Qhov sib txawv tseem ceeb ntawm cov khoom sib xyaw ua ke thiab IS cov ntsiab lus yog cov khoom sib xyaw transposons yog ib hom kev hloov pauv uas muaj cov khoom siv txuas ntxiv xws li cov tshuaj tiv thaiv kab mob, thaum IS cov ntsiab lus (los yog cov ntsiab lus Insertion Sequence) yog cov khoom sib txuas uas muaj cov noob nkaus xwb. code transposase uas catalyze cov kev hloov pauv.
Ib transposon yog ib feem ntawm DNA uas tuaj yeem hloov nws txoj haujlwm hauv cov kab mob genome. Yog li ntawd, lawv yog mobile DNA sequences. Lawv txav mus rau qhov chaw tshiab ntawm cov genome, ua rau kev hloov pauv ntawm cov kab mob genome, ua rau muaj kev hloov pauv tseem ceeb hauv cov ntaub ntawv caj ces. Transposons tseem hu ua dhia caj ces vim lawv tuaj yeem thaiv qhov kev hloov pauv ntawm cov noob thiab rov kho cov khoom siv caj ces ntawm cov kab mob. Tsis tas li ntawd, lawv muaj lub luag haujlwm rau kev txav ntawm cov tshuaj tiv thaiv, tshuaj tua kab mob ntawm cov plasmids thiab chromosomes. Composite transposons thiab IS cov ntsiab lus yog ob hom mobile genetic ntsiab pom nyob rau hauv cov kab mob. Ob leeg tuaj yeem txav los ntawm ib qho chaw mus rau lwm qhov hauv genome.
Composite Transposons yog dab tsi?
Ib qho sib xyaw ua ke yog ib ntu ntawm DNA flanked los ntawm ob daim ntawv theej ntawm cov khoom sib txuas zoo sib xws. Muaj lub hauv paus protein-coding cheeb tsam nyob rau hauv composite transposon. Cov noob feem ntau yog cov tshuaj tiv thaiv kab mob. Lawv kuj tuaj yeem muaj cov noob catabolic. Ntxiv mus, composite transposon muaj ob inverted repeats. Tag nrho qhov ntev ntawm qhov sib xyaw transposon txav mus ua ib chav ua tiav.
Daim duab 01: Composite Transposon
Tn10 yog qhov sib xyaw ua ke. Nws muaj 6.5 kb hauv nruab nrab coding cheeb tsam (tetracycline-resistant gene) thiab 1.4 kb inverted insertion ib ntu ntawm txhua qhov kawg. IS cov khoom muab transposase. Transposase yog ib qho enzyme uas catalyzes lub zog ntawm transposon. Composite transposons tuaj yeem nthuav dav, tsim cov primers rau IS cov ntsiab lus.
Dab tsi yog Elements?
Insertion sequence elements or IS element is a type of mobile genetic element. Lawv yog ib qho yooj yim daim ntawv ntawm transposons. Lawv nqa tsuas yog cov noob coding rau transposase enzymes. Transposase enzymes catalyze cov kev hloov pauv ntawm transposons. Structureally, coding sequence ntawm IS ntsiab yog flanked los ntawm ob inverted repeats. Piv txwv li, IS lub ntsiab lus hu ua IS911 yog flanked los ntawm ob 36bp inverted rov extremities.
IS cov ntsiab lus yog cov khoom siv hluav taws xob ntau tshaj plaws hauv cov kab mob genome. Yog li ntawd, lawv tseem ceeb heev rau prokaryotic genome lub koom haum thiab evolution. Tsis tas li ntawd, IS cov ntsiab lus tshwm sim ua ib feem ntawm kev sib xyaw transposons. Feem ntau, ob lub ntsiab lus IS pom flanking cov coding ib ntus ntawm cov khoom sib txuas. Tab sis, unit transposons tsis nqa flanking IS ntsiab.
Vim tias yog cov ntsiab lus tuaj yeem txav mus rau hauv cov genome, lawv tuaj yeem cuam tshuam cov txheej txheem coding ntawm lwm cov noob thiab inactivate cov noob los ntawm thaiv kev qhia.
Dab tsi yog qhov zoo sib xws ntawm Composite Transposons thiab IS Elements?
- Ob qho tib si sib xyaw transposons thiab IS cov ntsiab lus tuaj yeem hloov txoj haujlwm hauv cov kab mob genome.
- Thaj chaw coding hauv ob qho tib si transposons feem ntau yog flanked los ntawm kev rov ua dua.
- Ib qho sib xyaw ua ke yog flanked los ntawm ob lub ntsiab lus cais.
Qhov txawv ntawm Composite Transposons thiab IS Elements yog dab tsi?
Composite transposons yog cov noob caj noob ces uas muaj ob qhov sib txuas (ISs) feem ntau flanking ib lossis ntau cov tshuaj tiv thaiv kab mob. Ntawm qhov tod tes, IS cov ntsiab lus yog ib hom kev hloov pauv yooj yim uas muaj cov noob coding rau transposase enzyme rau catalyze transposition. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm kev sib xyaw transposons thiab IS cov ntsiab lus.
Hauv qab no yog cov npe ntawm qhov sib txawv ntawm cov khoom sib xyaw ua ke thiab IS cov ntsiab lus hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – Composite Transposons vs IS Elements
Ib kab mob genome muaj cov kab mob caj ces uas yog lub xov tooj ntawm tes. Composite transposons thiab IS cov ntsiab lus yog ob hom mobile genetic ntsiab. Composite transposons nqa cov tshuaj tua kab mob-tiv taus cov noob thiab flanking IS cov ntsiab lus. IS cov ntsiab lus nqa cov noob caj noob ces los tsim cov transposase uas catalyzes lub zog ntawm transposons. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm kev sib xyaw transposons thiab IS cov ntsiab lus.
