Qhov sib txawv tseem ceeb ntawm serine thiab tyrosine recombinase yog tias hauv serine recombinase, serine yog nucleophilic amino acid siv los ntawm enzyme los tua DNA thaum lub sij hawm qhov chaw tshwj xeeb recombination, thaum nyob rau hauv tyrosine recombinase, tyrosine yog nucleophilic amino acid siv. los ntawm enzyme los tua DNA thaum lub sij hawm qhov chaw tshwj xeeb recombination.
Site-specific recombination (conservative site-specific recombination) yog ib hom kev sib txuas ntawm caj ces uas DNA sib pauv hloov pauv ntawm ntu uas muaj qee qhov kev sib luag ntawm homology. Site-specific recombinases tswj lub site-specific recombination txheej txheem. Cov enzymes no tau muab faib ua ob tsev neeg ua serine recombinase tsev neeg thiab tyrosine recombinase tsev neeg. Cov npe los ntawm kev txuag nucleophilic amino acid residue tam sim no nyob rau saum toj no ob chav kawm ntawm recombinases uas yog siv los tua DNA thiab ua covalently txuas rau nws thaum lub sij hawm strand pauv nyob rau hauv qhov chaw tshwj xeeb recombination.
YSerine Recombinase yog dab tsi?
Nyob rau hauv qhov chaw tshwj xeeb recombination txoj kev, ob lub DNA luv luv ('qhov chaw') raug txiav ntawm cov ntsiab lus tshwj xeeb hauv ob qho tib si, thiab qhov txiav xaus tau rov qab mus rau cov neeg koom tes tshiab. Qhov chaw tshwj xeeb recombination technologies yog cov cuab yeej genomic hauv genetic engineering uas nyob ntawm cov enzymes recombinase los hloov cov phiaj xwm ntawm DNA. Ntau qhov chaw tshwj xeeb recombination systems tau pom los ua cov DNA rearrangements rau ntau lub hom phiaj. Tab sis tag nrho cov recombinase enzymes no yog ob tsev neeg. Serine recombinase yog ib qho ntawm lawv. Lawv tuaj yeem kho kom haum rau peb hom DNA rov kho dua: kev sib koom ua ke, kev txiav tawm thiab kev hloov pauv.
Daim duab 01: Serine Recombinase
Qhov chaw tshwj xeeb recombinases zoo li serine recombinase ua DNA rov kho dua los ntawm kev lees paub thiab khi rau qhov luv luv DNA sib lawv liag (lub hom phiaj ntawm qhov chaw), uas lawv tshem tawm DNA qaum. Tom qab ntawd, kev sib pauv ntawm ob DNA helices tshwm sim thiab rov koom nrog DNA strands tshwm sim. Hauv serine recombinase, serine yog cov amino acid uas siv los ntawm cov enzyme los tua DNA thaum lub sij hawm qhov chaw tshwj xeeb recombination. Thaum lub sij hawm DNA cleavage, cov protein-DNA daim ntawv cog lus yog tsim los ntawm cov tshuaj tiv thaiv transesterification. Cov phosphodiester daim ntawv cog lus yog hloov los ntawm phosphoserine daim ntawv cog lus ntawm 5'phosphate pawg ntawm qhov chaw cleavage thiab pawg hydroxyl ntawm ib qho kev txuag serine residue. Daim ntawv cog lus tshiab khaws lub zog uas siv rau hauv kev tshem DNA ntawm lub hom phiaj. Lub zog no tom qab siv los koom nrog DNA mus rau pawg deoxyribose hydroxyl coj mus rau lwm qhov chaw. Piv txwv ntawm serine recombinases yog serine resolvases / invertases thiab serine integrases.
YTyrosine Recombinase yog dab tsi?
Tyrosine recombinase yog lwm chav kawm enzyme uas tswj xyuas qhov chaw tshwj xeeb recombination. Raws li tau piav qhia yav dhau los, tyrosine recombinase tseem ua rau DNA rov kho dua qub los ntawm kev lees paub thiab khi rau qhov luv luv DNA sib lawv liag (lub hom phiaj ntawm qhov chaw), ntawm qhov uas lawv cleave DNA qaum. Tom qab ntawd, sib pauv ntawm ob DNA helices thiab rov koom nrog DNA strands tshwm sim.
Daim duab 02: Tyrosine Recombinase
Tab sis hauv tyrosine recombinase, tyrosine yog cov amino acid uas siv los ntawm enzyme los tua DNA thaum lub sij hawm qhov chaw recombination. Lub hom phiaj ntawm cov chav kawm no yog kev txuag nucleophilic tyrosine residue tawm tsam cov scissile DNA-phosphate los ua ib qho kev sib txuas ntawm 3'-phosphotyrosine. Tyrosine recombinases (A1) thiab tyrosine integrases (A2) yog cov enzymes tseem ceeb hauv tyrosine recombinase enzyme tsev neeg.
Dab tsi yog qhov zoo sib xws ntawm Serine thiab Tyrosine Recombinase?
- Lawv yog ib chav kawm ntawm recombinase enzymes.
- Ob leeg tswj hwm qhov chaw tshwj xeeb recombination.
- YLawv yog cov protein.
- Cov tshuaj tiv thaiv kab mob tseem ceeb yog tib yam rau ob leeg.
- Ob qho tib si catalyze transesterification cov tshuaj tiv thaiv.
Qhov txawv ntawm Serine thiab Tyrosine Recombinase yog dab tsi?
Nyob rau hauv serine recombinase, serine yog nucleophilic amino acid uas yog siv los ntawm enzyme los tua DNA thaum lub sij hawm qhov chaw tshwj xeeb recombination. Ntawm qhov tod tes, hauv tyrosine recombinase, tyrosine yog nucleophilic amino acid uas yog siv los ntawm enzyme los tua DNA thaum lub sij hawm qhov chaw tshwj xeeb recombination. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm serine thiab tyrosine recombinase. Lub hom phiaj ntawm serine recombinase yog tias nws tsim ib daim ntawv cog lus phosphoserine nrog DNA, thaum tyrosine recombinase tsim ib daim ntawv cog lus phosphotyrosine nrog DNA thaum lub sij hawm qhov chaw tshwj xeeb recombination txheej txheem.
Cov hauv qab no qhia txog qhov sib txawv ntawm serine thiab tyrosine recombinase hauv daim ntawv teev npe.
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Qhov sib txawv ntawm Serine thiab Tyrosine Recombinase hauv Daim Ntawv Tabular
Summary – Serine vs Tyrosine Recombinase
Recombination tuaj yeem tshwm sim ntawm cov molecules zoo sib xws ntawm DNA xws li homologues recombination lossis dissimilar molecules xws li cov tsis sib xws. Site-specific recombination yog ib hom kev sib txuas ntawm cov noob caj noob ces nyob rau hauv uas DNA strand sib pauv yuav siv qhov chaw ntawm ntu uas muaj tsawg kawg yog qee qhov kev sib luag ntawm homology. Nws yog catalysed los ntawm recombinases. Feem ntau cov recombinases tau muab faib ua ob tsev neeg: serine recombinase thiab tyrosine recombinase. Serine recombinase siv serine ua cov nucleophilic amino acid los tua DNA thaum lub sij hawm qhov chaw tshwj xeeb recombination txheej txheem. Tyrosine recombinase siv tyrosine li nucleophilic amino acid los tua DNA thaum lub sij hawm qhov chaw tshwj xeeb recombination txheej txheem. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm serine thiab tyrosine recombinase.
