Qhov sib txawv tseem ceeb ntawm T4 DNA ligase thiab E. coli DNA ligase yog T4 DNA ligase yog ib qho enzyme uas cais tawm ntawm bacteriophage T4 thaum E. coli DNA ligase yog ib qho enzyme uas cais tawm ntawm cov kab mob E. coli..
DNA ligase yog ib hom enzyme tshwj xeeb uas pab txhawb kev koom nrog DNA strands ua ke los ntawm kev tsim cov phosphodiester daim ntawv cog lus. Nws ua lub luag haujlwm tseem ceeb los ntawm kev kho ib leeg-strand so thiab ob-strand so hauv DNA ntawm cov kab mob nyob. Ntxiv mus, DNA ligase yog siv nyob rau hauv ob qho tib si DNA kho thiab DNA replication. Sib nrug los ntawm kev siv saum toj no, DNA ligase kuj tseem muaj kev siv dav hauv kev sim tshuaj lom neeg biology xws li recombinant DNA thev naus laus zis. T4 DNA ligase thiab E. coli DNA ligase yog ob hom DNA ligase enzymes.
T4 DNA Ligase yog dab tsi?
T4 ligase yog DNA koom nrog enzyme uas cais tawm ntawm bacteriophage T4. T4 bacteriophage yog tus kab mob uas kis tau Escherichia coli. T4 ligase enzyme yog cov feem ntau siv DNA ligase hauv molecular biological laboratory tshawb fawb. Nquag, T4 ligase catalyzes kev koom tes ntawm ob cohesive los yog blunt-kawg strands ntawm ob-strand DNA ntawm 5 'phosphate thiab 3' hydroxyl pawg ntawm cov nucleotides uas nyob ib sab. Nws tseem tuaj yeem ua rau kev sib koom ua ke ntawm RNA mus rau DNA lossis RNA strand hauv ib lub voj voog duplex. Txawm li cas los xij, cov enzyme no yuav tsis koom nrog ib leeg-stranded nucleic acids. Ntxiv mus, nws tseem tuaj yeem ligate blunt-ended DNA nrog kev ua haujlwm ntau dua li E. coli DNA ligase.
Daim duab 01: T4 DNA Ligase
Qhov enzyme no xav tau ATP ua cofactor. Qee qhov kev tshawb fawb txog kev tshawb nrhiav caj ces twb tau tsim ntau dua DNA ligase enzymes dua li hom tsiaj qus T4 DNA ligase. Hauv ib txoj hauv kev, lawv tsim cov fusion enzyme molecule los ntawm kev sib txuas T4 DNA ligase rau p50 lossis NF-kB molecule. Qhov tshiab T4 ligase enzyme yog 160% ntau dua nyob rau hauv blunt-end ligations rau cloning lub hom phiaj tshaj li qus-hom T4 DNA ligase. Tsis tas li ntawd, qhov zoo tshaj plaws kub rau T4 DNA ligase rau catalyze cov ligation cov tshuaj tiv thaiv ntau precisely yog 16 ° C.
E Coli DNA Ligase yog dab tsi?
E. coli DNA ligase yog ib qho enzyme uas cais tawm ntawm cov kab mob E. coli. Cov noob ua lub luag haujlwm ua E. coli DNA ligase yog lig gene. E. coli DNA, ligase thiab feem ntau prokaryotes DNA ligases, siv lub zog uas tau txais los ntawm NAD (nicotinamide adenine dinucleotide) los tsim cov phosphodiester daim ntawv cog lus.
Daim duab 02: E. Coli DNA Ligase
E. coli DNA ligase yog ib qho ligation enzyme uas tuaj yeem koom nrog DNA fragments los ntawm catalyzing tsim cov phosphodiester bonds ntawm ob-stranded DNA fragments uas muaj 5' phosphate termini thiab 3' hydroxyl termini. Ntxiv mus, nws tsis ligate blunt-ended DNA tshwj tsis yog nyob rau hauv tshwj xeeb tej yam kev mob xws li molecular crowding nrog polyethylene glycol. Nws kuj tsis tuaj yeem koom nrog RNA rau DNA zoo. Tsis tas li ntawd, kev ua haujlwm ntawm E. coli DNA ligase tuaj yeem txhim kho los ntawm qhov muaj DNA polymerase 1 ntawm txoj cai concentration. Txawm li cas los xij, thaum qhov concentration ntawm DNA polymerase 1 enzyme siab dua, nws muaj kev cuam tshuam tsis zoo rau E. coli DNA ligase kev ua haujlwm.
Dab tsi zoo sib xws ntawm T4 DNA Ligase thiab E Coli DNA Ligase?
- T4 DNA ligase thiab E-coli DNA ligase yog ob hom DNA ligase enzymes.
- Ob leeg yog cov proteins ua los ntawm cov amino acids.
- Cov enzymes no ua rau kev tsim cov phosphodiester bond ntawm juxtaposed 5 'phosphate thiab 3' hydroxyl termini hauv duplex DNA.
- Lawv muaj kev siv dav hauv kev sim molecular biology xws li recombinant DNA technology.
Qhov txawv ntawm T4 DNA Ligase thiab E Coli DNA Ligase yog dab tsi?
T4 DNA ligase yog ib qho enzyme uas cais tawm ntawm bacteriophage T4 thaum E. coli DNA ligase yog ib qho enzyme uas cais tawm ntawm cov kab mob E. coli. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm T4 DNA ligase thiab E. coli DNA ligase. Tsis tas li ntawd, lub zog ntawm T4 DNA ligase yog ATP. Ntawm qhov tod tes, lub zog ntawm E. coli DNA ligase yog NAD.
Cov ntawv qhia hauv qab no nthuav tawm qhov sib txawv ntawm T4 DNA ligase thiab E coli DNA ligase hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – T4 DNA Ligase vs E Coli DNA Ligase
T4 DNA ligase thiab E. coli DNA ligase yog ob lub enzymes uas catalyze tsim cov phosphodiester bond ntawm juxtaposed 5' phosphate thiab 3' hydroxyl termini hauv duplex DNA. T4 ligase yog ib qho enzyme uas cais tawm ntawm cov kab mob bacteriophage T4 thaum E. coli DNA ligase yog ib qho enzyme uas cais los ntawm cov kab mob E. coli. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm T4 DNA ligase thiab E coli DNA ligase.
