Qhov sib txawv tseem ceeb ntawm HEK293 thiab HEK293t yog tias HEK293 yog cov kab ntawm tes tsis txawj tuag tau los ntawm lub embryonic tib neeg lub raum uas kis tau los ntawm tib neeg adenovirus hom 5DNA thaum HEK293t yog tus ntxhais cell kab tau los ntawm HEK293 thawj cell kab uas yog transfected nrog plasmid vector nqa SV40 keeb kwm ntawm replication.
Kev hloov pauv feem ntau txhais tau tias yog txheej txheem ntawm kev qhia txawv teb chaws DNA thiab RNA rau hauv cov hlwb txhawm rau cuam tshuam lawv cov genotype thiab phenotype. Xws li kev taw qhia ntawm txawv teb chaws nucleic acids los ntawm ntau yam kev lom neeg, tshuaj, thiab lub cev tuaj yeem hloov pauv cov khoom ntawm tes. Thaum kawg, qhov no tso cai rau kev tshawb fawb ntawm cov noob muaj nuj nqi thiab cov protein qhia hauv cov ntsiab lus ntawm lub cell. HEK293 thiab HEK293t yog ob lub xovtooj ntawm tes uas tau siv dav hauv kev tshawb fawb ntawm tes biology tau ntau xyoo vim tias lawv qhov kev nyiam rau kev hloov pauv.
HEK293 yog dab tsi?
HEK293 yog ib txoj kab ntawm tes tsis txawj tuag tau los ntawm lub embryonic tib neeg lub raum uas kis tau los ntawm tib neeg adenovirus hom 5DNA los tsim ntau cov proteins sib txuas. HEK293s yog Ameslikas cais tawm ntawm tib neeg lub raum hlwb los ntawm Dutch biologist Alex Van der Eb nyob rau hauv 1973. Cov hlwb no tau loj hlob hauv cov ntaub so ntswg kab lis kev cai los ntawm ib tug poj niam fetus. Tom qab ntawd, lawv tau kis los ntawm tib neeg adenovirus hom 5DNA los ntawm Frank Graham, tus kws kho mob tom qab hauv Van der Eb lub chaw kuaj mob. Cov kab ntawm tes no tau hu ua HEK293 vim nws yog Frank's 293rd sim.
Daim duab 01: HEK293
Kev sib koom ua ke ntawm cov noob caj noob ces rau hauv HEK cell genome ua rau cov hlwb no tsim cov protein ntau ntau ua haujlwm tau zoo. Ntxiv mus, adenoviral vector muaj thaj tsam CMV txhawb nqa; yog li ntawd, nws ua rau qhov no efficiency ntxiv. Cov kab ntawm tes no tau siv los ua tus tswv tsev rau kev tshawb fawb gene qhia. Cov kab ntawm tes no kuj tau siv los ntawm kev lag luam biotechnology los tsim cov tshuaj kho cov kab mob rau kev kho noob.
HEK293t yog dab tsi?
HEK293t yog ib tug ntxhais cell kab muab los ntawm HEK293 thawj cell kab uas tau kis nrog plasmid vector nqa SV40 keeb kwm ntawm replication los tsim cov protein ntau recombinant. HEK293t yog tib neeg kab ntawm tes uas qhia txog kev hloov pauv ntawm SV40 loj T antigen. HEK293t tau tsim nyob rau hauv Michele Carlos lub chaw kuaj mob ntawm Stanford los ntawm kev hloov pauv ruaj khov ntawm HEK29E xov tooj ntawm tes nrog plasmid encoding qhov kub-rhiab mutant ntawm SV40 loj T antigen. Nws yog Ameslikas hu ua 293/tsA1609neo. Qhov no yog vim qhov kev hloov pauv tau siv los tsim cov kab ntawm tes uas ua rau muaj kev tiv thaiv neomycin thiab kev qhia ntawm tsA 1609 allele ntawm SV40 loj T antigen.
Daim duab 02: HEK293t
Vim qhov kev qhia ntawm mutant version ntawm SV40 loj T antigen, cov plasmids kis nrog SV 40 keeb kwm ntawm replication tuaj yeem ua rau kom cov protein ntau dua. Cov kab ntawm tes no feem ntau yog siv rau hauv kev lag luam biotechnological rau kev nthuav qhia cov protein thiab kev tsim cov recombinant retroviruses.
Dab tsi yog qhov zoo sib xws ntawmHEK293 thiab HEK293t?
- HEK293 thiab HEK293t yog ob lub xovtooj ntawm tes uas tau siv dav hauv kev tshawb fawb ntawm tes biology vim tias lawv qhov kev nyiam rau kev hloov pauv.
- Ob lub xovtooj ntawm tes yog kuaj tau los.
- Cov kab ntawm tes no tuaj yeem ua rau kom muaj cov protein ntau dua.
- Lawv yog tsim los ntawm kev hloov pauv embryonic tib neeg lub raum hlwb nrog lub plasmid vector.
Qhov txawv ntawm HEK293 thiab HEK293t yog dab tsi?
HEK293 yog cov kab ntawm tes tsis txawj tuag tau los ntawm embryonic tib neeg lub raum uas kis tau los ntawm tib neeg adenovirus hom 5DNA. Nyob rau hauv sib piv, HEK293t yog ib tug ntxhais cell kab muab los ntawm HEK293 thawj cell kab uas yog transfected nrog ib tug plasmid vector nqa SV40 keeb kwm ntawm replication. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm HEK293 thiab HEK293t. Tsis tas li ntawd, HEK293 tsis qhia txog kev hloov pauv ntawm SV40 loj T antigen. Ntawm qhov tod tes, HEK293t nthuav qhia cov mutant version ntawm SV40 loj T antigen.
Cov infographic hauv qab no teev qhov sib txawv ntawm HEK293 thiab HEK293t hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – HEK293 vs HEK293t
HEK293 thiab HEK293t yog ob lub xovtooj ntawm tes uas tau siv dav hauv kev tshawb fawb ntawm tes biology vim tias lawv txoj kev loj hlob tsis tu ncua thiab kev nyiam rau kev hloov pauv. HEK293 yog ib txoj kab uas tsis txawj tuag los ntawm tib neeg lub raum embryonic uas kis tau los ntawm tib neeg adenovirus hom 5DNA, thaum HEK293t yog ib tug ntxhais cell kab tau los ntawm HEK293 thawj cell kab uas kis tau nrog ib tug plasmid vector nqa SV40 keeb kwm ntawm replication. Yog li, qhov no qhia txog qhov sib txawv ntawm HEK293 thiab HEK293t.
