Qhov sib txawv tseem ceeb ntawm denaturation thiab renaturation ntawm cov protein yog tias denaturation yog qhov poob ntawm haiv neeg 3D qauv ntawm cov protein thaum renaturation yog hloov dua siab tshiab ntawm cov protein denatured rau hauv nws haiv neeg 3D qauv.
Proteins yog ib qho ntawm cov macromolecules tseem ceeb tam sim no hauv cov kab mob muaj sia. Cov molecules tseem ceeb xws li enzymes, cov khoom siv thiab cov tshuaj tiv thaiv kab mob, thiab lwm yam yog cov proteins. Qhov tseeb, cov proteins yog ib qho tseem ceeb macronutrient. Amino acids yog lub tsev blocks ntawm cov proteins. Ib qho amino acid ib ntus lossis cov saw hlau polypeptide tsim kev sib cuam tshuam thiab folds rau hauv nws cov qauv quaternary lossis tertiary qauv lossis cov qauv theem nrab uas muaj kev lom zem.
Thaum cov protein tau txais nws cov qauv 3D, nws ua haujlwm zoo. Qee yam tuaj yeem nthuav tawm lossis tshem tawm cov protein. Yog li ntawd, denaturation yog cov txheej txheem uas cov protein poob nws haiv neeg 3D qauv. Vim denaturation, cov proteins ua rau lom tsis muaj zog. Nyob rau hauv sib piv, renaturation yog tus txheej txheem uas ib tug denatured protein yuav hloov mus rau hauv nws tus thawj 3D qauv.
Denaturation ntawm Protein yog dab tsi?
Denaturation yog txheej txheem los ntawm cov protein uas poob nws cov qauv quaternary, tertiary qauv lossis cov qauv theem nrab uas ua rau nws muaj kev lom zem. Thaum lub sij hawm denaturation, lub zog uas tuav lub 3D qauv ntawm cov protein molecule raug cuam tshuam. Yog li ntawd, cov protein molecule poob nws cov khoom ntuj thiab nws cov khoom lom. Proteins ua rau lub cev ua haujlwm vim muaj protein ntau. Denaturation ua rau nthuav tawm ntawm cov saw hlau polypeptide, ua rau muaj kev cuam tshuam ntawm 3D qauv ntawm cov protein. Thaum lawv poob lawv cov qauv 3D, lawv ua haujlwm tsis ua haujlwm lossis tsis ua haujlwm.
Daim duab 01: Denaturation of Protein
Denaturation ntawm cov proteins tuaj yeem ua tiav los ntawm kev siv qee qhov kev ntxhov siab sab nraud lossis cov khoom sib xyaw xws li cov kua qaub lossis cov hauv paus, cov ntsev tsis muaj zog, cov kuab tshuaj organic, hluav taws xob lossis cua sov, thiab lwm yam. Cells tuag thaum cov proteins ntawm tes. denatured. Qhov tseem ceeb tshaj plaws, thaum lub protein yog denatured, nws tsis tuaj yeem ua tiav nws txoj haujlwm. Piv txwv li, thaum cov enzymes yog denatured, lawv tsis tuaj yeem ua rau cov tshuaj tiv thaiv biochemical. Lawv kuj pom tias poob ntawm solubility rau protein aggregation.
Renaturation ntawm Protein yog dab tsi?
Renaturation ntawm ib qho protein yog kev hloov pauv ntawm cov protein denatured rov qab rau hauv nws cov qauv 3D. Yog li, nws cuam tshuam nrog kev tsim kho ntawm cov protein molecule tom qab poob nws cov qauv qub. Renaturation yog tus txheej txheem rov qab ntawm denaturation. Renaturation yog qee zaum thim rov qab. Txawm li cas los xij, renaturation tsis yog ib qho yooj yim thiab yooj yim li denaturation. Ib txoj hauv kev ntawm renaturing ib cov protein yog tshem tawm SDS thiab denaturing agents tom qab denaturation thaum lub sij hawm PAGE los yog IEF protein identification. Thaum cov xwm txheej ntawm lub cev raug muab tso rov qab, cov protein folding tuaj yeem tshwm sim thiab rov ua nws qhov qub 3D conformation.
Dab tsi yog qhov zoo sib xws ntawm Denaturation thiab Renaturation ntawm Protein?
- Renaturation is the inverse process of denaturation.
- Denaturation rhuav tshem cov qauv 3D thaum renaturation rov ua dua 3D qauv.
Qhov txawv ntawm Denaturation thiab Renaturation ntawm Protein yog dab tsi?
Denaturation yog cov txheej txheem ntawm cov protein poob nws cov qauv quaternary, tertiary qauv lossis cov qauv theem nrab, uas ua rau nws muaj kev lom zem. Ntawm qhov tod tes, renaturation yog hloov dua siab tshiab ntawm cov protein denatured rau hauv nws cov qauv 3D. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm denaturation thiab renaturation ntawm protein.
Ntxiv mus, denaturation ua rau poob ntawm kev ua haujlwm lom neeg ntawm cov protein, thaum renaturation tuaj yeem rov ua kom muaj peev xwm ua haujlwm ntawm cov protein.
Hauv qab infographic qhia ntau qhov sib txawv ntawm denaturation thiab renaturation ntawm protein.
Summary – Denaturation vs Renaturation of Protein
Denaturation thiab renaturation yog ob txoj kev cuam tshuam feem ntau rau cov proteins thiab nucleic acids. Vim yog denaturation, cov proteins poob lawv cov kev ua haujlwm thiab kev lom zem 3D qauv. Nyob rau hauv sib piv, vim renaturation, ib tug denatured protein tau txais nws haiv neeg 3D qauv rov qab. Yog li, qhov no yog qhov sib txawv tseem ceeb ntawm denaturation thiab renaturation ntawm cov protein.
