Qhov sib txawv tseem ceeb ntawm dATP thiab ddATP yog tias dATP tsis txiav DNA synthesis thaum ddATP tuaj yeem txiav tawm DNA synthesis. Qhov no yog vim ddATP muaj H atom txuas rau 3ʹ txoj haujlwm ntawm cov piam thaj pentose thaum dATP muaj OH pawg txuas rau 3ʹ txoj haujlwm.
dNTPs yog tsim cov blocks ntawm DNA. Muaj plaub hom dNTPs raws li purine lossis pyrimidine bases-dATP ib qho ntawm lawv. Lub hauv paus nitrogenous ntawm dATP yog adenosine. ddNTPs yog nucleotides siv hauv Sanger sequencing. Muaj plaub hom ddNTPs. ddATP yog ddNTPs.
dATP yog dab tsi?
Deoxyadenosine triphosphate yog nucleotide, uas yog lub tsev thaiv DNA. DNA polymerase enzyme siv dATP ua substrate rau DNA synthesis. dATP muaj peb yam: deoxyribose qab zib molecule, adenosine, thiab peb pawg phosphate. Yog li, nws yog purine nucleoside triphosphate. Cov tshuaj formula ntawm dATP yog C10H16N5O12 P3, thiab nws molecular mass yog 491.182.
Daim duab 01: dATP
dATP txawv ntawm ATP (adenosine triphosphate) hais txog cov khoom qab zib. ATP muaj ribose qab zib. dATP puag khub nrog uracil nucleotide thaum lub sijhawm hloov pauv. Ntxiv nrog rau kev koom tes hauv DNA synthesis, dATP tuaj yeem ua raws li lub zog-hloov molecule los tswj cov cell viability thiab. Ib qib siab ntawm daATP hauv lub cev yog tshuaj lom vim nws tuaj yeem ua raws li qhov tsis sib haum xeeb inhibitor rau enzyme ribonucleotide reductase. Nws tuaj yeem ua rau lub cev tsis muaj zog.
ddATP yog dab tsi?
Dideoxyadenosine triphoaphate lossis ddATP yog ib qho ntawm plaub hom nucleotides siv hauv Sanger sequencing txoj kev. Structureally, ddATP kuj muaj peb yam zoo ib yam li dATP. Lawv yog adenine puag, deoxyribose qab zib thiab peb phosphates.
Daim duab 02: ddATP
Tsis zoo li dATP, ddATP tsis muaj OH pawg ntawm 3ʹ txoj haujlwm ntawm cov piam thaj pentose txhawm rau tsim phosphodiester daim ntawv cog lus nrog cov nucleotide uas nyob ib sab thiab txuas ntxiv cov saw elongation. Nws muaj H atom hauv 3ʹ txoj hauj lwm. Yog li ntawd, ib zaug ddATP tau koom ua ke, DNA synthesis los txog qhov kawg. Nws yog lub hauv paus ntsiab lus ntawm Sanger sequencing. Yog li, ddATP ua haujlwm raws li cov saw elongation inhibitor hauv Sanger sequencing. Muaj plaub lub raj siv hauv Sanger sequencing thiab ddATP ntxiv rau ib lub raj.
DATP thiab ddATP yog dab tsi?
- dATP thiab ddATP yog nucleotides.
- Ob leeg yog tsim ntawm adenine, deoxyribose thiab peb phosphates.
- Lawv yog cov organic tebchaw.
- Ob leeg dATP thiab ddATP tau ntxiv rau hauv cov raj hauv Sanger sequencing.
Qhov txawv ntawm dATP thiab ddATP yog dab tsi?
dATP muaj OH pawg hauv 3ʹ txoj haujlwm ntawm cov piam thaj pentose thaum ddATP tsis muaj OH pawg hauv 3ʹ txoj haujlwm ntawm cov suab thaj pentose. Tsis tas li ntawd, dATP yog ib qho monomer siv hauv DNA synthesis thaum ddATP yog siv los txiav cov saw elongation thaum lub sij hawm Sanger sequencing. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm dATP thiab ddATP. Ntxiv mus, dATP tuaj yeem tsim phosphodiester bonds thaum ddATP tsis tuaj yeem tsim phosphodiester bonds.
Hauv qab infographic qhia ntau qhov sib txawv ntawm dATP thiab ddATP hauv daim ntawv teev npe rau ib sab ntawm kev sib piv.
Summary – dATP vs ddATP
Hauv luv luv, dATP yog qhov ua ntej rau DNA synthesis thaum ddATP yog nucleotide siv los txiav DNA synthesis hauv Sanger sequencing. Ntxiv mus, dATP muaj ib pab pawg OH nyob rau hauv 3ʹ txoj hauj lwm ntawm pentose qab zib thiab nws tso cai rau tsim ib tug phosphodiester daim ntawv cog lus nrog rau cov nucleotide tom ntej. Lub caij no, ddATP tsis muaj OH pawg hauv 3ʹ txoj haujlwm; li no, nws tsis tuaj yeem tsim ib daim ntawv cog lus phosphodiester nrog cov nucleotide tom ntej. Raws li qhov tshwm sim, DNA sib lawv liag nres nws elongation. Yog li, qhov no qhia txog qhov sib txawv ntawm dATP thiab ddATP.
