Qhov sib txawv ntawm qhov zoo thiab qhov tsis zoo oxidase kuaj yog qhov kev kuaj oxidase zoo qhia tias muaj cytochrome C oxidase hauv cov kab mob, qhov tsis zoo oxidase kuaj qhia tias tsis muaj cytochrome C oxidase.
Lub sij hawm kuaj oxidase feem ntau yog qhov tseem ceeb hauv microbiology thiab muaj cov ntawv thov hauv analytical chemistry thiab. Kev kuaj oxidase kuaj pom muaj cytochrome oxidase.
Kev kuaj Oxidase yog dab tsi?
Oxidase kuaj yog qhov tseem ceeb hauv kev txiav txim siab seb tus kab mob puas tuaj yeem tsim cytochrome C oxidases lossis tsis. Cov txheej txheem kev tshuaj ntsuam no siv cov disks uas impregnated nrog reagents xws li TMPD lossis DMPD. Thaum oxidized, lub reagent yuav xiav rau maroon xim. Thaum nws nyob rau hauv lub xeev txo, cov reagent tsis muaj xim.
Daim duab 01: Xim Hloov hauv Oxidase Test
Cov kab mob uas muaj cytochrome C oxidases tuaj yeem ua rau kev thauj cov hluav taws xob los ntawm cov khoom siv pub dawb xws li NADH rau cov khoom siv hluav taws xob xws li oxygen. TMPD lossis qhov ntsuas reagent hauv oxidase test ua raws li cov khoom siv hluav taws xob tsim hluav taws xob; Yog li, oxidized reagent muab xim (los ntawm kev tsim cov xim sib xyaw indophenol xiav). Feem ntau, cov kab mob oxidase-zoo muaj xws li cov kab mob aerobic (cov kab mob no muaj peev xwm siv cov pa oxygen raws li lub davhlau ya nyob twg electron txais).
Kev kuaj pom zoo Oxidase yog dab tsi?
Kev kuaj oxidase zoo yog cov txheej txheem tshuaj ntsuam xyuas uas peb tuaj yeem txiav txim siab pom cov kab mob uas muaj cytochrome c oxidase enzyme. Qhov no txhais tau tias yog OX +. Cov kab mob no tuaj yeem siv cov pa oxygen rau kev tsim hluav taws xob los ntawm kev hloov cov pa oxygen rau hauv hydrogen peroxide lossis dej ntawm cov saw hlau hloov hluav taws xob. Feem ntau, cov kab mob ntawm Pseudomonasdaceae hom yog OX +. Tsis tas li ntawd, ntau cov kab mob Gram-negative, kauv nkhaus pas nrig-zoo li tus kab mob xws li Vibrio cholerae yog oxidase zoo.
Nugative Oxidase Test yog dab tsi?
Negative oxidase test yog ib qho kev tshuaj ntsuam xyuas uas peb tuaj yeem txiav txim siab qhov tsis muaj cytochrome c oxidase enzyme hauv cov qauv kab mob. Peb tuaj yeem txhais lo lus no ua OX-. Cov kab mob no tsis tuaj yeem siv cov pa oxygen rau lub zog tsim los ntawm cov saw hlau hloov hluav taws xob. Yog tias tsis yog, cov kab mob no siv cytochrome sib txawv rau kev hloov ntawm electrons rau oxygen. Feem ntau, Enterobacteriaceae yog oxidase tsis zoo.
Qhov txawv ntawm qhov zoo thiab qhov tsis zoo Oxidase Test yog dab tsi?
Kev kuaj Oxidase muaj txiaj ntsig los txiav txim siab seb tus kab mob puas tuaj yeem tsim cytochrome C oxidases lossis tsis. Qhov sib txawv tseem ceeb ntawm qhov zoo thiab qhov tsis zoo oxidase kuaj yog qhov kev kuaj oxidase zoo qhia tias muaj cytochrome C oxidase hauv cov kab mob, qhov tsis zoo oxidase kuaj qhia tias tsis muaj cytochrome C oxidase.
Ntxiv mus, hauv kev kuaj oxidase zoo, cov xim hloov pauv yog xiav rau maroon, thaum nyob rau hauv qhov tsis zoo oxidase kuaj, xim hloov tsis tshwm sim. Ntau cov kab mob Gram-negative thiab kauv-curved, rod-shaped kab mob xws li Vibrio cholerae yog oxidase-zoo thaum cov kab mob los ntawm Enterobacteriaceae hom yog oxidase-negative.
Cov infographic hauv qab no qhia txog qhov sib txawv ntawm qhov zoo thiab qhov tsis zoo oxidase kuaj hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – Positive vs Negative Oxidase Test
Kev kuaj Oxidase muaj txiaj ntsig los txiav txim siab seb tus kab mob puas tuaj yeem tsim cytochrome C oxidases lossis tsis. Qhov sib txawv tseem ceeb ntawm qhov zoo thiab qhov tsis zoo oxidase kuaj yog qhov kev kuaj oxidase zoo qhia tias muaj cytochrome C oxidase hauv cov kab mob, qhov tsis zoo oxidase kuaj qhia tias tsis muaj cytochrome C oxidase. Hauv kev kuaj oxidase zoo, kev hloov xim yog los ntawm xiav rau maroon, thaum nyob rau hauv qhov kev kuaj oxidase tsis zoo, kev hloov xim tsis tshwm sim.
