Qhov sib txawv tseem ceeb ntawm avidin thiab streptavidin yog tias avidin muaj kev sib raug zoo thiab tshwj xeeb rau biotin tab sis sib piv tsawg dua li streptavidin vim tias streptavidin-biotin yog ib qho ntawm cov khoom muaj zog tshaj plaws uas tsis muaj cov ntawv cog lus.
Avidin yog ib hom protein uas tsim nyob rau hauv oviducts ntawm noog, tsiaj reptiles, thiab amphibians. Streptavidin yog ib hom protein uas tau npaj los ntawm kev ua kom huv ntawm cov kab mob Streptomyces avidinii.
Avidin yog dab tsi?
Avidin yog ib hom protein uas tsim nyob rau hauv oviducts ntawm noog, tsiaj reptiles, thiab amphibians. Cov protein no yog tetrameric biotin-binding protein uas tso rau hauv cov qe dawb. Muaj cov tswv cuab dimeric ntawm avidin ntxiv rau cov ntawv tetrameric. Peb tuaj yeem pom cov ntawv dimeric no hauv qee cov kab mob. Thaum xav txog qaib qe dawb, nws muaj li ntawm 0.05% ntawm avidin nyob rau hauv kev sib raug rau tag nrho cov protein cov ntsiab lus.
Daim duab 01: Cov qauv tshuaj thiab qhov tshwm sim ntawm Avidin Molecule
Muaj plaub qhov sib npaug ntawm cov tetrameric protein. Yog li ntawd, peb tuaj yeem hu nws ua homotetramer. Txhua tetramer tuaj yeem khi nrog biotin (tseem hu ua vitamin B7). Qhov affinity thiab tshwj xeeb ntawm qhov kev khi no yog siab heev. Avidin-biotin complex yog ib qho ntawm cov paub zoo tshaj plaws uas tsis yog-covalent bonds.
Peb tuaj yeem pom cov haujlwm avidin hauv cov qe nyoos. Thaum siav, biotin affinity rau avidin tau raug puas tsuaj. Txawm li cas los xij, lub ntuj ua haujlwm ntawm lub qe avidin tseem tsis tau paub meej. Cov kws tshawb fawb tau tshaj tawm tias cov protein no tsim nyob rau hauv oviduct ntawm lub qe ua cov kab mob loj hlob inhibitor. Nws tuaj yeem khi nrog biotin (biotin pab tau rau cov kab mob loj hlob).
Ntxiv mus, muaj ib daim ntawv uas tsis yog-glycosylated avidin muaj raws li cov khoom lag luam. Nws tseem tsis tau paub tias daim ntawv no tsis yog glycosylated tshwm sim los yog tsis. Muaj qee qhov kev siv tseem ceeb ntawm cov protein no, suav nrog cov ntawv thov tshawb fawb, tshuaj ntsuam xyuas biochemical, purification media, thiab lwm yam.
Streptavidin yog dab tsi?
Streptavidin yog ib hom protein uas tau npaj los ntawm kev ua kom huv ntawm cov kab mob Streptomyces avidinii. Nws yog tetramer. Muaj homo-tetramers ntawm streptavidin muaj kev sib raug zoo heev rau biotin. Nws paub tias kev khi ntawm biotin rau streptavidin yog ib qho ntawm cov kev sib cuam tshuam tsis zoo tshaj plaws hauv qhov xwm txheej. Cov protein no muaj txiaj ntsig zoo hauv molecular biology thiab bio-nanotechnology vim muaj kev tiv thaiv ntawm streptavidin-biotin complex ntawm cov organic solvents, denaturants, proteolytic enzymes, huab cua kub, pH qhov tseem ceeb, thiab cov tshuaj detergents.
Daim duab 02: Cov qauv thiab qhov tshwm sim ntawm Streptavidin Molecule
Muaj cov ntawv thov tseem ceeb ntawm streptavidin xws li kev ua kom huv lossis tshawb pom ntawm ntau yam biomolecules, purification, thiab nrhiav pom cov noob caj noob ces peptides, hauv Western blotting, tsim Nanobiotechnology, thiab lwm yam.
Qhov txawv ntawm Avidin thiab Streptavidin yog dab tsi?
Avidin yog ib hom protein uas tsim nyob rau hauv oviducts ntawm noog, tsiaj reptiles, thiab amphibians. Streptavidin yog ib hom protein uas tau npaj los ntawm kev ua kom huv ntawm cov kab mob Streptomyces avidinii. Qhov sib txawv tseem ceeb ntawm avidin thiab streptavidin yog tias avidin muaj kev sib raug zoo siab thiab tshwj xeeb rau biotin tab sis sib piv tsis tshua muaj kev sib raug zoo dua li streptavidin vim tias streptavidin-biotin yog ib qho ntawm cov complexes muaj zog uas tsis yog covalent daim ntawv cog lus.
Cov hauv qab no qhia txog qhov sib txawv ntawm avidin thiab streptavidin hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – Avidin vs Streptavidin
Avidin thiab streptavidin yog cov protein tseem ceeb. Qhov sib txawv tseem ceeb ntawm avidin thiab streptavidin yog tias avidin muaj kev sib raug zoo thiab tshwj xeeb rau biotin tab sis sib piv tsis tshua muaj kev sib raug zoo dua li streptavidin vim tias streptavidin-biotin yog ib qho nyuaj tshaj plaws uas tsis muaj cov ntawv cog lus.
