Qhov sib txawv tseem ceeb ntawm cov cell dawb DNA thiab cov qog qog DNA yog cov cell dawb DNA yog ntau hom DNA uas muaj kev ywj pheej hauv cov ntshav thaum lub qog qog DNA yog qhov tawg ntawm cov qog-derived DNA uas ncig hauv cov ntshav.
Cell free DNA thiab circulating qog DNA yog ob hom circulating nucleic acids. Circulating nucleic acids tau tshawb pom los ntawm Mandel thiab Hlau hauv xyoo 1948. Tom qab ntawd, nws tau tshawb pom tias tus nqi ntawm cov nucleic acids nyob rau hauv cov neeg mob muaj ntau heev. Qhov kev tshawb pom no tau ua thawj zaug hauv kev sib raug zoo rau cov neeg mob lupus. Ntxiv mus, circulating nucleic acids muaj ib tug ntau dua prognostic tus nqi thiab yuav siv tau raws li ib tug biomarker rau nrhiav tau ntau yam kab mob.
Cell Dawb DNA yog dab tsi?
Cell free DNA (Cf DNA) yog hais txog ntau hom DNA xws li cov qog DNA, cell dawb mitochondrial DNA, thiab cell free fetal DNA, thiab lwm yam, uas dawb do nyob rau hauv cov ntshav. Cov qib siab ntawm cov cell dawb DNA tau pom nyob rau hauv cov kab mob siab heev xws li mob qog noj ntshav, kev raug mob, sepsis, myocardial infarction, ntshav qab zib, mob stroke, mob qog noj ntshav, thiab lwm yam. Ntxiv rau cov tshuaj mob qog noj ntshav thiab fetal, cell dawb DNA yog ib qho tseem ceeb biomarker rau ib tug ntau yam mob. Qhov DNA no tuaj yeem siv los txheeb xyuas kev hloov pauv hloov pauv tsis lees paub thiab. Lawv kuj tau siv los txiav txim siab txog kev ntxhov siab, kev sib deev ua ntej yug menyuam, thiab hauv kev sim niam txiv.
Daim duab 01: Cell Dawb DNA
A cell free DNA feem ntau yog ob-stranded extracellular molecule ntawm DNA. Nws muaj cov khoom me me (50 txog 200 bp) thiab cov khoom tawg loj (21 kb). Tsis tas li ntawd, nws twb tau lees paub tias yog biomarker txhim khu kev qha rau kev kuaj mob ntawm prostate thiab mob qog noj ntshav mis. Cell dawb DNA feem ntau ncig hauv cov hlab ntsha li nucleosomes. Nucleosomes yog nuclear complexes ntawm histones thiab DNA. Cf DNA tuaj yeem suav nrog siv ntau yam txuj ci xws li PCR, kev sib txuas loj heev, kev sib txuas ntawm ultraviolet, PicoGreen staining, thiab ELISA. Raws li Cf DNA tshawb pom hauv cov hlab ntsha yog qhov nrawm, yooj yim, tsis cuam tshuam, rov ua dua, yav tom ntej, nws yuav yog lub peev xwm biomarker rau kev kuaj mob ntau yam kab mob xws li autoimmune rheumatic kab mob thiab qog.
Cov qog qog DNA yog dab tsi?
Circulating qog DNA (Ct DNA) yog cov qog nqaij hlav uas tau muab los ntawm DNA uas ncig hauv cov ntshav. Nws muaj cov qog nqaij hlav. Raws li cov qog nqaij hlav tuaj yeem cuam tshuam tag nrho cov qog genome, nws tau txais kev nyiam dav rau nws cov txiaj ntsig zoo hauv kev kho mob. Cov kua dej biopsies, uas kos ntshav, yuav raug siv los kuaj cov qog nqaij hlav.
Daim duab 02: Cov qog nqaij hlav DNA
Cov txheej txheem lom neeg uas muaj feem cuam tshuam rau kev tso tawm Ct DNA suav nrog apoptosis thiab necrosis los ntawm cov hlwb tuag lossis tso tawm cov qog hlwb. Hauv cov ntaub so ntswg noj qab haus huv, infiltrating phagocytes tuaj yeem tshem Ct DNA. Tsis tas li ntawd, ntau yam txuj ci tuaj yeem siv los txheeb xyuas cov qog qog DNA xws li droplet digital PCR, BEAMing, CAPP-Seq (kev sib sib zog nqus sib sib zog nqus), Kev Nyab Xeeb-sequencing, thiab duplex sequencing.
Dab tsi zoo sib xws ntawm Cell Dawb DNA thiab Circulating qog DNA?
- Cell free DNA thiab circulating qog DNA yog ob hom circulating nucleic acids.
- Lawv yog tsim los ntawm nucleotides.
- Ob hom siv tau los ua cov cim cim los kuaj xyuas ntau yam kab mob hauv kev kho mob.
- Lawv pom hauv cov hlab ntsha.
- Ob leeg yog cov ntaub ntawv extracellular ntawm DNA molecules.
- Lawv ob leeg tau tshem tawm los ntawm kev nkag mus rau phagocytes.
Dab tsi yog qhov txawv ntawm Cell Dawb DNA thiab Circulating qog DNA?
Cell free DNA yog ntau hom DNA xws li circulating qog DNA, cell free mitochondrial DNA thiab cell free fetal DNA, thiab lwm yam, uas dawb do nyob rau hauv cov ntshav, thaum circulating qog DNA yog fragmented qog-derived DNA. uas circulates nyob rau hauv cov ntshav. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm cell dawb DNA thiab circulating qog DNA. Tsis tas li ntawd, cell dawb DNA circulates nyob rau hauv fragments xws li 50-220 bp thaum circulating qog DNA circulates nyob rau hauv fragments xws li 134-144 bp.
Cov infographic hauv qab no nthuav tawm qhov sib txawv ntawm cov cell dawb DNA thiab cov qog DNA nyob hauv daim ntawv tabular rau ib sab sib piv.
Summary – Cell Free DNA vs Circulating Tumor DNA
Circulating nucleic acids yog muab pov rau hauv cov hlab ntsha los ntawm kev tswj los yog fortuitous mechanisms. Cell dawb DNA thiab circulating qog DNA yog ob hom circulating nucleic acids. Cell dawb DNA yog ntau hom DNA xws li circulating qog DNA, cell free mitochondrial DNA, cell free fetal DNA uas dawb do circulate nyob rau hauv cov ntshav thaum circulating qog DNA yog lub fragmented qog derived DNA uas circulates nyob rau hauv cov ntshav. Yog li, qhov no qhia txog qhov sib txawv ntawm cov cell dawb DNA thiab cov qog DNA.
