Qhov sib txawv tseem ceeb ntawm cov roj chromatography thiab huab hwm coj spectrometry yog cov roj chromatography yog ib qho tseem ceeb hauv kev sib cais cov khoom hauv ib qho kev sib tov, whereas huab hwm coj spectrometry yog qhov tseem ceeb hauv kev xam qhov hnyav molecular ntawm cov qauv piv txwv.
Feem ntau, cov roj chromatography yog siv nrog rau qhov loj spectrometry vim tias peb tuaj yeem cais cov khoom sib xyaw ua ke siv cov roj chromatography thiab tuaj yeem txheeb xyuas cov khoom siv loj spectrometry.
Gas Chromatography yog dab tsi?
Gas chromatography yog cov txheej txheem kev tshuaj xyuas uas siv lub sijhawm txawb thiab lub sijhawm nyob ruaj khov yog siv qhov chaw txawb ntawm lub xeev roj. Cov txheej txheem chromatographic yog ib qho kev ntsuam xyuas uas siv los cais, txheeb xyuas, thiab qee zaum ntsuas cov khoom hauv qhov sib tov. Muaj ob hom xws li roj-khoom chromatography thiab roj-kua chromatography.
Nyob rau hauv cov roj-khoom chromatography, cov theem nyob ruaj khov yog nyob rau hauv cov khoom-lub xeev thiab cov theem mobile yog nyob rau hauv lub gaseous lub xeev. Ntawm no, roj-khoom chromatography yog siv rau kev sib cais ntawm volatile Cheebtsam nyob rau hauv ib tug sib tov. Cov txheej txheem no suav nrog ob qho tib si sib xyaw thiab theem mobile hauv lub xeev gaseous. Lub sijhawm txawb thiab qhov sib tov peb yuav sib cais ua ke nrog ib leeg, thiab tom qab ntawd qhov sib tov no dhau los ntawm cov khoom nyob ruaj khov. Cov theem nyob ruaj khov yog siv rau sab hauv phab ntsa ntawm lub raj hu ua chromatographic kem. Cov molecules ntawm cov theem nyob ruaj ruaj tuaj yeem cuam tshuam nrog cov molecules hauv theem mobile.
Daim duab 01: Txheej txheem ntawm Gas Chromatography
Nyob rau hauv cov roj-kua chromatography, cov theem nyob ruaj khov yog nyob rau hauv lub xeev cov kua thaum lub sij hawm mobile nyob rau hauv lub gaseous lub xeev. Nyob ntawd, cov theem nyob ruaj khov yog cov kua uas tsis muaj hluav taws xob. Peb yuav tsum siv cov theem no nyob ruaj ruaj rau ntawm phab ntsa sab hauv ntawm lub raj hu ua chromatographic kem. Tom qab ntawd, cov phab ntsa sab hauv ua raws li cov khoom txhawb nqa rau theem nyob ruaj khov. Hauv cov txheej txheem no, lub sijhawm txawb yog cov pa roj tsis zoo xws li Argon, Helium, lossis Nitrogen.
Mass Spectrometry yog dab tsi?
Mass spectrometry (feem ntau txhais los ntawm MS) yog cov txheej txheem hauv kev tshuaj ntsuam chemistry uas ntsuas qhov loj-rau-them piv ntawm ions. Qhov kawg tshwm sim ntawm cov txheej txheem no yog muab raws li ib tug loj spectrum uas tshwm raws li ib tug zajlus ntawm kev siv. Tsis tas li ntawd, peb yuav tsum kos cov phiaj xwm no raws li kev ua haujlwm ntawm qhov loj-rau-tus nqi piv. Hauv huab hwm coj spectrometry, qhov ntsuas peb siv rau kev ntsuas yog qhov loj spectrometer. Thaum peb qhia peb cov qauv rau hauv qhov ntsuas no, cov qauv molecules raug ionization. Thaum lub sij hawm no ionization, xaiv cov txheej txheem ionization kom zoo yog qhov tseem ceeb heev vim nws muaj kev cuam tshuam zoo rau qhov kawg. Yog tias peb siv cov roj reagent, piv txwv li. ammonia, nws yuav ua rau ionization ntawm cov qauv molecules los tsim cov ions zoo lossis tsuas yog cov ions tsis zoo, nyob ntawm qhov teeb tsa ntawm lub ntsuas.
Daim duab 02: Cov Txheej Txheem ntawm Mass Spectrometry
Qhov zoo ionization hauv huab hwm coj spectrometry cuam tshuam nrog kev tsim cov ions zoo rau kev txiav txim siab ntawm qhov loj-rau-tus nqi piv ntawm cov qauv molecules. Peb hu qhov zoo ion hom hauv huab hwm coj spectrometry. Peb tuaj yeem pom qhov zoo ion no li M-H+ Hauv cov txheej txheem no, peb tuaj yeem ntes cov ions hauv cov txiaj ntsig siab.
Tsis zoo ionization hauv huab hwm coj spectrometry cuam tshuam nrog kev tsim cov ions tsis zoo rau kev txiav txim siab ntawm qhov loj-rau-tus nqi piv ntawm cov qauv molecules. Peb hu qhov no tsis zoo ion hom hauv huab hwm coj spectrometry. Tsis tas li ntawd, peb tuaj yeem txheeb xyuas qhov tsis zoo ion no li M-H– Hauv cov txheej txheem no, peb tuaj yeem txheeb xyuas cov ions hauv cov txiaj ntsig siab.
Qhov txawv ntawm Gas Chromatography thiab Mass Spectrometry yog dab tsi?
Gas chromatography yog cov txheej txheem kev tshuaj xyuas uas siv cov theem ntawm lub xov tooj ntawm tes thiab cov theem nyob ruaj khov yog siv qhov twg theem mobile nyob hauv lub xeev roj. Mass spectrometry (feem ntau qhia los ntawm MS) yog ib qho txheej txheem hauv analytical chemistry uas ntsuas qhov loj-rau-them piv ntawm ions. Yog li ntawd, qhov sib txawv tseem ceeb ntawm cov roj chromatography thiab huab hwm coj spectrometry yog cov roj chromatography yog qhov tseem ceeb hauv kev sib cais cov khoom hauv ib qho sib tov, whereas huab hwm coj spectrometry yog qhov tseem ceeb hauv kev xam qhov hnyav molecular ntawm cov qauv piv txwv.
Cov hauv qab no infographic qhia txog qhov sib txawv ntawm cov roj chromatography thiab huab hwm coj spectrometry hauv daim ntawv teev npe.
Summary – Gas Chromatography vs Mass Spectrometry
Ntau zaus peb siv cov roj chromatography ua raws li qhov loj spectrometry txhawm rau cais cov khoom sib xyaw hauv qhov xav tau sib xyaw ua raws li lawv cov cim. Qhov sib txawv tseem ceeb ntawm cov roj chromatography thiab huab hwm coj spectrometry yog cov roj chromatography yog ib qho tseem ceeb hauv kev sib cais cov khoom hauv ib qho sib tov, whereas huab hwm coj spectrometry yog qhov tseem ceeb hauv kev xam qhov hnyav molecular ntawm cov qauv piv txwv.
