Qhov sib txawv tseem ceeb ntawm EcoRI thiab HindIII txwv enzymes yog EcoRI yog hom II txwv enzyme uas cais tawm ntawm E. coli, thaum HindIII yog hom II txwv enzymes uas cais tawm ntawm Haemophilus influenza.
Kev txwv enzyme lossis txwv tsis pub endonuclease yog ib qho enzyme uas cleaves DNA mus rau hauv cov khoom ntawm cov chaw paub txog (cov chaw txwv) hauv DNA molecule. Cov kev txwv enzymes no muaj nyob rau hauv cov kab mob thiab archaea. Lawv muab cov txheej txheem tiv thaiv tawm tsam cov kab mob invading. Kev txwv enzymes feem ntau muab faib ua tsib hom: hom I, hom II, hom III, IV, thiab hom V. EcoRI thiab HindIII yog ob txoj kev txwv enzymes uas koom nrog pab pawg ntawm hom II txwv enzymes.
EcoRI txwv Enzymes yog dab tsi?
EcoRI yog hom II txwv enzyme uas cais tawm ntawm hom E.coli. Nws yog ib qho kev txwv enzyme uas cleaves DNA ob lub helices rau hauv cov khoom tawg ntawm cov chaw tshwj xeeb. EcoRI kuj yog ib feem ntawm kev txwv-kev hloov kho. EcoRI yog thawj enzyme uas yog thawj zaug cais tawm ntawm RY13 hom kab mob E. coli. Nws yog siv los ua kev txwv enzyme hauv molecular biology. EcoRI tsim 4 nucleotide nplaum xaus nrog 5 'kawg overhangs ntawm AATT. EcoRI txiav cov DNA ntawm qhov kev lees paub tshwj xeeb G↓AATTC. Nws muaj palindromic complementary sequence ntawm CTTAA↓G. Ntxiv mus, qhov txwv tsis pub enzyme belongs rau hom II P (palindromic tshwj xeeb) subclass. Hauv nws cov qauv tseem ceeb, EcoRI muaj PD. D/EXK motif nyob rau hauv nws qhov chaw nquag, zoo li ntau lwm yam txwv enzymes.
Daim duab 01: EcoRI
EcoRI txwv enzyme yog homodimer ntawm 31 kDa. Nws muaj ib tug globular sau ntawm α/β architecture. Txhua lub subunit muaj ib lub voj uas tawm ntawm lub globular sau thiab qhwv ib ncig ntawm DNA thaum khi. Cov enzyme no tuaj yeem cocrystallized nrog cov kab ke nws ib txwm txiav. Tsis tas li ntawd, EcoRI txwv enzyme yog siv nyob rau hauv ntau yam ntawm molecular genetic cov tswv yim, nrog rau cloning, DNA kuaj, thiab deletion seem ntawm DNA in-vitro tej yam kev mob.
HindIII txwv Enzymes yog dab tsi?
HindIII yog hom II txwv tsis pub enzyme uas cais tawm ntawm Haemophilus influenza hom. Nws cleaves DNA palindromic sequence AACCTT nyob rau hauv lub xub ntiag ntawm cov cofactor Mg2+ los ntawm hydrolysis. Qhov kev sib cais ntawm qhov tshwj xeeb no ntawm AA'S (5'A↓ACCTT3' thiab 3'TTCGA↓A5') ua rau 5' overhangs ntawm DNA molecule hu ua nplaum kawg. Cov enzyme no muaj nyob rau hauv cov kab mob prokaryotic zoo li cov kab mob. Cov kab mob siv cov enzyme no los tiv thaiv cov kab mob xws li bacteriophages.
Daim duab 02: HindIII
Structurally, HindIII yog homodimer. Nws muaj cov qauv tsim muaj xws li plaub daim ntawv β thiab ib qho α helices, zoo li lwm hom II txwv enzymes. Molecular mass ntawm no enzyme yog 34.9 kDa. Tsis tas li ntawd, HindIII muaj txiaj ntsig zoo hauv kev sim tshuaj lom neeg niaj hnub molecular xws li DNA sequencing thiab daim ntawv qhia.
Dab tsi yog qhov zoo sib xws ntawm EcoRI thiab HindIII txwv Enzymes?
- EcoRI thiab HindIII yog ob txoj kev txwv enzymes koom nrog hom II txwv enzyme pawg.
- EcoRI thiab HindIII tau pom nyob rau xyoo 1970s.
- EcoRI thiab HindIII ob leeg muaj PD.. D/EXK amino acid sequence motif.
- Ob qho kev txwv enzymes ua haujlwm tshwj xeeb ntawm DNA.
- Cov txwv enzymes xav tau Mg2+ ua cofactor rau lawv cov haujlwm tshwj xeeb.
- Lawv tsim cov nplaum kawg tom qab txiav DNA.
- Lawv yog cov khoom muaj txiaj ntsig zoo hauv kev tshawb fawb molecular biological niaj hnub.
Qhov txawv ntawm EcoRI thiab HindIII txwv Enzymes yog dab tsi?
EcoRI yog hom II txwv enzyme uas cais los ntawm hom E.coli, thaum HindIII yog hom II txwv enzyme uas cais los ntawm Haemophilus influenza hom. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm EcoRI thiab HindIII. Tsis tas li ntawd, EcoRI txiav cov DNA ntawm qhov kev lees paub tshwj xeeb G↓AATTC, thaum HindIII txiav cov DNA ntawm qhov kev lees paub tshwj xeeb A↓ACCTT.
Cov hauv qab no infographic nthuav tawm qhov sib txawv ntawm EcoRI thiab HindIII hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – EcoRI vs HindIII Restriction Enzymes
EcoRI thiab HindIII yog ob txoj kev txwv enzymes uas yog hom II p subclass. Lawv ua qhov tshwj xeeb cleaving ntawm DNA. EcoRI yog hom II txwv enzyme uas cais los ntawm hom E.coli, thaum HindIII yog hom II txwv enzyme uas cais los ntawm Haemophilus influenza hom. Yog li, qhov no qhia txog qhov sib txawv ntawm EcoRI thiab HindIII.
