Qhov sib txawv tseem ceeb ntawm hypersil thiab inertsil kem yog tias hypersil kem yog lub npe lag luam rau BDS kem whereas inertsil kem yog lub npe lag luam rau ODS kem. ODS kem yog hom rov qab-theem HPLC kem uas muaj cov pab pawg dawb -OH ua haujlwm thaum BDS kem yog lwm hom rov qab-theem HPLC kem uas tau thaiv -OH pawg.
Rov qab-theem HPLC yog cov txheej txheem chromatographic uas siv cov theem hydrophobic nyob ruaj khov. Qhov chaw nyob ruaj khov no ua haujlwm zoo rau kev khaws cia ntawm feem ntau cov organic analytes. Lub xov tooj ntawm tes ntawm theem rov qab HPLC yog polar. Muaj ntau hom kab siv rau cov txheej txheem chromatographic no. Hypersil (los yog BDS) thiab inertsil (lossis ODS) yog ob kab ntawv.
Hypersil Kab yog dab tsi?
Hypersil kem lossis BDS kem yog hom rov qab-theem HPLC kem uas tau thaiv -OH pawg. Hauv lwm lo lus, pawg hydroxyl hauv kab ntawv no yog deactivated / tsis pub dawb. Peb kuj hu nws BDS C18 kem vim hais tias kem no tau ntim nrog octadecasilane chains. Lo lus BDS sawv cev rau Base Deactivated Silica; yog li ntawd, peb tseem tuaj yeem muab cov npe no ua cov kab ntawv kawg.
kab ntawv no tseem ceeb heev hauv chromatography vim nws muaj nws cov seem silanol pawg deactivated los ntawm capping. Yog li ntawd, muaj tsawg kawg nkaus residual silanol kev ua. Tsis tas li ntawd, kab lus no tshwj xeeb rau kev txheeb xyuas cov ntsiab lus sib xyaw. Ntawm no, cov hauv paus muaj kev cuam tshuam nrog Si-OH pawg hauv silica packing. BDS txhua kab yog tsim los txo qhov ncov tailing, uas yog ib qho teeb meem loj hauv chromatography (tsis tuaj yeem paub txog qee qhov ncov).
Dab tsi yog Inertsil Kab?
Inertsil kem lossis ODS kem yog hom rov qab-theem HPLC kem uas muaj cov pab pawg ua haujlwm dawb -OH. Peb tuaj yeem sau nws li C18 kem vim nws muaj octadecasilane chains. Hauv lwm lo lus, peb tuaj yeem sau ib kab C18 nrog ntim ntawm octadecysilyl pawg (cov no kuj muaj npe hu ua ODS pawg lossis C18 pawg) uas muaj kev sib raug zoo rau cov neeg nqa khoom silica gel. Cov kab ntawv hypersil ODS no tshwj xeeb tseem ceeb hauv cov txheej txheem rov qab-theem chromatographic. Tsis tas li ntawd, hom kab no muaj cov phaj siab theoretical thiab tseem qhia tau qhov sib npaug nrawm. Txij li cov kab no tsuas yog xav tau tus nqi qis rau kev khiav lag luam, lawv feem ntau siv nyob rau hauv reverse-theem chromatography.
Daim duab 1: Ib qho HPLC Instrument
Txawm li cas los xij, muaj ntau qhov tsis zoo ntawm kev siv cov kab ODS no hauv chromatography. Piv txwv li, nws tag nrho elution yog ceev heev, yog li nws yog ib qho nyuaj rau cais ib co Cheebtsam nyob rau hauv ib tug sib tov, thiab stabilization ntawm kem yuav siv sij hawm ib tug sib piv ntev.
Thaum xav txog cov qauv tshuaj ntawm ODS kem, nws muaj cov pab pawg hydroxyl (-OH) txuas rau saum npoo ntawm silica gel carrier qhov twg nws muaj cov qauv Si-OH. Cov qauv no hu ua "silanol". Hauv ODS kem packing, kev ntim khoom yog ua los ntawm kev sib txuas cov pab pawg ODS rau cov silanol los ntawm cov tshuaj tiv thaiv. Cov pab pawg ODS no loj heev thiab tsis muaj zog heev. Yog li ntawd, muaj ntau yam tsis muaj kev cuam tshuam silanol pawg nyob rau hauv kab ntawv no. Txawm li cas los xij, cov silanol dawb no tuaj yeem ua rau muaj kev ua yuam kev thaum lub sijhawm tshuaj ntsuam, yog li peb yuav tsum tau ntim cov pab pawg no nrog qee qhov sib txuas xws li TMS (trimethylsilyl) pawg, uas tsis yog loj tab sis muaj zog heev. Cov txheej txheem no hu ua qhov kawg-capping.
Qhov txawv ntawm Hypersil thiab Inertsil Kab yog dab tsi?
Hypersil thiab inertsil kab yog cov npe lag luam, thiab cov no yog cov kab rov qab-theem HPLC tseem ceeb. Qhov sib txawv tseem ceeb ntawm hypersil thiab inertsil kem yog tias hypersil kem yog lub npe lag luam rau BDS kem whereas inertsil kem yog lub npe lag luam rau ODS kem. Hypersil kem lossis BDS kem yog hom rov qab-theem HPLC kem uas tau thaiv -OH pawg. Inertsil kem lossis ODS kem yog hom rov qab-theem HPLC kem uas muaj cov pab pawg ua haujlwm pub dawb -OH.
Cov hauv qab no infographic qhia txog qhov sib txawv ntawm hypersil thiab inertsil kem nyob rau hauv daim ntawv tabular.
Summary – Hypersil vs Inertsil Kab
Hypersil thiab inertsil kab yog cov npe lag luam, thiab cov no yog cov kab rov qab-theem HPLC tseem ceeb. Qhov sib txawv tseem ceeb ntawm hypersil thiab inertsil kem yog tias hypersil kem yog lub npe lag luam rau BDS kem whereas inertsil kem yog lub npe lag luam rau ODS kem.
