Qhov sib txawv tseem ceeb ntawm cov tshuaj tau hais tseg thiab cov xov xwm nyuaj yog tias cov tshuaj txhais tau tias muaj cov tshuaj lom neeg paub tseeb thaum cov xov xwm nyuaj muaj cov tshuaj tsis paub txog.
Thaum peb loj hlob cov kab mob hauv cov chaw kuaj mob, peb yuav tsum muab tag nrho cov as-ham thiab cov xwm txheej uas lawv xav tau kom tau txais kev loj hlob zoo. Muaj cov xov xwm loj hlob lossis kab lis kev cai tshaj tawm rau lub hom phiaj no. Cov kab lis kev cai nruab nrab yog ib qho khoom, semi-solid lossis kua substrate uas yog nplua nuj nyob rau hauv cov as-ham thiab lwm yam khoom tsim nyog thiab txhawb kev loj hlob ntawm cov kab mob. Peb ua kev cais tawm, txheeb xyuas, thiab saib xyuas cov kab mob hauv kab lis kev cai. Muaj ntau hom kab lis kev cai xov xwm nyob ntawm seb muaj pes tsawg leeg thiab siv. Chemically txhais media thiab complex media yog ob lub ntsiab ntawm cov no. Chemically txhais cov xov xwm muaj cov ntshiab biochemical; li no, chemically txhais cov xov xwm muaj raws nraim paub tshuaj muaj pes tsawg leeg. Ntawm qhov tod tes, cov xov xwm nyuaj muaj cov ntaub ntawv nyuaj xws li ntshav, mis nyuj, nqaij nyug extract, poov xab extract, thiab lwm yam, yog li cov tshuaj muaj pes tsawg leeg ntawm cov xov xwm nyuaj tsis paub.
Yuav ua li cas yog Chemically Defined Media?
Tshuaj txhais cov xov xwm lossis cov khoom siv hluavtaws yog ib hom xov xwm uas muaj cov tshuaj lom neeg paub. Nws yog vim tias hom xov xwm no muaj cov tshuaj dawb huv lossis cov tshuaj uas tau hais tseg. Yog li ntawd, cov tshuaj chemically txhais tau tias muaj cov tshuaj muaj pes tsawg leeg paub tseeb. Cov xov xwm no tsuas yog muab cov khoom noj uas xav tau rau cov microbe rau kev loj hlob. Yog li ntawd, ua ntej yuav siv hom xov xwm no, tus kws tshawb fawb yuav tsum paub txog cov khoom noj khoom haus tiag tiag ntawm cov kab mob uas nws yuav cog rau hauv nruab nrab.
Daim duab 01: Agar Daim phiaj
Tshuaj txhais cov xov xwm muaj txiaj ntsig zoo thaum kawm txog qhov tsawg kawg nkaus ntawm cov kab mob thiab rau ntau yam kev tshawb fawb txog lub cev. Tsis tas li ntawd, cov xov xwm no muaj txiaj ntsig zoo hauv vitro cell kab lis kev cai ntawm tib neeg thiab tsiaj cov hlwb. Glucose ntsev broth yog ib qho piv txwv ntawm xws li xov xwm. Peptone broth yog lwm qhov piv txwv.
Complex Media yog dab tsi?
Complex media yog ib hom xov xwm uas muaj cov ntaub ntawv nyuaj uas yog cov hauv paus chiv keeb xws li ntshav, mis nyuj, poov xab extract, nqaij nyuj extract, thiab lwm yam. Yog li ntawd, cov tshuaj muaj pes tsawg leeg ntawm complex media tsis paub. Txij li cov xov xwm complex muaj cov ntaub ntawv nyuaj, lawv yog cov nplua nuj nyob hauv cov as-ham thiab muab ntau yam kev loj hlob. Yog li, cov xov xwm no pab kom loj hlob cov kab mob uas muaj cov khoom noj khoom haus nyuaj.
Daim duab 02: Ntshav Agar
Tsis tas li ntawd, cov xov xwm nyuaj feem ntau yog siv rau kev cog qoob loo ntawm cov kab mob thiab lwm yam kab mob fastidious. Khoom noj khoom haus broth / agar, tryptic soy broth / agar, thiab ntshav agar yog ob peb yam piv txwv ntawm complex media.
Dab tsi zoo sib xws ntawm Chemically Defined thiab Complex Media?
- Tshuaj txhais cov xov xwm thiab cov xov xwm nyuaj yog ob hom kab lis kev cai uas tso cai rau kev loj hlob ntawm cov kab mob tshwj xeeb tshaj yog cov kab mob.
- Lawv tuaj yeem ua kua, ib nrab lossis cov khoom lag luam.
- Tsis tas li, ob qho tib si xov xwm muaj cov as-ham thiab lwm yam kev loj hlob rau kev loj hlob microbial.
Dab tsi yog qhov txawv ntawm Chemically Defined thiab Complex Media?
Tshuaj txhais tau tias thiab cov xov xwm nyuaj yog ob hom kab lis kev cai tseem ceeb. Chemically txhais cov xov xwm muaj ib tug paub tshuaj muaj pes tsawg leeg thaum complex media muaj ib tug tsis paub hais tias tshuaj muaj pes tsawg leeg. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm chemically txhais thiab complex media. Tsis tas li ntawd, cov tshuaj siv rau kev npaj ntawm cov xov xwm yog vim li cas tom qab paub thiab tsis paub tshuaj muaj pes tsawg leeg. Peb siv cov tshuaj biochemical ntshiab los npaj cov tshuaj chemically txhais cov ntaub ntawv thiab cov ntaub ntawv nyuaj xws li ntshav, mis nyuj, nqaij nyug extract, poov xab extract los npaj complex media.
Cov infographic hauv qab no muab cov ntsiab lus ntxiv ntawm qhov sib txawv ntawm cov tshuaj uas tau hais tseg thiab cov xov xwm nyuaj sib piv.
Summary – Chemically Txhais thiab Complex Media
Nyob rau hauv cov ntsiab lus ntawm qhov sib txawv ntawm chemically txhais thiab complex media, chemically txhais cov xov xwm muaj ib tug paub tshuaj muaj pes tsawg leeg, tab sis complex media muaj ib tug tsis paub hais tias tshuaj muaj pes tsawg leeg. Qhov ntawd yog; cov chemically txhais tau tias yog cov tshuaj dawb huv/paub cov tshuaj thaum complex media constitutes complex cov ntaub ntawv xws li ntshav, mis nyuj, poov xab extract, nyuj extract, thiab lwm yam. Yog li ntawd, qhov no yog qhov tseem ceeb sib txawv ntawm chemically txhais thiab complex media. Tsis tas li ntawd, chemically txhais cov xov xwm yog cov xov xwm me me uas muab cov khoom noj khoom haus tiag tiag ntawm cov kab mob thaum cov xov xwm nyuaj yog cov kab lis kev cai nplua nuj uas muab tag nrho cov as-ham thiab lwm yam kev loj hlob rau cov kab mob.
