeb hauv kev faib cell hauv eukaryotes. Homologous recombination kho DNA puas los ntawm ionizing hluav taws xob los yog tshuaj puas. Ntxiv nrog rau kev kho DNA, nws kuj tseem pab tsim cov noob caj noob ces los ntawm kev faib cov cell meiotic kom dhau los ua cov hlwb tshwj xeeb gamete.
YYam Tsis yog Homologous Recombination yog dab tsi?
Non-homologous recombination yog ib txoj hauv kev uas kho DNA ob txoj hlua khi. Nws yog hu ua non-homologous txij thaum lub so xaus ncaj qha ligate yam tsis xav tau homologous template. Txoj kev no feem ntau yog coj los ntawm cov DNA luv luv hu ua microhomologies. Cov no muaj nyob rau hauv ib leeg-stranded overhangs ntawm qhov kawg ntawm DNA ob-strand so.
Daim duab 02: Tsis yog homologous Recombination
Non-homologous recombination kho qhov so kom raug thaum cov overhangs zoo kawg nkaus sib xws. Qhov tsis tsim nyog uas tsis yog homologous recombination ua rau kev hloov pauv thiab telomere fusion hauv cov qog hlwb. Txoj kev uas tsis yog homologous recombination muaj nyob rau hauv yuav luag tag nrho cov kab mob lom thiab yog qhov tseem ceeb tshaj ob txoj hlua kho txoj hauv kev hauv cov tsiaj. Thaum lub sij hawm inactivation ntawm txoj kev no, ob-strand so yog kho los ntawm ib tug ntau yuam kev txoj kev. Kev kho los ntawm txoj kev no ua rau kev tshem tawm DNA ib ntus ntawm microhomologies. Archaea thiab kab mob tsis muaj txoj hauv kev tsis yog homologous. Hauv qhov sib piv, eukaryotes siv ntau cov proteins thaum tsis yog homologous recombination txoj hauv kev. Qhov no yuav tshwm sim nyob rau hauv cov kauj ruam xws li kawg binding thiab tethering, kawg ua, thiab ligation.
Dab tsi yog qhov zoo sib xws ntawm Homologous Recombination thiab Non-homologous Recombination?
- Homologous thiab non-homologous recombination yog genetic recombinant pathways.
- Ob leeg kho ob txoj hlua khi hauv DNA.
- Recombination tshwm sim ntawm DNA strands thaum ob txoj kev.
- Ntxiv mus, lawv feem ntau tshwm sim hauv eukaryotes.
- Lawv yog ib qho tseem ceeb hauv kev tsom mus rau noob caj noob ces thiab kev kho noob.
Qhov txawv ntawm Homologous Recombination thiab Non-homologous Recombination yog dab tsi?
Homologous recombination yuav siv qhov chaw los ntawm strand ntxeem tau los tsim cov chromosomes recombinant, thaum uas tsis yog homologous recombination tshwm sim los ntawm qhov kawg ua tiav los kaw ob-stranded so. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm homologous recombination thiab non-homologous recombination. Tsis tas li ntawd, homologous recombination tshwm sim ntawm qhov ntev DNA strands thaum uas tsis yog homologous recombination yog coj los ntawm luv DNA sequences. Ntxiv mus, homologous recombination tshwm sim nyob rau hauv eukaryotes, kab mob, thiab cov kab mob thaum uas tsis yog-homologous recombination tshwm sim nyob rau hauv eukaryotes.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm homologous recombination thiab non-homologous recombination nyob rau hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – Homologous vs Non-homologous Recombination
Homologous recombination yog ib hom kev sib txuas ntawm caj ces uas tshwm sim thaum lub sij hawm meiosis nrog rau qhov xav tau rau tus qauv. Meanwhile, non-homologous recombination yog ib txoj hauv kev uas kho DNA ob-strand so. Nws yog hu ua non-homologous txij thaum lub so xaus ncaj qha ligate yam tsis xav tau homologous template. Ntxiv mus, homologous recombination yuav siv sij hawm qhov chaw los ntawm strand ntxeem tau los tsim recombinant chromosomes. Txawm li cas los xij, qhov tsis yog homologous recombination yuav siv qhov chaw los ntawm kev ua tiav kom kaw ob-stranded so. Tsis tas li ntawd, homologous recombination tshwm sim ntawm qhov ntev DNA strands thiab hauv eukaryotes, kab mob, thiab kab mob. Tab sis, tsis yog homologous recombination yog coj los ntawm cov DNA luv luv thiab tshwm sim hauv eukaryotes. Yog li, qhov no qhia txog qhov sib txawv ntawm homologous recombination thiab non-homologous recombination.
