Qhov sib txawv ntawm DNA thiab protein microarray yog DNA microarray yog ib qho kev sib sau ntawm cov kab mob DNA microscopic txuas rau cov khoom nto zoo li iav swb, thaum cov protein microarray yog kev npaj ntawm cov protein uas huv si ntawm cov khoom zoo li iav. swb.
Microarray yog lub chaw kuaj mob ntawm lub nti (LOC) ntaus ntawv. Nws yog ib qho me me, multiplex, kev sib txuas ua ke thiab nrhiav kom pom cov cuab yeej. Nws kuj tseem yog ob-dimensional array ntawm cov khoom nto xws li iav swb lossis silicon nyias-film cell. Lub microarray soj ntsuam ntau cov khoom siv roj ntsha uas siv cov kev tshuaj ntsuam xyuas siab. Thawj microarray yog cov tshuaj tiv thaiv kab mob uas tau qhia los ntawm Tse Wen Chang xyoo 1983. Thawj cov noob nti tau qhia hauv xyoo 1995 los ntawm Ron Davis thiab Pat Brown ntawm Stanford University. Yog li ntawd, DNA thiab protein microarray yog ob hom microarray li.
DNA Microarray yog dab tsi?
DNA microarray yog ib qho kev sau ntawm cov kab mob DNA microscopic txuas rau ntawm qhov chaw zoo li iav swb. Cov kws tshawb fawb siv DNA microarray los ntsuas qhov kev qhia ntawm ntau cov noob ib txhij. Nws kuj tseem siv los ua genotype ntau thaj tsam ntawm genome. DNA microarray yog npaj siv cov tshuab robotic. Cov tshuab no npaj ntau pua lossis ntau txhiab tus kab ke ntawm cov noob (probes) ntawm ib qho chaw me me.
Daim duab 01: DNA Microarray
Hauv DNA microarray, txhua qhov DNA muaj picomoles (10 −12moles) ntawm ib ntu DNA tshwj xeeb. Cov DNA sequences no tseem hu ua probes lossis reporters. Cov kev sojntsuam no yog ntu luv ntawm DNA ntawm cov noob lossis lwm yam DNA. Lawv tuaj yeem hybridize nrog cDNA lossis CRNA hauv cov qauv hauv cov xwm txheej siab. Cov fluorescently sau cov qauv ua ntu zus uas khi rau kev sojntsuam tsim cov teeb liab uas nyob ntawm hybridization tej yam kev mob xws li kub. Yog li ntawd, txoj kev no tuaj yeem siv los txiav txim siab qhov sib npaug ntawm nucleic acid sequences hauv cov qauv. Ntxiv mus, cov txheej txheem DNA microarray tau siv dav hauv cov kev tshawb fawb txog noob caj noob ces, kev sib piv cov kev tshawb fawb genomic, kuaj xyuas cov kab mob sib kis ntawm cov zaub mov lossis kab lis kev cai ntawm tes los ntawm cov kab mob, ntsuas cov noob hloov pauv, txheeb xyuas cov tshuaj sib tw, tshuaj ntsuam genetic linkage, thiab lwm yam.
Protein Microarray yog dab tsi?
Protein microarray yog ib qho kev npaj ntawm purified proteins ntawm ib tug khoom nto zoo li iav swb. Nws yog ib txoj hauv kev siv los txheeb xyuas kev sib cuam tshuam thiab kev ua haujlwm ntawm cov protein. Nws kuj tseem siv los txiav txim siab txog kev ua haujlwm ntawm cov proteins ntawm qhov loj. Lub ntsiab kom zoo dua ntawm cov protein microarray yog tias ntau cov proteins tuaj yeem taug qab ua ke. Protein microarray yog ib nti uas muaj qhov chaw txhawb nqa zoo li iav swb, nitrocellulose membrane, hlaws lossis microtitre phaj. Cov proteins uas ntes tau (antibodies, antigens) yog khi rau qhov khoom no. Cov tshuaj ntsuam xyuas molecules feem ntau sau nrog cov xim fluorescent ntxiv tom qab rau hauv cov array. Lub hybridization ntawm kev sojntsuam thiab immobilized proteins emits lub teeb liab fluorescent uas tuaj yeem kuaj pom los ntawm lub laser scanner.
Daim duab 02: Protein Microarray
thawj cov protein array yog cov tshuaj tiv thaiv kab mob microarray (antibody matrix) uas tau qhia hauv xyoo 1983. Protein microarrays yog ceev, automated, rhiab heev, thiab kev lag luam. Lawv kuj tsuas yog siv me me ntawm cov qauv thiab cov tshuaj reagents. Tsis tas li ntawd, cov protein microarrays tau siv dav rau kev kuaj mob, kev tsom xam cov protein ua haujlwm, tshuaj tiv thaiv tus yam ntxwv, thiab kev kho mob.
Dab tsi zoo sib xws ntawm DNA thiab Protein Microarray?
- DNA thiab protein microarray yog ob hom microarray.
- Ob qho khoom siv yog raws li txoj cai hybridization.
- Ob lub cuab yeej siv kev sojntsuam.
- Lawv kuj siv cov teeb liab fluorescence txhawm rau txhawm rau txheeb xyuas hybridization.
- Lawv ob leeg muaj txiaj ntsig zoo hauv kev kuaj kab mob hauv tshuaj.
Qhov txawv ntawm DNA thiab Protein Microarray yog dab tsi?
DNA microarray yog ib qho kev sau ntawm cov kab mob DNA microscopic txuas rau ntawm qhov chaw zoo li iav swb. Nyob rau hauv sib piv, protein microarray yog ib qho kev npaj ntawm purified proteins ntawm ib tug khoom nto zoo li ib tug iav swb. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm DNA thiab protein microarray. Tsis tas li ntawd, hauv DNA microarray, cov qauv thiab kev sojntsuam yog DNA sequences. Ntawm qhov tod tes, hauv cov protein microarray, cov qauv thiab kev sojntsuam yog cov proteins.
Cov infographic hauv qab no nthuav tawm qhov sib txawv ntawm DNA thiab protein microarray hauv daim ntawv tabular.
Summary – DNA vs Protein Microarray
Ib microarray yog qhov ua tau zoo tshaj plaws thiab multiplex lab-on-a-chip. Nws yog tsim los ntawm txoj cai hybridization. DNA thiab protein microarray yog ob hom microarray li. DNA microarray yog ib qho kev sau ntawm microscopic DNA me ntsis txuas rau ntawm qhov chaw zoo li lub iav swb. Ntawm qhov tod tes, protein microarray yog ib qho kev npaj ntawm purified proteins ntawm ib tug khoom nto zoo li ib tug iav swb. Yog li, qhov no yog cov ntsiab lus ntawm qhov sib txawv ntawm DNA thiab protein microarray.
