Qhov sib txawv tseem ceeb ntawm cov protein denaturation thiab hydrolysis yog tias nyob rau hauv cov protein denaturation, cov protein poob nws cov qauv peb sab thiab ua haujlwm thaum nyob hauv cov protein hydrolysis, cov proteins feem ntau hloov mus rau hauv lawv cov amino acids los ntawm enzymes.
Proteins yog macromolecules uas muaj cov amino acids. Hauv cov protein, muaj ntau pua lossis txhiab tus amino acids khi rau ib leeg. Txhawm rau tsim cov protein ua haujlwm, polypeptide chains cuam tshuam nrog ib leeg thiab tsim kev sib koom ua ke los tsim cov protein cov qauv peb-seem tshwj xeeb. Cov duab kawg ntawm cov protein yog qhov muaj zog tshaj plaws thiab ruaj khov. Ntxiv mus, nws yog biologically active. Muaj ntau txhiab daim ntawv cog lus hauv cov protein 3D qauv. Qee yam cuam tshuam rau cov qauv peb-dimensional lossis cov duab kawg ntawm cov protein. Yog li ntawd, cov protein poob nws cov duab thiab ua haujlwm. Peb hu nws denaturation protein. Protein hydrolysis lov cov protein rau hauv nws tus kheej cov amino acids. Nws yog ua los ntawm enzymes.
YProtein Denaturation yog dab tsi?
Proteins muaj lawv cov duab peb sab tshwj xeeb. Cov qauv peb-dimensional tseem ceeb heev rau cov protein rau nws cov haujlwm tshwj xeeb. Txawm li cas los xij, vim muaj qhov sib txawv, cov protein tuaj yeem poob nws cov duab thiab ua haujlwm. Peb hu cov txheej txheem no protein denaturation. Proteins poob lawv cov duab thaum kev sib txuas thiab kev sib cuam tshuam lub luag haujlwm rau cov txheej txheem theem nrab thiab qib theem ntawm cov protein raug cuam tshuam. Kev kub siab, pH hloov pauv, qee yam tshuaj lom neeg thiab raug hluav taws xob, thiab lwm yam, tuaj yeem tsim cov proteins. Ntxiv mus, cov proteins tuaj yeem raug kho los ntawm kev kho nrog alkaline lossis acid, oxidizing lossis txo cov neeg ua haujlwm, thiab qee cov organic solvents xws li ethanol lossis acetone. Urea thiab guanidinium chloride yog ob feem ntau siv denaturing agents.
Daim duab 01: Protein Denaturation
Protein denaturation yog irreversible nyob rau hauv huab mob. Tsis tshua muaj, tus qauv qub ntawm cov protein denatured tuaj yeem rov tsim dua. Yog tias cov qauv tseem ceeb thiab lwm yam tsis zoo, qee zaum muaj peev xwm ntawm renaturation (rov qab denaturation).
YProtein Hydrolysis yog dab tsi?
Y Protein hydrolysis yog hloov cov proteins rau hauv cov amino acids thiab peptides. Nws tuaj yeem ua tau enzymatically thiab chemically. Thaum lub sij hawm hydrolysis, bonds ntawm cov amino acids raug cuam tshuam los tsim cov amino acids dawb. Protein hydrolysis tshwm sim hauv cov kab mob vim yog cov enzymes xws li pancreatic proteases, thiab lwm yam. Thaum peb noj cov zaub mov muaj protein ntau, lawv tau hydrolyzed rau hauv me me peptides thaum lub sij hawm zom zaub mov los ntawm enzymes.
Daim duab 02: Protein Hydrolysis
Protein hydrolysis yog qhov tseem ceeb vim nws tso cai rau kev sib cais ntawm tus kheej cov amino acids. Piv txwv li, histidine tuaj yeem cais tawm ntawm cov qe ntshav liab. Ib yam li ntawd, cystine tuaj yeem raug cais tawm los ntawm hydrolysis ntawm cov plaub hau. Qee qhov xwm txheej, thaum nws xav tau los ntsuas tag nrho cov amino acid cov ntsiab lus hauv cov qauv, ntau cov txheej txheem hydrolysis yuav tsum tau ua. Acid hydrolysis yog cov txheej txheem feem ntau ntawm kev txheeb xyuas cov protein. Ntxiv mus, alkaline hydrolysis kuj tuaj yeem ua haujlwm los ntsuas tryptophan. Yog li, txoj kev xaiv rau hydrolysis ntawm cov protein nyob ntawm lawv qhov chaw.
Dab tsi zoo sib xws ntawm Protein Denaturation thiab Hydrolysis?
- Protein denaturation thiab hydrolysis ua rau hloov mus rau hauv cov qauv protein.
- Denaturation feem ntau ua ntej hydrolysis.
- Kub thiab pH yog ob qho laj thawj cuam tshuam rau ob qho tib si denaturation thiab hydrolysis.
Qhov txawv ntawm Protein Denaturation thiab Hydrolysis yog dab tsi?
Protein denaturation ua rau cov proteins poob lawv cov duab peb sab thaum cov protein hydrolysis tsim cov amino acids dawb thiab peptides. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm cov protein denaturation thiab hydrolysis. Ntxiv mus, protein denaturation tshwm sim vim kub kub, pH hloov, tej yam tshuaj, thiab lwm yam. Protein hydrolysis yuav ua tau siv enzymes thiab tshuaj.
Hauv qab infographic tabulates ntau qhov sib txawv ntawm cov protein denaturation thiab hydrolysis.
Summary – Protein Denaturation vs Hydrolysis
Protein denaturation yog hais txog kev cuam tshuam ntawm theem nrab lossis qib theem ntawm cov protein, tshwj xeeb tshaj yog kev puas tsuaj ntawm alpha-helix thiab beta nplooj ntawv. Txawm li cas los xij, cov qauv tseem ceeb ntawm cov protein tseem nyob txawm tias tom qab denaturation. Qhov kev soj ntsuam tshaj plaws thaum denaturing cov protein yog cov nag lossis daus. Protein hydrolysis hais txog kev hloov pauv ntawm cov protein rau hauv lawv cov amino acids thiab peptides. Nws yog ib qho txheej txheem tseem ceeb thaum cais cov amino acids ib leeg. Yog li, qhov no qhia txog qhov sib txawv ntawm cov protein denaturation thiab hydrolysis.
