Qhov sib txawv tseem ceeb ntawm homologous recombination thiab qhov chaw tshwj xeeb recombination yog tias nyob rau hauv homologous recombination, genetic khoom yog pauv ntawm ob zoo tib yam molecules ntawm ob-stranded los yog ib-stranded nucleic acids xws li DNA los yog RNA, whereas nyob rau hauv site- Kev sib koom ua ke tshwj xeeb, DNA kev sib pauv hloov pauv tshwm sim ntawm cov ntu DNA uas muaj tsawg kawg yog qee qhov kev sib luag ntawm homology tab sis tsis muaj homology dav dav.
Recombination yog txheej txheem los ntawm cov khoom ntawm DNA tau tawg thiab rov ua dua los tsim cov kev sib txuas tshiab ntawm alleles. Cov txheej txheem no tsim muaj kev sib txawv ntawm cov kab mob sib txawv. Nws tseem hu ua genetic reshuffling. Nws txhais tau tias yog cov txheej txheem uas kev sib pauv ntawm cov khoom siv caj ces ntawm cov kab mob sib txawv tshwm sim los tsim cov xeeb ntxwv nrog cov kev sib txuas tshiab ntawm alleles uas txawv ntawm cov uas pom hauv niam txiv. Homologous recombination thiab site-specific recombination yog ob hom recombination mechanisms.
Homologous Recombination yog dab tsi?
Homologous recombination yog ib hom kev sib txuas ntawm caj ces nyob rau hauv uas cov khoom siv caj ces sib pauv ntawm ob qhov zoo sib xws (zoo tib yam) molecules ntawm ob-stranded lossis ib leeg-stranded nucleic acids (DNA lossis RNA). Nws yog dav siv los ntawm cov hlwb los kho cov teeb meem tawg uas tshwm sim hauv ob qho tib si DNA strands, hu ua double-strand so (DSB). Cov txheej txheem no hu ua homologous recombinational repair (HRR). Tsis tas li ntawd, homologous recombination nyob rau hauv eukaryotes tsim tshiab ua ke ntawm DNA sequences thaum lub sij hawm meiosis. Meiosis yog cov txheej txheem uas eukaryotes ua gametes xws li phev thiab qe qe.
Daim duab 01: Homologous Recombination
Homologous Recombination Mechanism
Thaum meiosis, khub chromosomes los ntawm txiv neej thiab poj niam niam txiv sib dhos kom cov DNA zoo sib xws los ntawm khub chromosomes muaj lub sijhawm hla ib leeg. Qhov kev hla dhau no ua rau kev sib hloov ntawm cov khoom siv caj ces. Homologous recombination tshwm sim nrog kev pab los ntawm cov proteins xws li PRDM9, SPO11, DMC1, ZCWPW1, RPA, Dna2, BLM, CtIP, BRCA1, BRCA2, thiab lwm yam. Cov kev sib txuas tshiab ntawm DNA lossis alleles tsim kev hloov pauv hauv cov xeeb ntxwv. Nws ua rau cov pejxeem hloov pauv thaum lub sijhawm hloov pauv.
Homologous recombination kuj siv los ntawm cov kab mob thiab cov kab mob. Qhov no hu ua "kev hloov cov noob kab rov tav", ib txoj kev sib pauv cov khoom siv caj ces ntawm cov kab mob sib txawv thiab hom kab mob thiab kab mob.
Qhov Chaw-Specific Recombination yog dab tsi?
Site-specific recombination yog hom caj ces recombination nyob rau hauv uas DNA strand pauv tshwm sim ntawm DNA segments muaj tsawg kawg yog ib tug tej yam degree ntawm sequence homology tab sis tsis muaj nws kim heev homology. Nws tshwm sim hauv cov kab mob genome replication, sib txawv pathogenesis, thiab txav ntawm cov noob caj noob ces.
Site-specific Recombination Mechanism
Enzymes uas paub tias qhov chaw tshwj xeeb recombinases (SSRs) tau koom nrog hauv cov txheej txheem no. Lawv paub thiab khi rau luv luv thiab tshwj xeeb DNA sequences. Tom qab ntawd lawv txiav cov pob txha DNA thiab pauv ob DNA helices koom nrog. Thaum kawg, cov enzymes no rov koom nrog DNA strands.
Daim duab 02: Qhov Chaw-Specific Recombination
Feem ntau, muaj cov enzyme recombinase thiab recombination chaw txaus rau cov txheej txheem no. Tab sis qee zaum, yuav tsum muaj cov khoom siv ntxiv protein lossis cov khoom siv ntxiv. Qhov chaw tshwj xeeb recombination systems yog qhov tshwj xeeb, nrawm, thiab ua haujlwm tau zoo. Yog li ntawd, lawv yog cov cuab yeej muaj peev xwm rau genetic engineering.
Dab tsi yog qhov zoo sib xws ntawm Homologous Recombination thiab Site-Specific Recombination?
- Lawv yog hom kev sib txuas ua ke.
- Lawv txhim kho caj ces txawv ntawm cov kab mob sib txawv.
- Ob yam txheej txheem tshwm sim ntawm DNA.
- Lawv siv cov proteins tshwj xeeb rau kev sib txuas ua ke.
- Ob leeg coj qhov chaw hauv prokaryotes thiab hauv eukaryotes.
Qhov txawv ntawm Homologous Recombination thiab Site-Specific Recombination yog dab tsi?
Homologous recombination yog ib hom kev sib txuas ntawm caj ces nyob rau hauv uas cov khoom siv caj ces pauv ntawm ob qho tib si molecules ntawm ob-stranded los yog ib leeg-stranded nucleic acids xws li DNA lossis RNA. Ntawm qhov tod tes, Site-specific recombination yog ib hom kev sib txuas ntawm cov noob caj noob ces nyob rau hauv uas DNA strand pauv tshwm sim ntawm DNA ntu uas muaj tsawg kawg yog qee qhov kev sib luag ntawm homology tab sis tsis muaj homology dav dav. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm homologous recombination thiab site-specific recombination. Ntxiv mus, homologous recombination tshwm sim ntawm ntev DNA strands. Nyob rau hauv sib piv, qhov chaw tshwj xeeb recombination tshwm sim ntawm luv DNA sequences. Yog li, qhov no yog lwm qhov sib txawv tseem ceeb ntawm homologous recombination thiab site-specific recombination.
Hauv qab no yog cov npe ntawm qhov sib txawv ntawm homologous recombination thiab site-specific recombination nyob rau hauv daim ntawv tabular.
Cov ntsiab lus sib piv – Homologous vs Site-Specific Recombination
Kev tshuaj ntsuam genetic recombination suav nrog kev sib pauv ntawm cov khoom siv caj ces ntawm ntau tus chromosomes lossis ntawm thaj chaw sib txawv ntawm tib chromosome. Homologous recombination thiab site-specific recombination yog ob hom recombination mechanisms. Homologous recombination tshwm sim ntawm DNA nrog nws cov kab ke ntau homology. Qhov chaw tshwj xeeb recombination tshwm sim ntawm DNA tsis muaj homology dav dav. Yog li, qhov no qhia txog qhov sib txawv ntawm homologous recombination thiab site-specific recombination.
