Qhov sib txawv tseem ceeb ntawm organoids thiab spheroids yog tias organoids yog 3D cell kab lis kev cai uas feem ntau loj hlob ntawm scaffold-raws li system, thaum spheroids yog 3D cell kab lis kev cai uas loj hlob ntawm ib tug scaffold-free system.
Organoids thiab spheroids yog ob hom 3D cell kab lis kev cai. 3D kab lis kev cai ntawm tes yog ib pawg ntawm cov kab mob lom neeg loj hlob hauv ib puag ncig tsim khoom tsim. Cov kab mob lom no tau tso cai kom loj hlob lossis cuam tshuam nrog lawv ib puag ncig hauv txhua qhov peb. 3D cell kab lis kev cai tau muab faib ua ob hom: scaffold-based system thiab scaffold-free system. Scaffold-based system siv cov khoom siv ntuj tsim los yog cov khoom siv hluavtaws los ua kev txhawb nqa rau cov noob hlwb kom sib sau ua ke, proliferate, thiab migrate. Nyob rau hauv qhov sib piv, ib tug scaffold-free system txhawb kev sib sau ntawm tus kheej ntawm cov kab lis kev cai tshwj xeeb los yog lub cev tsis muaj zog uas tiv thaiv kev sib txuas ntawm tes.
Organoids yog dab tsi?
Organoids yog 3D kab lis kev cai ntawm tes uas nquag loj hlob ntawm cov kab ke raws li scaffold. Lawv yog cov qauv me me ntawm cov kabmob loj hlob hauv vitro hauv peb qhov ntev. Lawv qhia qhov tseeb microanatomy. Organoids feem ntau yog muab los ntawm ib tus neeg laus qia cell los yog embryonic qia cell. Lawv kuj tuaj yeem tsim los ntawm induced pluripotent qia hlwb xws li tawv nqaij lossis cov qe ntshav. Cov hlwb no tuaj yeem sib sau ua tus kheej thaum lawv muab scaffolding extracellular ib puag ncig xws li Corning® Matrigel® matrix ntawm collagen. Thaum kawg, lawv loj hlob mus rau hauv microscopic versions ntawm niam txiv lub cev uas siv tau rau kev tshawb fawb 3D. Cov txheej txheem ntawm kev tsim cov organoids sai tau txhim kho txij li xyoo 2010. Cov ntawv xov xwm Science tau hu cov txheej txheem no ua ib qho kev nce qib loj tshaj plaws ntawm xyoo 2013. Cov kev siv tseem ceeb ntawm organoids yog los kawm cov kab mob thiab kev kho mob hauv cov chaw sim.
Daim duab 01: Tsim cov Cerebral Organoids
Tsis tas li ntawd, qee qhov piv txwv ntawm organoids yog cerebral organoids, plab organoid, plab hnyuv organoid, plab los yog gastric organoid, lingual organoid, thyroid organoid, thymic organoid, testicular organoid, thiab hepatic organoid..
Spheroids yog dab tsi?
Spheroids yog 3D kab lis kev cai ntawm tes uas tau loj hlob ntawm lub tshuab tsis muaj scaffold. Lawv muaj cov cell aggregates tsim los ntawm ib hom ntawm tes los yog los ntawm ntau cov cellular sib xyaw. Spheroids tuaj yeem tsim los ntawm cov kab tsis txawj tuag, cov hlwb tseem ceeb, lossis cov ntaub so ntswg ntawm tib neeg. Lawv yog cov yooj yim pawg ntawm cov hlwb dav xws li lub cev embryoid, hepatocytes, qog nqaij hlav, cov ntaub so ntswg, lossis cov qog mammary.
Daim duab 02: Spheroids
Spheroids tsis tas yuav scaffolding los tsim 3D cell kab lis kev cai. Nyob rau hauv cov spheroids no, cov kab lis kev cai tsis tshua muaj adhesion tuaj yeem siv los txhawb kev sib sau ua ke ntawm cov hlwb rau hauv sphere-shaped 3-dimensions. Lawv ua li ntawd los ntawm ib txwm ua rau ib leeg. Spheroids kuj tuaj yeem siv los siv lwm txoj kev sib txawv xws li txoj kev dai dai thiab tig phab ntsa hlab ntsha bioreactors. Txawm li cas los xij, lawv tsis tuaj yeem sib sau ua ke lossis rov tsim dua. Yog li, spheroids tsis zoo li organoids.
Dab tsi yog qhov zoo sib xws ntawm Organoids thiab Spheroids?
- Organoids thiab spheroids yog 3D cell kab lis kev cai.
- Ob leeg yog cog hauv vitro kuaj mob.
- Lawv yog cov kab lis kev cai ntawm tes.
- Ob leeg tuaj yeem tsim los ntawm ntau yam ntaub so ntswg noj qab haus huv nrog rau cov kab mob ntawm tes thiab cov ntaub so ntswg xws li qog.
- Lawv siv los kawm txog kab mob thiab kev kho mob.
Qhov txawv ntawm Organoids thiab Spheroids yog dab tsi?
Organoids yog 3D cell kab lis kev cai uas feem ntau loj hlob ntawm scaffold-raws li system, thaum spheroids yog 3D cell kab lis kev cai uas loj hlob nyob rau hauv ib tug scaffold-dawb system. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm organoids thiab spheroids. Tsis tas li ntawd, organoids tuaj yeem sib sau ua ke thiab rov tsim dua tshiab, whereas spheroids tsis tuaj yeem sib sau ua ke thiab rov tsim dua tshiab.
Daim duab hauv qab no teev qhov sib txawv ntawm organoids thiab spheroids hauv daim ntawv tabular rau ib sab ntawm kev sib piv.
Summary – Organoids vs Spheroids
Cov kab lis kev cai 3D cell yog ib puag ncig kab lis kev cai uas tso cai rau cov hlwb loj hlob thiab cuam tshuam nrog lawv cov txheej txheem nyob ib puag ncig hauv peb qhov ntev. Organoids thiab spheroids yog ob hom 3D cell kab lis kev cai. Organoids yog 3D cell kab lis kev cai uas feem ntau zus nyob rau hauv ib tug scaffold-raws li system, thaum spheroids yog 3D cell kab lis kev cai uas yog zus nyob rau hauv ib tug scaffold-free system. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm organoids thiab spheroids.
